61 results about "Cell membrane structures" patented technology

The invention belongs to the technical field of medicines, and particularly provides a solid dispersion as well as a preparation method and application thereof. The solid dispersion comprises a carrier material, and further comprises quercetagetin and phospholipid, the mass ratio of the quercetagetin to the phospholipid is 1: (0.3-1.5), and the carrier material comprises one or more of a hydrophilic polymer and a pH-dependent polymer. Wherein the quercetagetin and the phospholipid are combined through an intermolecular force, so that the quercetagetin obtains a lipid material similar to a cell membrane structure, a cell membrane bionic phospholipid compound is formed, the cell membrane bionic phospholipid compound can be naturally compatible with an upper wall cell membrane of a small intestine, and the permeation transmembrane capability of the quercetagetin is better promoted; the quercetagetin is added into the solid dispersion, so that more quercetagetin can enter systemic circulation and lymphatic circulation, and therefore, the solid dispersion can strengthen transmembrane absorption while strengthening dissolution, so that the solid dispersion has high bioavailability.

The invention belongs to the technical field of antiviral drugs, and particularly relates to application of astaxanthin in preparation of an anti-influenza A virus product. In-vitro and in-vivo experiments prove that the astaxanthin can inhibit virus adsorption and internalization and relieve damage of viruses to cell membrane structures and functions, the astaxanthin can inhibit virus invasion and directly reduce virus titer in animal bodies, the research result of the invention expands the efficacy range of the astaxanthin, and the astaxanthin can be used for preparing the medicine for treating animal diseases. The astaxanthin is proved to be capable of preventing and treating infection and diseases caused by viruses for the first time.

The application discloses medical use of gelated cells loaded with sulfonated chitosan in treatment of atherosclerosis, and belongs to the technical field of biological medicine. The gelated cells loaded with sulfonated chitosan take macrophages as host cells, and intracellular cross-linking forms a photocrosslinking hydrogel network composed of methacrylated hyaluronic acid and sulfonated chitosan. The gelated cells completely retain cell membrane structure and membrane surface protein expression, lose the proliferation ability, and have excellent blood lipid selective adsorption capacity and foam cell lipid metabolism regulation capacity. In-vivo and in-vitro experiments prove that the gelated cells can significantly reduce blood lipid levels of atherosclerosis model animals, reduce aortic plaque area, and have good biological safety, thereby providing a new strategy and candidate drug for prevention and treatment of atherosclerosis, and having a good clinical application prospect.

The invention discloses a method for removing diatom through combination of pre-oxidation of sodium hypochlorite and potassium permanganate and coagulative precipitation enhancement, and belongs to the technical field of water treatment. The method comprises the following steps: adding sodium hypochlorite and potassium permanganate into diatom-containing water step by step, and carrying out a combined pre-oxidation reaction for 30-60 minutes; and then adding a coagulant to react, and standing and precipitating for about 60 minutes, so that the diatom cells in the water can be efficiently removed. Aiming at the problems of low diatom oxidation removal rate, poor coagulation effect, filter tank blockage and the like in a conventional water treatment process, straight-chain diatom cell membrane structures are primarily destroyed through sodium hypochlorite, negative electricity groups on cell surfaces are exposed, and a favorable reaction interface is provided for potassium permanganate; in-situ manganese dioxide generated by further adding potassium permanganate can coat cells and neutralize surface charges, and an ideal condition is created for the coagulant to form an adsorption bridge, so that a coagulation interface is remarkably improved, and the removal efficiency of diatom cells is improved.

The invention discloses a method for improving yield of anthocyanin extracted by an enzymic method, according to the method, phospholipase is added and used to act on a cell membrane structure, so that the extraction rate of the anthocyanin and the extraction rate of galactoside in the anthocyanin are obviously improved, and the extraction effect of the anthocyanin is greatly improved through a three-stage enzymolysis system. The total enzymolysis extraction time is within 4 h, and compared with 6-7 h of a traditional enzymatic extraction process, the production period is greatly shortened.

