108 results about "Cell membrane structures" patented technology

The invention relates to a combined drug for treatment of cow hoof disease. The drug comprises ethylenediamine dihydroiodide and copper sulfate that are mixed in a mass ratio of 1:2-2:3. The ethylenediamine dihydroiodide can react with copper sulfate to release free elementary iodine, free state iodine atom has ultrastrong oxidation, and can enable protein denaturation and destroy the cell membrane structure of pathogens, thereby achieving the purposes of preventing infection and treating cow hoof disease. The drug provided by the invention has the advantages of short treatment cycle, high cure rate and no pollution to the environment and milk production.

The invention discloses an imitation mussel attachment protein and cell membrane structure double-bionic copolymer shown by the structural formula (I), wherein m is an integer from 10 to 1,000, n is an integer from 0 to 500, and x is an integer from 5 to 500; in the m, n and x, the molar percentage of m is 30-90%, the molar percentage of n is 0-50%, and the molar percentage of x is 10-70%; R1, R2 and R3 are H or CH3; R4 is an alkyl with 4-18 carbon atoms; V is a catechol group with 2-300 carbon atoms in chain connection; W is a hydrophilic group with 2-8 carbon atoms in chain connection; and the hydrophilic group is a quaternary ammonium group, phosphorylcholine group, pyridinium, sulfonic acid group, phosphate group, carboxylic acid group or sulfuric acid group. According to the invention, the coating is in firm combination, the copolymer is almost suitable for an establishment method of a surface imitation cellulosa membrane structure of any material, and the surface hydrophilic performance and biocompatibility of artificial organ and related materials, medicine controlled-release systems, separation materials and other materials can be improved.

The invention discloses a method for preparing metal reinforced uranium dioxide nuclear fuel pellets. The method mainly comprises two steps: firstly, preparing core-shell structure granules, namely performing low-temperature rapid pre-sintering on UO2 powder by using a Spark Plasma Sintering SPS technique, pelletizing, balling to obtain UO2 pellets, performing physical mixing on the UO2 pellets with metal (one of Mo, Cr, W and the like) micro powder to coat surfaces of the UO2 pellets by the metal micro powder, thereby obtaining metal coated uranium dioxide core-shell structure granules; secondly, preparing a nuclear fuel pellets, namely performing high-temperature liquidation on the metal powder on the surfaces of the UO2 pellets, thereby forming a micro cell structure continuous phase similar to a cytomembrane structure around the UO2 pellets, and obtaining the special metal reinforced UO2 nuclear fuel pellets with a UO2 substrate.

The invention discloses a method for forming a simulated cell outer layer membrane structure on the surface of cross-linked chitosan. The method comprises the following steps that: firstly, the chitosan is cross-linked by glutaraldehyde; and secondly, the surface of the cross-linked chitosan is grafted with phosphinylideyne choline groups to construct the simulated cell membrane structure, the reaction of the grafted phosphinylideyne choline groups is carried out in an anhydrous non-protonic solvent, the reaction temperature is between 40 and 80 DEG C, and the concentration of phosphorylcholine dichloride is between 0.1 and 20mg/ml. The test results show that for the surface modified by the simulated cell outer layer membrane structure, the capabilities of adsorbing protein and blood platelet are obviously reduced, and the blood compatibility is obviously improved. The modified material with the simulated cell outer layer membrane structure has wide application prospect in fields such as blood purification, body implantation materials, tissue engineering, slow release of medicines, biological sensors, and the like.

The present invention relates to the method and equipment for purifying drinking water. Electric field is applied to active carbon fiber filtering material, so that current will flow directly through active carbon fiber filtering material while water to be purified flows through the active carbon fiber filtering material, and under water is thus purified under the action of both electric field and flow field. Owing to the collision between pollutant and bacteria with active carbon fiber and direct current action on bacteria to destroy the cell membrane structure, the said method of the present invention can inhibit the growth of the bacteria and kill bacteria without throwing antiseptic.

