13 results about "Cell membrane structures" patented technology

The application discloses medical use of gelated cells loaded with sulfonated chitosan in treatment of atherosclerosis, and belongs to the technical field of biological medicine. The gelated cells loaded with sulfonated chitosan take macrophages as host cells, and intracellular cross-linking forms a photocrosslinking hydrogel network composed of methacrylated hyaluronic acid and sulfonated chitosan. The gelated cells completely retain cell membrane structure and membrane surface protein expression, lose the proliferation ability, and have excellent blood lipid selective adsorption capacity and foam cell lipid metabolism regulation capacity. In-vivo and in-vitro experiments prove that the gelated cells can significantly reduce blood lipid levels of atherosclerosis model animals, reduce aortic plaque area, and have good biological safety, thereby providing a new strategy and candidate drug for prevention and treatment of atherosclerosis, and having a good clinical application prospect.

The utility model provides a kind of mixing device for freeze-drying probiotic production line, it is related to probiotic freeze-drying production device technical field, including mixing barrel, stirring device and distributor, the mixing barrel top portion is provided with top cover, mixing barrel bottom is equipped with discharge gate, stirring device is evenly spaced transversely installed in mixing barrel around mixing barrel axis line, the top cover of distributor is coaxial with the mixing barrel and is installed, the distributor bottom is provided with several groups of material distribution hole corresponding with the direction of stirring device in mixing barrel, the application can solve the problem that traditional liquid needs to be combined with the mode that single-point feeding and high-speed paddle type mechanical stirring are combined to form local concentration too high, mixing is not uniform and high shear force destroys bacterial cell membrane structure, causes the problem of active bacteria survival rate decline.

This invention discloses an umbilical cord mesenchymal stem cell membrane for treating hair loss, its preparation method, and its application, belonging to the field of biomedical technology. The preparation method of the umbilical cord mesenchymal stem cell membrane includes the following steps: obtaining human umbilical cord mesenchymal stem cells, digesting, centrifuging, washing, and resuspending them to obtain a resuspended cell solution; subjecting the resuspended cell solution to repeated freeze-thaw cycles to break the cells, obtaining a cell lysate; and subjecting the cell lysate to differential centrifugation to obtain the umbilical cord mesenchymal stem cell membrane. This umbilical cord mesenchymal stem cell membrane retains the natural cell membrane structure and functional proteins, has a negatively charged surface, and exhibits good dispersibility and stability. It can improve the survival rate of dermal papilla cells without introducing live cells and effectively regulate the skin microecology and metabolite balance, demonstrating high safety, good biocompatibility, and excellent hair growth promotion effects.

This invention relates to the field of agricultural biotechnology, and in particular to a stress-resistant, antifreeze, and preservative biofilm formulation and its preparation method. The stress-resistant, antifreeze, and preservative biofilm formulation is composed of the following raw materials in parts by weight: 13-28 parts film-forming matrix, 5.5-11.5 parts antifreeze active component, 2.51-6.05 parts stress-resistant active component, 7-14 parts crosslinking and plasticizer, 0.1-0.5 parts natural antibacterial agent, and 55-75 parts deionized water. This invention innovatively achieves a four-fold synergy of physical barrier, active stress resistance, efficient antifreeze, and natural antibacterial properties. The film-forming matrix forms a dense and breathable physical barrier, inhibiting moisture loss; the stress-resistant components construct an active stress-resistant system, enhancing the stress resistance of agricultural products; the antifreeze components achieve efficient antifreeze by inhibiting ice crystal formation and stabilizing cell membrane structure; and the natural antibacterial agent specifically inhibits postharvest pathogens such as Penicillium and Botrytis. The four components work synergistically to comprehensively solve multiple problems during the storage of agricultural products.