The invention relates to the technical field of specific therapeutic activity of pharmaceutical preparations, in particular to application of Avasimibe in preparation of drugs for treating pulmonary fibrosis. The invention relates to an application of Avasimibe in preparation of a medicine for treating pulmonary fibrosis. The Avasimibe is used for reducing the generation of foam cells by targeting SOAT1 so as to relieve the occurrence and development of pulmonary fibrosis. The invention reveals that Avasimibe can reduce the generation of foam cells by targeting SOAT1 so as to relieve the occurrence and development of pulmonary fibrosis. Meanwhile, it is also elaborated that Avasimibe can restore the steady state of cholesterol and cholesteryl ester, improve the fluidity of a cell membrane structure and restore the lipophagy function, and the invention develops the application of Avasimibe in treatment of pulmonary fibrosis diseases.

The invention relates to the technical field of fluorescent probes, in particular to a cell membrane targeted near-infrared AIE fluorescent probe as well as a preparation method and application of the cell membrane targeted near-infrared AIE fluorescent probe. Dithieno [2, 3-b: 2 ', 3'-d] thiophene is adopted as a bridging structure, a triphenylamine unit is connected with a pyridinium salt unit, and the compound is specially used for targeted cell membrane localization and photodynamic tumor treatment. The near-infrared AIE fluorescent probe disclosed by the invention can be combined with a cell membrane to realize an effect of accurately and rapidly positioning cells; and under light stimulation, I-type / II-type ROS can be generated, the cell membrane structure can be destroyed, the cell membrane vacuolation can be realized, the effective killing of in-vivo and in-vitro tumor cells can be realized, and the method has important significance on tumor treatment.

The invention relates to a quinoxaline derivative containing trimethyl and n-propyl as well as a preparation method and application of the quinoxaline derivative, and the derivative is 1, 6, 7-trimethyl-3-propyl quinoxaline-2 (1H)-ketone and is particularly suitable for inhibiting fusarium oxysporum. The derivative is prepared by an organic synthesis method which comprises the step of carrying out cyclization reaction on N-protected o-phenylenediamine and a carbonyl compound in the presence of trifluoroacetic acid. Experiments show that the compound has a remarkable antibacterial effect on fusarium oxysporum, and the minimum inhibitory concentration is 0.50 mg / mL. The derivatives can destroy the cell membrane structure of fusarium oxysporum and cause leakage of RNA, protein and reducing sugar in cells, so that the growth of thalli is inhibited. The invention provides a theoretical basis and an experimental basis for developing a novel efficient and low-toxicity bacteriostatic agent.

The invention discloses a method for killing bovine herpesvirus by sodium chloride aerosol. The method comprises the following steps: (1) preparing sodium chloride aerosol of which the average particle size is 800nm and the particle size distribution is between 500nm and 8mu m, in the specific preparation process, respectively introducing a saturated sodium chloride aqueous solution and absolute ethyl alcohol into dry airflow of a spray dryer according to a volume ratio of 1: 1, and drying by the spray dryer to prepare the sodium chloride aerosol; and (2) the prepared sodium chloride aerosol is used as a bovine herpes virus killing agent to kill bovine herpes viruses, and in the virus killing process, the dosage is 10-60 mg, and the killing time is 10-30 s. The sodium chloride aerosol has the advantages that the sodium chloride aerosol has special antivirus efficacy in a specific dosage and time range, a high-concentration sodium chloride solution can dehydrate bacterial cells and destroy cell membrane structures of the bacterial cells through osmotic pressure difference to cause growth inhibition or death of bacteria, and especially 100% of bovine herpes viruses (BHV-1) can be killed in a very short time with a small dosage of sodium chloride aerosol.

The invention relates to the technical field of antibacterial composition preparation, in particular to a gynecological antibacterial composition and a preparation method thereof, and the gynecological antibacterial composition comprises the following raw materials: modified radix sophorae flavescentis, angelica sinensis, modified cortex phellodendri, radix paeoniae rubra, ligusticum wallichii, bombyx batryticatus, salvia miltiorrhiza, safflower carthamus, fructus cnidii, gallnut, calamine, pomegranate rind, catechu, rhizoma atractylodis, dragon's blood, fructus kochiae, borneol, alum, dried alum, purpurea sal ammoniac, magnesium stearate and starch. According to the invention, after the modified radix sophorae flavescentis is subjected to ethanol ultrasonic extraction, purification and micronization treatment, the dissolution rate and purity of effective components such as sophocarpidine are improved, the cell membrane structure of gram-negative bacteria such as escherichia coli can be quickly destroyed, and efficient bacteriostasis is realized; the modified cortex phellodendri is subjected to alkali dissolution extraction, acid precipitation purification and refined drying, berberine hydrochloride is efficiently enriched, the purity is improved, and the berberine hydrochloride can kill fungi such as candida albicans more accurately and strongly; and through high-temperature calcination and acetic acid activation, the calamine and the catechu are tightly combined at a microscopic level, so that the inherent convergence, granulation promoting and antibacterial effects of the calamine and the catechu are enhanced.