The invention discloses a preparation method of instant dried white fungus. The preparation method is characterized in that: dry white fungus is used as a raw material; and the preparation method comprises the production process flows of impurity removal, soaking, balancing, softening, drying and cooling. By adopting the process of soaking in low-concentration ethanol solution for short time and balancing, the white fungus properly absorbs water; by adopting the high-temperature steam softening process, the tough cell membrane structure of the white fungus is effectively destroyed; and by adopting the normal-pressure and low-temperature drying process, the operation is simple. The dried white fungus produced by the method has the characteristics of convenience for carrying and capability of being quickly rehydrated and dispersed after brewing; the obtained white fungus porridge has the same exquisite and smooth mouthfeel as the boiled white fungus porridge; naturally pure form, flavorand color of the white fungus are kept; and the dried white fungus is easily digested and absorbed, and can be transported, stored and sold at normal temperature.

The invention discloses a fluorescent labeling method for living organisms having cell membrane structures. The fluorescent labeling method comprises the following steps: co-culturing choline analogue containing azide group and a living organism having a cell membrane structure and acquiring choline analogue modified living organism after metabolism; and marking the choline analogue modified living organism by adopting DBCO (dibenzocyclooctyne) group modified fluorescent marker through the click chemical reaction and enabling the fluorescent marker to be labeled on the surface of the cell membrane of the living organism. According to the fluorescent labeling method for the living organism having the cell membrane structure, the choline analogue containing azide group is adopted to modify the living organism, and the method has little influence on the activity of the living organism; and the fluorescent labeling method adopts a one-step method for marking, compared with the two-step method, the one-step method can be operated simply and has high efficiency.

The invention discloses a preparation method for ZCHH tablets and a method for reversing the drug tolerance of MRSA (Methicillin-Resistant Staphylococcus Aureus) by using ZCHH and an antibiotic together. The preparation method comprises the following steps: thoroughly mixing ZCHH with lactose according to the weight ratio of the ZCHH to the lactose of 40 to 9; preparing wet granules with starch slurry of 10%; treating the wet granules with a 16-mesh sieve; drying at 60-70 DEG C; after granule straightening, adding magnesium stearate of which the weight is 1% of that the granules to the granules; compressing to form the ZCHH tablets. The method for reversing the drug-tolerance of the MRSA by using the ZCHH and the antibiotic together comprises the following steps: MRSA strains are isolated clinically; only ZCHH as well as both ZCHH and a beta-lactam antibiotic are applied to in vitro bacteriostasis; only ZCHH as well as both ZCHH and a beta-lactam antibiotic are applied to in vivo bacteriostasis; the structural hierarchies of the cell walls and cell membranes of the MRSA bodies are observed; statistical analysis is performed on the in vivo and in vitro antibiosis effects of the ZCHH and the beta-lactam antibiotics which are used together. According to the invention, the dead zone of action mechanism that the drug tolerance of the MRSA is reversed through drug combination is filled up, and an important theoretical basis is provided for clinical medication.

The invention discloses a preparation method of nano micellar solution of polymer with simulated cellulosa membrane structure. The preparation method comprises the following steps: (1) preparing polymer with simulated cell membrane structure with mass ratios of MPC:LMA being 3:7-7:3 and MPC:SMA being 3:7-7:3(2) preparing micellar solution of the polymer; (3) agitating the prepared micellar solution of the polymer at 50-90 DEG C for heat treatment for 5-90min; and (4) cooling and filtering to obtain the nano micellar solution of polymer with simulated cellulosa membrane structure. The invention prepares micellar solution of polymer by simple and convenient direct dissolving method and solvent volatilizing method, and obtains nano micellar solution with compact structure and narrower granulometric distribution after treatment of agitating for 5-90min at 50-90 DEG C.

The invention relates to the field of polymer modification, and in particular to a modified polyethyleneimine and application thereof in the preparation of a gene transfection vector reagent. According to the present invention, hydrophobic rosin acid or dehydroabietic acid is grafted onto polyethyleneimine to obtain the modified polyethyleneimine. The hydrophobic group of the modified polyethyleneimine forms a dense hydrophobic core with strong hydrophobicity due to the intermolecular forces in water, hydrophilic polyethyleneimine forms hydrophilic shell, thereby forming a nano micelles with shell-core structure, and nanoparticles with small particle size formed after DNA binding. The composite nanoparticles in smaller size gains tighter structure, so that composite nanoparticles are easier to pass through the cell membrane structure of a lipid bilayer, and the efficiency of transfection is improved. The modified polyethyleneimine can be applied to the preparation of gene transfection vectors and has the characteristics of high biological safety, high transfection efficiency, and low cost.