The application belongs to the technical field of microorganisms, and discloses application of shikimic acid in preparation of antibacterial preparations for inhibiting staphyloxin synthesis. It is found that shikimic acid can inhibit synthesis of staphyloxin in methicillin-resistant Staphylococcus aureus (MRSA), reduce tolerance of the bacteria to oxidative stress, induce accumulation of intracellular active oxygen, and damage cell membrane structure and function, including reducing cell membrane order, inducing membrane depolarization, increasing membrane permeability, and leading to ATP leakage and energy metabolism disorder. A new strategy and preparation direction are provided for clinical treatment of multi-drug resistant Staphylococcus aureus infection, and the application has important theoretical value and application prospect.

The invention discloses construction and application of an engineering strain capable of normally growing at 35 DEG C and realizing high yield of xanthan gum. The method comprises the following steps: by taking xanthomonas campestris XC1 as an initial strain, carrying out ARTP mutagenesis treatment, and screening a mutant strain which can normally grow under the condition of 35 DEG C; the mutant strain is subjected to multiple rounds of superposition transformation, and multiple physiological restrictions under high temperature stress are systematically broken by sequentially strengthening a protein folding system (grpE-ATGA-dnaK), activating outer membrane stress regulation (rpoE), constructing an anti-oxidation barrier (katB) and strengthening a cell membrane structure (polCDE); the finally obtained engineering strain shows the performance far better than that of an initial strain under the condition of 35 DEG C; the biomass of the strain is increased by 207.7% compared with that of an initial strain XC1, and the yield of xanthan gum is increased by 75.5% and reaches 25.8 g / L, which is consistent with the yield of xanthan gum under the fermentation condition of 28 DEG C.

The present application belongs to the field of biotechnology, and particularly relates to a kind of production alpha-ketoglutaric acid recombinant escherichia coli engineering strain and its construction method and application.The present application engineering strain A09 can realize high-efficiency catalysis to high-concentration L-glutamic acid sodium without adding any membrane breaker such as Triton X-100.Under the condition of high-intensity catalysis for 6 hours, the cell membrane structure of the bacterial cell is kept intact without obvious damage.This characteristic completely eliminates the problems such as cell toxicity, product purification complexity, cost increase and process safety reduction caused by the use of surfactant in traditional methods, realizes the perfect unity of "cell integrity" and "high catalytic efficiency", and provides an unprecedented technical solution for the large-scale, industrialized and low-cost production of alpha-ketoglutaric acid.

This invention relates to the field of fertilizer technology and discloses a biomass fertilizer for improving the soil environment and its preparation method. The invention uses lignin graft copolymer and modified sodium alginate as raw materials, and prepares a composite hydrogel using the calcium ion in-situ gelation method. Biochar, urea, and potassium dihydrogen phosphate are then added to the hydrogel to obtain a biomass fertilizer for improving the soil environment. The pyrrole groups in the lignin graft copolymer and the nitrogen atoms in the Mannich base structure act as hydrogen bond acceptors, enhancing the hydrogen bonding with the hydrogel molecular chains and improving the water retention capacity of the fertilizer. The rigid structure of lignin itself also plays a role in the gel network, reducing the collapse of the cross-linked network, thereby forming a richer three-dimensional porous structure and enhancing the water retention capacity of the fertilizer. Quaternary ammonium salt groups can disrupt the bacterial cell membrane structure, leading to the extravasation of bacterial substances and enhancing the antibacterial effect of the fertilizer. Thiourea groups can bind to enzymes within bacterial cells, exerting an antibacterial effect.

This invention relates to a method for the simultaneous removal of antibiotic-resistant bacteria and resistance genes using a solar-driven interfacial heat-Fenton oxidation-like process. The method involves placing a photothermal film loaded with carbon black composite photothermal material in a photothermal sterilization reactor, introducing antibiotic-resistant wastewater, adding H2O2, turning on a xenon lamp to preheat the photothermal film, sealing the reactor, and allowing the wastewater to pass through the photothermal film under gravity. This invention utilizes a photothermal conversion material to absorb light radiation and convert it into thermal radiation, treating antibiotic-resistant bacteria through the high-temperature interfacial heat effect generated on the photothermal film. High-temperature interfacial heat causes localized heating of the bacterial cell membrane structure, leading to a certain degree of breakage of fatty acid chains in the bacterial membrane lipid bilayer. This increases cell membrane permeability, and the resulting physical damage damages the bacterial outer membrane structure, further increasing membrane permeability and thus disrupting membrane integrity.