The invention discloses an oenothera biennis lodging-resistant quality-improving yield-increasing functional fertilizer and preparation and use methods thereof, and belongs to the technical field of agricultural cultivation, the fertilizer comprises an active component A and an active component B. The active component A comprises one or more of methyl jasmonate, boric acid, monopotassium phosphate, gibberellin, water-soluble silicon, MgSO4, calcium chloride and melatonin, and the active component B comprises one or more of methyl jasmonate, boric acid, monopotassium phosphate, gibberellin, water-soluble silicon, MgSO4, calcium chloride and melatonin. The active ingredient B is choline chloride; the content of the active component A accounts for 0.5-15.5% of the total weight of the fertilizer, the biological yield, the economic yield and the oil content of the evening primrose are inspected, and when the active component A is single-component methyl jasmonate, the effect is good; when the active component A is composed of multiple components including jasmonic acid methyl ester, the substances provide nutrient elements for the cell wall of the evening primrose, stabilize the cell membrane structure and play a synergistic promotion role in secondary cell wall lignification, so that the lodging-resistant and disease-resistant characteristics of the evening primrose are improved.

The invention discloses a non-destructive culture medium replacement device and method for microalgae, and relates to the technical field of microbiological treatment. Comprising a frame body, a water storage assembly used for storing pure water and calcium-free seawater is arranged above the frame body, a replacement box is arranged in the middle of the frame body and used for containing a dialysis bag containing algae liquid, and the pure water or the calcium-free seawater can flow in and out at the same flow speed through a pipeline and a flow control valve; a collecting assembly used for receiving water discharged from the replacement box is arranged below the frame body. And two groups of symmetrically arranged transverse frames are fixedly mounted in the middle of the frame body. According to the device, a traditional centrifugal method is replaced by physical isolation and micro-vibration auxiliary replacement of the dialysis bag, a knocking assembly driven by a cam is matched with a spring buffer structure, the movement of liquid in the bag is accelerated, meanwhile, mechanical impact force is controlled within a certain amplitude range, it is ensured that the structure of a microalgae cell membrane is complete, and the survival rate is greatly increased; and a high-activity sample is provided for subsequent calcium signal research.

The utility model provides a kind of mixing device for freeze-drying probiotic production line, it is related to probiotic freeze-drying production device technical field, including mixing barrel, stirring device and distributor, the mixing barrel top portion is provided with top cover, mixing barrel bottom is equipped with discharge gate, stirring device is evenly spaced transversely installed in mixing barrel around mixing barrel axis line, the top cover of distributor is coaxial with the mixing barrel and is installed, the distributor bottom is provided with several groups of material distribution hole corresponding with the direction of stirring device in mixing barrel, the application can solve the problem that traditional liquid needs to be combined with the mode that single-point feeding and high-speed paddle type mechanical stirring are combined to form local concentration too high, mixing is not uniform and high shear force destroys bacterial cell membrane structure, causes the problem of active bacteria survival rate decline.

The present invention discloses a preparation process and application of Enterococcus hirae I DO5 freeze-dried powder, which has the following beneficial effects: (1) the preparation process of Enterococcus hirae I DO5 freeze-dried powder of the present invention is simpler and easier to operate than the existing preparation process of preparing Enterococcus hirae I DO5 into immobilized pellets in the prior art; (2) by preparing Enterococcus hirae I DO5 into the form of freeze-dried powder, the low temperature and low pressure during the freeze-drying process can better retain the physiological activity of the Enterococcus hirae I DO5 strain; the Enterococcus hirae I DO5 freeze-dried powder can be stably stored for a long time, reducing the need for frequent replenishment, and is more suitable for long-term use in breeding scenarios; (3) the Enterococcus hirae I DO5 freeze-dried powder has a good deodorizing effect, and the degradation rate of indole, skatole and ammonia in pig manure wastewater can reach more than 60%; (4) the protective agent solution can effectively improve the surface morphology of the Enterococcus hirae I DO5 freeze-dried powder, enhance the cell's anti-freezing ability, maintain the integrity of the cell membrane structure, and improve the freeze-dried survival rate of Enterococcus hirae I DO5.