The invention relates to a spinach planting method which includes the steps of seed processing, airing, seed soaking, germinating, seedling breeding, thinning and final singling, field managing and harvesting. By means of the method, by processing seeds, the sprouting rate of the spinach seeds can be effectively increased, by applying base fertilizer in the seedling breeding process, the nutrientrequirements of the seedling growth stage can be met, by spraying a botanical insecticide containing various active substances in raw materials in the field managing process, the cell membrane structure and functions of insects can be destroyed, insect death is caused, and the botanical insecticide has high killing capacity to insects, hinders the growth and reproduction of insects, has no side effects on human or animals and has no pollution to the environment. The planted spinach has the advantages of being rich in nutrient, large in yield and healthy to eat.

The invention discloses a biomimetic amphiphilic graft copolymer with a cell membrane structure. The main chain of the copolymer is chitosan, and the side chains are hydrophilic choline phosphate and hydrophobic aliphatic acid. The prepared amphiphilic graft copolymer has the biomimetic characteristics of cell membrane in structure; the choline phosphate molecules mimic the head parts of phospholipid molecules in cell membrane, the sugar units of the chitosan mimic the head parts of the glycolipid molecules in the cell membrane, and the hydrophobic aliphatic acid mimic the hydrophobic carbon chains of the phospholipid molecules and the glycolipid molecules. The copolymer has very good biological compatibility and hydrophilicity. When the copolymer is taken as the nano drug carrier, the copolymer can resist the non-specific adhesion of proteins, and can be easily swollen by the focal cells. Moreover, the graft copolymer can be evenly and stably dispersed in a solution with a high salinity and wide pH range. The invention further provides a preparation method of the copolymer at the same time. The preparation method has the advantages of simple operation, low cost, mild reaction conditions, and high yield.

The invention discloses a paraboloid condensation and hot steam pulsation combined multistage falling-membrane seawater desalination device. The paraboloid condensation and hot steam pulsation combined multistage falling-membrane seawater desalination device comprises a compound parabolic concentrator, a convex transparent material, water distributors, a heat receiver and a semipermeable membrane. Light rays are focused on the heat receiver after entering the compound parabolic concentrator and being subjected to reflection of the inner surface and secondary reflection of the vertical wall face, and with temperature rising continuously, the heat receiver heats seawater. A bottom chamber is similar to a cytomembrane structure, the seawater evaporates continuously, and pressure in the chamber increases to enable bottom hot seawater to be pumped from a guide pipe into the top water distributor by means of pulsation and then form falling-membrane flowing on the upper surface of a convex. Steam close to a lower chamber condensates on the lower surface of the convex, and heat is transferred to a falling-membrane water body on the upper surface to enable the steam to evaporate. The seawater flows down stage by stage under the action of gravity, the concentration of the seawater in the bottom chamber increases, and external seawater is supplied due to the fact that pure water decreases in the chamber under the action of the semipermeable membrane on the bottom side of the chamber. Fresh water tanks disposed on reflection surfaces of evaporation chambers in all layers are used for outputting fresh water.

The invention discloses a method for calcification of cancer cells. The method comprises the following steps: (1) cancer cells are inoculated to a folic acid containing medium, incubation is carried out, and cancer cells with folic acid enriched on the surface are obtained; (2) the cancer cells with folic acid enriched on the surface are placed in calcification liquid for incubation, and calcified cancer cells are prepared. The invention also discloses an application of folic acid to preparation of a cancer cell calcification inducer. The method is based on the characteristic that cancer cells highly express folic acid receptors and folic acid carboxyl provides a nucleation site for calcification, calcium phosphate calcified layer is formed on the surface of the cancer cells, so that cell activity is inhibited, cell membrane structures are broken, and final cancer cell death is leaded to; animal experiments show that the calcification treatment does not influence blood cells and does not have hepatotoxicity, the calcification treatment almost does not damage organs and has good biosecurity, so that the technical scheme provides a feasible scheme for tumor treatment.