This invention provides a wall-mounted disinfection device, comprising a housing, an air inlet assembly with a primary filter at the lower end of the housing, and an air outlet assembly at the upper end of the housing; a wall-mounting connector located on the back of the housing; a disinfection assembly positioned between the air inlet and outlet assemblies, comprising at least two layers of flexible disinfection mesh made of a flexible, high-temperature resistant material, with nano-disinfecting materials loaded on its surface; a monitoring module positioned between the air inlet and outlet assemblies; and a control module connected to a display panel; and a power supply module including a charging circuit board and an external power interface. The beneficial effect is that by using a flexible disinfection mesh loaded with nano-disinfecting materials as the core disinfection component, the nanomaterials can effectively destroy the cell membrane structure of bacteria and viruses. Combined with an optional ultraviolet-assisted disinfection lamp, synergistic disinfection is achieved, achieving a sterilization rate of up to 99%, and exhibiting excellent purification effects on harmful pollutants such as PM2.5 and formaldehyde in the air.

This invention relates to a multi-effect skincare essence for improving skin function and its preparation method. The invention supplements essential lipids and phospholipid precursors for the skin by adding linoleic acid and ethanolamine, strengthening the stratum corneum barrier and cell membrane structure integrity. It also adds serum albumin to address the poor water solubility and easy oxidation of linoleic acid and ethanolamine, improving the stability and skin affinity of the active ingredients. Simultaneously, it adds stable inorganic salts of essential trace elements such as selenium, silicon, tin, vanadium, manganese, and molybdenum to enhance the skin's anti-aging, repair, and firming abilities. Furthermore, it adds transferrin, which, on the one hand, blocks iron-mediated Fenton reactions and free radical generation, reducing photoaging and oxidative stress damage; on the other hand, transferrin prevents oxidative inactivation of linoleic acid, vitamins, selenium, tin, and other components under iron ion catalysis, reducing oxidative loss and protecting the formula's activity. Thus, it synergistically achieves effects such as barrier repair, anti-inflammatory soothing, anti-aging and firming, and brightening of skin tone.

This invention relates to the field of traditional Chinese medicine preparations, and discloses a topical preparation of black alum for treating malaria and rapidly regulating anemia. It is composed of black alum, cinnabar, artemisia annua extract, dichroa febrifuga extract, angelica sinensis extract, astragalus membranaceus extract, and pharmaceutical excipients. Based on black alum and cinnabar, this invention incorporates artemisia annua extract and dichroa febrifuga extract. Black alum exerts its insecticidal effect by disrupting the cell membrane structure of Plasmodium; cinnabar inhibits nucleic acid synthesis in Plasmodium; artemisinin interferes with mitochondrial function in Plasmodium; and dichroa febrifuga alkaloids inhibit protein synthesis in Plasmodium. The preparation is made into a nasal powder, administered through nasal inhalation. It is directly absorbed into the bloodstream through the rich capillaries of the nasal mucosa, rapidly replenishing the body's iron and promoting hemoglobin synthesis. The added angelica sinensis and astragalus membranaceus extracts have blood-tonifying and qi-boosting effects, enhancing the body's hematopoietic function, improving microcirculation, and accelerating the improvement of anemia symptoms.

This application provides a cell-like membrane structure, its preparation method, and its application. The cell-like membrane structure is a block copolymer, consisting of a hydrophilic end and a hydrophobic end; the hydrophobic end contains unsaturated bonds; the hydrophobic end is cross-linked through these unsaturated bonds to form the cell-like membrane structure. This application significantly enhances the interaction forces between the bilayers by cross-linking the hydrophobic ends of the block copolymer through unsaturated bonds, thereby improving membrane stability; furthermore, the cross-linking immobilizes nanoporous proteins within the membrane, increasing the binding strength between the pores and the membrane and reducing the risk of nanoporous protein detachment.