The invention relates to a breast milk cold sterilization and freeze-drying method for full-closed treatment and application thereof, and is characterized in that the method comprises the following steps: injecting breast milk into a collection bag, carrying out vacuum sealing treatment, carrying out sterilization treatment by adopting cold sterilization, and carrying out freeze-drying to obtain freeze-dried breast milk; the cold sterilization comprises the steps of putting the collection bag into a closed pressure treatment cabin, and carrying out pressure treatment twice. According to the method disclosed by the invention, cold sterilization is adopted to uniformly transfer pressure to microbial cells in breast milk by utilizing the incompressible characteristic of a food-grade hydraulic medium on the premise of not depending on thermal action and avoiding damage of heat to nutritional ingredients, and efficient sterilization is realized by destroying cell membrane structures, enzyme active sites and genetic materials of microorganisms.

This invention discloses an umbilical cord mesenchymal stem cell membrane for treating hair loss, its preparation method, and its application, belonging to the field of biomedical technology. The preparation method of the umbilical cord mesenchymal stem cell membrane includes the following steps: obtaining human umbilical cord mesenchymal stem cells, digesting, centrifuging, washing, and resuspending them to obtain a resuspended cell solution; subjecting the resuspended cell solution to repeated freeze-thaw cycles to break the cells, obtaining a cell lysate; and subjecting the cell lysate to differential centrifugation to obtain the umbilical cord mesenchymal stem cell membrane. This umbilical cord mesenchymal stem cell membrane retains the natural cell membrane structure and functional proteins, has a negatively charged surface, and exhibits good dispersibility and stability. It can improve the survival rate of dermal papilla cells without introducing live cells and effectively regulate the skin microecology and metabolite balance, demonstrating high safety, good biocompatibility, and excellent hair growth promotion effects.

The application discloses a selenium-based metal polyphenol-based nanoparticle and a preparation method and application thereof, and belongs to the technical field of biological materials. The selenium-based metal polyphenol-based nanoparticle is composed of a selenium-based catalytic center, metal ions and non-enzyme molecular ligands, and a stable nano structure is constructed through coordination of the metal ions and polyphenols. The application also discloses a preparation method of the nano enzyme. The nano enzyme can efficiently remove lipid peroxides and lipid hydroperoxides under the participation of glutathione, and has very low non-specific catalytic activity to the POD pathway. The nanoparticle has good biocompatibility, can effectively reduce lipid peroxidation damage induced by oxidative stress, and can maintain the stability of the structure and function of the cell membrane, and can be used for prevention and treatment of oxidative stress-related diseases, and is especially suitable for diseases with lipid peroxidation and ferroptosis as the main pathological mechanism.

The invention discloses a lactobacillus plantarum bacteriostatic agent and application thereof, and relates to the technical field of microbial engineering. According to the lactobacillus plantarum LP2301 provided by the invention, fermentation liquor of the lactobacillus plantarum LP2301 can be used for effectively inhibiting aquatic pathogenic bacteria streptococcus agalactiae, limiting the growth of the streptococcus agalactiae, reducing the use of antibiotics, ensuring food safety and guaranteeing the health of animals and human beings by secreting metabolites such as bacteriostatic proteins and organic acids. Secondly, the invention provides an application of the lactobacillus plantarum bacteriostatic agent, and the lactobacillus plantarum LP2301 is compounded into the bacteriostatic agent; through multi-target cooperation of the cortex phellodendri extract and the rosmarinic acid, the antibacterial efficiency of single probiotics is remarkably optimized; active ingredients of cortex phellodendri directionally destroy a biological membrane barrier of streptococcus agalactiae and disintegrate an adhesion defense system of pathogenic bacteria; rosmarinic acid blocks a bacterial energy metabolism pathway synchronously, so that pathogens lose proliferative power; and exclusive bacteriocin secreted by the LP2301 accurately breaks down a cell membrane structure, so that fatal killing is realized.