The invention discloses a hollow fibrous membrane with a cell membrane simulation structure and a construction method thereof. The method comprises the following steps of: dissolving a terpolymer containing a trimethoxy silicon crosslinkable group, a hydrophilic group and hydrophobic alkyl into an organic solvent; forming a coating on the surface of a hollow fibrous membrane with a spraying or dipping method; and treating the coating in aqueous solution steam of triethylamine, trimethylamine or ammonia for 2-5 days to form a stably-crosslinked cell membrane simulation structural coating. The hollow fibrous membrane used in the invention is a micropore fibrous membrane, so that the surface area of the hollow fibrous membrane is increased, and adsorption of stabilization of a polymer coating are facilitated; and crosslinking fixation is performed on the polymer on the surface of a hollow fibrous membrane material after coating, so that the stability of the coating can be enhanced remarkably, and high hydrophilicity and high biocompatibility are achieved.

The invention discloses a preparation method of soybean milk, belonging to the field of food processing. In the invention, microwave and/or ultrasonic-assisted processing is adopted, and cavitation effect, mechanical action and stronger selectivity and penetrating power of microwave and ultrasonic wave are utilized, so that the structures of plant cells and membranes in the bean dregs are destroyed, the penetrating capability of contents of cells through the membranes is increased, and the extraction efficiency of nutritional ingredients such as proteins is improved. In addition, the physical assisted extraction means can avoid introduction of chemical substances, thereby being safe and reliable. For the soybean milk produced by the method disclosed in the invention, many defects of preparing soybean milk by mixing raw pulp and cooked pulp are overcome, the nutritional ingredients including proteins, fat and total saccharides in the bean dregs are sufficiently extracted and utilized, and the yield of the soybean milk is improved. The produced soybean milk is mellow and delicious without undesirable smell. The preparation method has low requirement on equipment.

The invention discloses a 'leaf margin' physiological disease prevention and control agent for walnuts and belongs to the technical field of nutrition regulation. The prevention and control agent comprises salicylic acid, glycine betaine, calcium nitrate, heteroauxin, fulvic acid and water. The 'leaf margin' physiological disease is caused by the fact that sodions and chloridions accumulate excessively on leaves of the walnuts and harm is caused for cells. The salicylic acid, the glycine betaine and the heteroauxin can regulate ion absorption, the calcium nitrate can prevent sodions from absorbing and can maintain the structure of a cell membrane, the fulvic acid can restrain transpiration and prevents the sodions and the chloridions from being conveyed upwards, all substances are matched, and the harm of the sodions and the chloridions for the walnuts is reduced.

The invention relates to the technical field of preparation of papermaking assistants, and concretely relates to a method for preparing an antibacterial and anticoagulant papermaking wet strength agent. Modified chitosan is self made, and the modified chitosan reacts with modified PAE resin under an acidic condition to obtain the antibacterial and anticoagulant papermaking wet strength agent. Themodified chitosan improves the contact with bacterial negative cell bodies, destroys bacterial cell membranes to cause the leakage of intracellular enzymes and nucleic acids, and destroys the cell membrane structure to cause penetration of potassium ions and ATP, so the growth of bacteria is inhibited in order to achieve the antibacterial purpose; an emulsion increases the tightness between fibers, and the emulsion is combined with the hydroxyl groups of paper fibers through covalent bonds to form waterproof covalent bonds, so the dispersion stability is improved; and a diethanolamine modifiedPAE aqueous emulsion reduces the molecular weight of PAE, and the viscosity of the emulsion decreases, so the papermaking wet strength is not prone to generate gel, thereby the wet strength agent hasa broad prospect in papermaking applications.

The invention provides a plant insecticide and a preparation method thereof. The plant insecticide is prepared from the following raw materials: datura flowers, ginkgo leaves, solanumerianthum, fructusxanthii, radix aconitiagrestis, semen strychni, tageteserecta, nericumindicum, herbascutellariaebarbatae, croton seeds, radix euphorbiae lantu, and radix sophoraeflavescentis. The preparation method comprises: mixing the raw materials and then crushing the mixture into powder of 50 meshes; putting the powder into an ultrasonic extractor; soaking the powder with water for 2 hours; starting the extractor for extraction; filtering an obtained medicine solution by a 300-mesh filtering machine; then putting the filtered medicine solution into a 35 DEG C low-temperature spray dryer; and performing spray drying to obtain powder, thereby obtaining plant insecticide powder. The plant insecticide provided by the invention has the following advantages: the plant insecticide destroys cell membrane structures and functions of an insect body to lead to death of the insect body according to plant alkaloid and active substances contained in a plant, has a good insecticidal effect, has no toxic or side effects on human and livestock, and is pollution-free to the environment.