This invention relates to the field of agricultural biotechnology, and in particular to a stress-resistant, antifreeze, and preservative biofilm formulation and its preparation method. The stress-resistant, antifreeze, and preservative biofilm formulation is composed of the following raw materials in parts by weight: 13-28 parts film-forming matrix, 5.5-11.5 parts antifreeze active component, 2.51-6.05 parts stress-resistant active component, 7-14 parts crosslinking and plasticizer, 0.1-0.5 parts natural antibacterial agent, and 55-75 parts deionized water. This invention innovatively achieves a four-fold synergy of physical barrier, active stress resistance, efficient antifreeze, and natural antibacterial properties. The film-forming matrix forms a dense and breathable physical barrier, inhibiting moisture loss; the stress-resistant components construct an active stress-resistant system, enhancing the stress resistance of agricultural products; the antifreeze components achieve efficient antifreeze by inhibiting ice crystal formation and stabilizing cell membrane structure; and the natural antibacterial agent specifically inhibits postharvest pathogens such as Penicillium and Botrytis. The four components work synergistically to comprehensively solve multiple problems during the storage of agricultural products.

The invention relates to the technical field of functional boards, in particular to an antibacterial food-grade board and a preparation method thereof.The antibacterial food-grade board is prepared from, by weight, 100 parts of polypropylene resin, 5-15 parts of a composite additive, 8-12 parts of a functional additive, 0.3-0.8 part of an antioxidant 1010 and 0.5-1.5 parts of calcium stearate. The functional additive is compounded with sulfamic acid and a reaction solution containing epsilon-polylysine, epsilon-polylysine can destroy the completeness of bacterial cell membranes and exerts a long-acting antibacterial effect with other substances in the additive through chemical bonding, and the functional additive is compounded with a fructus cnidii extract by utilizing active ingredients of Chinese prickly ash seeds, so that the antibacterial effect of the functional additive is improved. The antibacterial effect is enhanced by destroying a microbial cell membrane structure, and the antibacterial property and food safety of the plate are effectively improved.

The invention discloses a hepatocyte cryopreservation solution which comprises a first cryopreservation mixed solution, a second cryopreservation mixed solution and fetal calf serum, the first cryopreservation mixed solution is prepared from a Leibovitz's L-15 culture medium, lactobionic acid, raffinose, reduced glutathione, ademetionine, curcumin, bicyclol, ursodesoxycholic acid, silymarin and glycyrrhizic acid; the second cryopreservation mixed solution comprises cane sugar, trehalose, hydroxyethyl starch and polyene phosphatidylcholine. On the other hand, the invention discloses a cryopreservation method and a recovery method of primary mouse hepatocytes based on the hepatocyte cryopreservation liquid. The cryopreservation method comprises the following steps: pre-culturing, re-suspending, slowly dropwise adding DMSO (dimethylsulfoxide) and cooling and cryopreserving step by step. The resuscitation method comprises the steps of rapid unfreezing, centrifugal purification and resuscitation culture. The liver cell cryopreservation liquid and the cryopreservation and resuscitation method can play roles in regulating osmotic pressure, inhibiting ice crystal formation, stabilizing cell membrane structure, resisting oxidation, promoting protein synthesis and the like, and the resuscitated cells are high in survival rate and strong in adherence capability and functional activity.

The application belongs to the technical field of microorganisms, and discloses application of shikimic acid in preparation of antibacterial preparations for inhibiting staphyloxin synthesis. It is found that shikimic acid can inhibit synthesis of staphyloxin in methicillin-resistant Staphylococcus aureus (MRSA), reduce tolerance of the bacteria to oxidative stress, induce accumulation of intracellular active oxygen, and damage cell membrane structure and function, including reducing cell membrane order, inducing membrane depolarization, increasing membrane permeability, and leading to ATP leakage and energy metabolism disorder. A new strategy and preparation direction are provided for clinical treatment of multi-drug resistant Staphylococcus aureus infection, and the application has important theoretical value and application prospect.