The invention provides a titanium dioxide solid lipid nanoparticle, a dispersion liquid thereof and a method for preparing the same. The dispersion is prepared from an organic phase and water phase, wherein the organic phase comprises titanium dioxide, a lipid material, an emulsifier and an organic solvent in sun-screening effective quantity; and the mass of the lipid material is 6 to 30 times that of the titanium dioxide, the emulsifier is 3 to 15 times of the lipid material, the mass of the organic solvent is 2 to 20 times of the total mass of the titanium dioxide, the lipid material and the emulsifier, and the volume of the water phase is 2 to 15 times of the organic phase. The titanium dioxide nanoparticle is coated in a carrier colloidal particle drug delivery system with slow release effect, thereby realizing the better slow release effect and prolonging the acting time of the sun-screening agent; and due to the cell membrane-similar structure of the solid lipid nanoparticle, the nanoparticle has the advantage of high physiological compatibility of the skin cells and also improves the effect of the sun-screening agent on the skin.

The invention relates to a composite lipid comprising a cholesterol group with a novel structure and an intermediate, relates to a synthesis method and application thereof to a medicament carrier and research on structure and function of a cell membrane as a functional material. The surface of liposome prepared by the composite lipid has a silicate network structure, so that the stability of the liposome is greatly improved; meanwhile, a covalent bond in a formed liposome double-layer membrane structure is connected with the cholesterol group, so that the stability and the permeability of theliposome can be further adjusted.

The invention belongs to the field of medicines. Danshinolic acid B has functions of inhibition of islet amyloid polypeptide aggregation and treatment of diabetes. The in-vivo islet amyloid polypeptide aggregation can destroy a beta-cell membrane structure and a normal form function of mitochondria to cause beta-cell apoptosis and damage a beta-cell function, thereby being taken as an important nosogenesis of type-2 diabetes. By danshinolic acid B, the aggregation of the islet amyloid polypeptide can be inhibited, and the process of damaging the beta-cell membrane structure and the normal form function of mitochondria during aggregation is changed over to protect the beta-cells, so that danshinolic acid B can be used for preventing and treating the diabetes.

The invention relates to a method for preparing agricultural products, in particular to a method for preparing dehydrated vegetable. The invention solves problems of deterioration, low rehydration rate and nutritional component loss caused by high moisture absorption of dehydrated vegetable processed by the existing unreasonable heated-air drying dehydration method. The method comprises the following steps: selecting raw materials, blanching at temperature of 85-95 DEG C for 20-30min, cutting, cooling, soaking in trehalose solution with mass concentration of 2.5-10% for 20-40min, detearing, drying and baking. By carrying out a large mount of experiment based on original heated-air drying dehydration method, the invention realizes the obvious improvement and adds trehalose solution soaking between cooling and detearing so as to ensure cytomembrane structure integrity and rehydration rate of dehydrated vegetable, thereby improving quality. In addition, blanching time and temperature obtained by control experiment can further prevent nutritional component loss of the vegetables and meet requirements of sterilization and enzyme inactivation.

The invention discloses a cell freezing solution for preparing a dry tumor cell quality control and application of the cell freezing solution. According to a formula, the cell freezing solution is prepared by adding 5-25% of protein, 3-15% of a cell stabilizing agent A, 1-10% of a cell stabilizing agent B and 0.01-0.1% of a bacteriostatic agent into PBS (phosphate buffer solution). The cell freezing solution successfully solves the problems that a cell integrity rate after freeze-drying is very low or active ingredients of cell membrane structural proteins and cell contents cannot be reservedwell in the existing cell freeze-drying technology. A preparation method of the dry tumor cell quality control directly uses human-derived tumor cell strains containing cell membrane structures and contents to prepare the quality control; the quality control is derived from human tumor cells and has the same characteristics as those of similar tumor cells in clinical liquid biopsy detection samples; and the quality control prepared by the method is applicable to the quality control of clinical liquid biopsy and can promote extensive popularization and application of the clinical liquid biopsy.