The invention discloses construction and application of an engineering strain capable of normally growing at 35 DEG C and realizing high yield of xanthan gum. The method comprises the following steps: by taking xanthomonas campestris XC1 as an initial strain, carrying out ARTP mutagenesis treatment, and screening a mutant strain which can normally grow under the condition of 35 DEG C; the mutant strain is subjected to multiple rounds of superposition transformation, and multiple physiological restrictions under high temperature stress are systematically broken by sequentially strengthening a protein folding system (grpE-ATGA-dnaK), activating outer membrane stress regulation (rpoE), constructing an anti-oxidation barrier (katB) and strengthening a cell membrane structure (polCDE); the finally obtained engineering strain shows the performance far better than that of an initial strain under the condition of 35 DEG C; the biomass of the strain is increased by 207.7% compared with that of an initial strain XC1, and the yield of xanthan gum is increased by 75.5% and reaches 25.8 g / L, which is consistent with the yield of xanthan gum under the fermentation condition of 28 DEG C.

The present application belongs to the field of biotechnology, and particularly relates to a kind of production alpha-ketoglutaric acid recombinant escherichia coli engineering strain and its construction method and application.The present application engineering strain A09 can realize high-efficiency catalysis to high-concentration L-glutamic acid sodium without adding any membrane breaker such as Triton X-100.Under the condition of high-intensity catalysis for 6 hours, the cell membrane structure of the bacterial cell is kept intact without obvious damage.This characteristic completely eliminates the problems such as cell toxicity, product purification complexity, cost increase and process safety reduction caused by the use of surfactant in traditional methods, realizes the perfect unity of "cell integrity" and "high catalytic efficiency", and provides an unprecedented technical solution for the large-scale, industrialized and low-cost production of alpha-ketoglutaric acid.

The invention relates to a sweet potato cold-damage-resistant fresh-keeping method with synchronization of heat treatment and composite coating, which comprises the following steps: (1) selecting and grading raw materials; (2) heat treatment and composite coating: completely immersing disinfected sweet potatoes in a treatment tank filled with a composite coating solution, accurately maintaining the temperature of the solution in a range of 48-50 DEG C, (3) cooling and film-forming drying: immediately transferring the treated sweet potatoes into a forced cold air environment at 15-20 DEG C, and drying; then, drying is performed at the temperature of 25-30 DEG C; and (4) packaging and warehousing. According to the method provided by the invention, the anti-cold-damage fresh-keeping effect is remarkable, after the sweet potatoes are treated in the whole process, the sweet potatoes are refrigerated for 60 days at the temperature of 5 + / -0.5 DEG C, the cold damage index is reduced by 50% or above, the MDA content is remarkably reduced, and the integrity of a cell membrane structure is effectively maintained. In addition, thermophysical sterilization and natural antibacterial components have a synergistic effect, and the rotting rate is controlled within 10%; the formed protective film can effectively inhibit water transpiration, and the weight loss rate is controlled within 5%.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a herbicide-resistant rape gene GSTU8 or a protein coded by the herbicide-resistant rape gene GSTU8 in crop breeding, the herbicide-resistant rape gene GSTU8 closely related to herbicide resistance of rape is obtained through screening, a gene sequence is fused and cloned into a carrier PC1300S, then the carrier PC1300S is transferred into the rape, and herbicide-resistant transgenic rape is obtained. The CAT, POD and SOD activities of the transgenic rape are obviously higher than those of conventional seedlings, and the MDA content of the transgenic rape is obviously lower than that of the conventional seedlings; it is indicated that the transgenic line has higher oxidative stress resistance, ROS can be efficiently removed, oxidative stress damage caused by herbicides can be remarkably relieved, the structural stability of cell membranes is kept, and therefore higher glufosinate-ammonium tolerance is shown; field experiments also show that the transgenic line has glufosinate-ammonium tolerance. The rape herbicide-resistant gene GSTU8 screened by the invention can promote the breeding of a new herbicide-resistant rape variety with proprietary intellectual property rights in China.

The invention belongs to the technical field of biological medicine and cell therapy, and particularly relates to a preparation method of a human-derived nasal mucosa mesenchymal stem cell medicine for treating sepsis, which comprises the following steps: cell separation: taking adult nasal mucosa tissues, cutting into pieces, digesting with 0.25% pancreatin, and terminating digestion with a DF12 culture medium containing 20% serum; primary culture: after the tissue adheres to the wall for one week by using the first-step culture medium, replacing a DF12 culture medium containing 10% of serum, and after the cells climb out of the tissue, centrifugally collecting the cells for primary culture; 3, primary culture: identifying a fourth generation of cell surface marker in primary culture by flow cytometry, the invention reveals the deep mechanism of function enhancement of HuNMSCs by inhibiting cell autophagy, maintaining the integrity of a cell membrane structure and improving the survival rate of a sepsis model through molecular means such as lipid metabonomics and the like for the first time, and the deep mechanism of function enhancement of the HuNMSCs is illustrated by using the HuNMSCs to inhibit cell autophagy, maintain the integrity of a cell membrane structure and improve the survival rate of the sepsis model. The possibility of treating sepsis by HuNMSCs is clarified, a brand new cell treatment strategy is provided for sepsis, and a foundation is laid for subsequent clinical application.

The present application provides a kind of dextran main chain zwitterionic polyamino acid side chain brush polymer and its preparation and application, the dextran main chain zwitterionic polyamino acid side chain brush polymer provided by the present application can be used to prepare antibacterial coating, when the antibacterial coating is contacted with water molecule, the hydration of hydroxyl group of dextran and zwitterionic group of polyamino acid occurs simultaneously, at this time, the hydration layer is formed on the surface of coating to prevent the initial adhesion of bacteria and the non-specific adsorption of protein;When bacteria infect material surface, the cationic group of polyamino acid can destroy the structure of bacterial cell membrane, at this time, the coating shows bactericidal activity.The present application constructs the antibacterial coating based on brush polymer, non-leaching, anti-sticking and bactericidal dual-functional antibacterial coating on the surface of polymer medical device through crosslinking reaction, the preparation process is simple, the equipment demand is low, easy to operate, and the feasibility is high.

The invention relates to the technical field of biological pharmacy, in particular to an immunopotentiator containing high-activity microbial protein peptide and a preparation method of the immunopotentiator. The immunopotentiator containing the high-activity microbial protein peptide comprises the following raw materials: beta-glucan, composite immune peptide, a freeze-drying protective agent, a metal organic framework and the balance of a stabilizer. The preparation method comprises the following steps: firstly, constructing an immune activation core skeleton by using beta-glucan, and activating a phosphorylation pathway by using Kluyveromyces marxianus source peptide in the composite immune peptide; the silkworm chrysalis protein peptide is in targeted combination with a receptor compound, so that the phagocytic rate of macrophages is increased; the recombinant lactoferrin peptide destroys a lipid bilayer of a microbial cell membrane through a cationic structural domain, activates a TLR4 pathway to promote lymphocyte differentiation, and ensures that the systematic immune defense efficacy is improved; specific antibacterial protein contained in the Fusarium brachypodium FXFB001 fermentation active protein extract can directly destroy a pathogen cell membrane structure and activate a TLR2 / 4 pathway to enhance innate immune response at the same time.

The invention discloses an avian influenza virus poisoning agent, virus killing application and a virus killing method. And directly using the sodium chloride aerosol with the average particle size of 800nm and the particle size distribution of 500nm-8mu m as the avian influenza virus poisoning agent. The sodium chloride aerosol has the advantages that the sodium chloride aerosol with the average particle size of 800nm and the particle size distribution of 100nm-8mu m is prepared by combining an anti-solvent, a spray drying method and specific process parameters and is directly used as the avian influenza virus poisoning agent, the sodium chloride aerosol has the virus killing effect in a specific dosage and time range, bacterial cells can be dehydrated by a high-concentration sodium chloride solution through osmotic pressure difference, and the concentration of the sodium chloride solution in the avian influenza virus poisoning agent is reduced. The sodium chloride aerosol is used for killing the H9N2 subtype avian influenza virus, the cell membrane structure of the H9N2 subtype avian influenza virus is damaged, the growth inhibition or death of the bacteria is caused, especially, 100% killing of the H9N2 subtype avian influenza virus can be achieved with a small amount of sodium chloride aerosol and a short time, and the effect is very