122 results about "Cell membrane structures" patented technology

The invention relates to a universal nano biological vesicle preparation method. The preparation method comprises the following steps: carrying out crude extraction on cells from bacteria, fungi, plants or animals to obtain a crude extracting solution; the crude extracting solution is placed in a low-temperature and high-pressure homogenizer to be subjected to low-temperature and high-pressure homogenizing treatment, then the nano vesicles are obtained, the low-temperature and high-pressure homogenizer comprises a high-pressure cavity, a homogenizing valve, a working cavity, a conveying system and a temperature control system, a slit is formed in the homogenizing valve, and the crude extracting solution located in the high-pressure cavity enters the working cavity through the slit of the homogenizing valve, the pressure intensity of the high-pressure cavity is 300-3000 bar, and the working temperature of the high-pressure homogenizer is 0-10 DEG C. The activation energy is reduced by controlling high-pressure and low-temperature environments, so that the original cell membrane structure is broken, the cell wall structure is destroyed, and phospholipid bimolecules are driven to aggregate and self-assemble to form the vesicles. For biological membranes derived from bacteria, fungi, plants and animals, the vesicles can be prepared by the preparation method disclosed by the invention, and the method has universality.

The invention relates to a method for synchronously removing antibiotic drug-resistant bacteria and drug-resistant genes by combining sunlight-driven interface heat and Fenton-like oxidation, which comprises the following steps of: putting a photothermal film loaded with a carbon black composite photothermal material into a photothermal disinfection reactor, introducing antibiotic drug-resistant bacteria wastewater, adding H2O2, turning on a xenon lamp light source, preheating the photothermal film, and carrying out photothermal disinfection on the photothermal film to remove the antibiotic drug-resistant bacteria and the drug-resistant genes. The photothermal disinfection reactor is sealed, the wastewater is treated through the photothermal film under the action of gravity, the photothermal conversion material absorbs optical radiation and converts the optical radiation into thermal radiation, and antibiotic-resistant bacteria are treated through the high-temperature interface heat effect generated on the photothermal film. The high-temperature interface heat can cause local temperature rise of a bacterial cell membrane structure, so that fatty acid chains in a bacterial membrane lipid bilayer are broken to a certain extent, the permeability of the cell membrane is increased, the structure of a bacterial outer membrane is damaged due to caused physical damage, the permeability of the bacterial membrane is increased, and the integrity of the membrane is damaged.

The invention provides thiamethoxam water dispersible granules and a production process thereof, and belongs to the technical field of pesticide preparation. The production process comprises the following steps: modifying methyl methacrylate; preparing an anti-ultraviolet carrier; loading eugenol; and preparing the water dispersible granules. The water dispersible granule is prepared by mixing the uvioresistant carrier loaded with eugenol with thiamethoxam, the dispersing agent, the wetting agent, the disintegrating agent and the binder, when eugenol and thiamethoxam are combined for use, the pest killing effect can be synergistically enhanced, and in addition, eugenol can bypass the resistance mechanism of pests to thiamethoxam, so that the pest killing effect is improved. Even if pests have certain tolerance to neurotoxicity of thiamethoxam, eugenol can still play a role by destroying cell membrane structures and respiratory systems of the pests, so that the resistance of the pests is broken, and the overall insecticidal effect of the prepared water dispersible granule is enhanced.

According to the fruit and vegetable juice processing technology using the low-temperature and ultrahigh-pressure nanometer wall-breaking sterilization technology, the low-temperature and ultrahigh-pressure sterilization technology is used for destroying the cell membrane structure and enzyme activity of microorganisms through hydrostatic pressure under the low-temperature condition, so that heat damage is remarkably reduced; the original flavor and nutrition of the food are reserved to the greatest extent while efficient sterilization is realized, and the shelf life is remarkably prolonged. Although the existing HPP technology can kill microorganisms at low temperature, the sterilization parameters of different food matrixes are lack of targeted optimization, so that the energy consumption is high or the treatment effect is unstable. Through the combined synergistic effect of different pressure values and pressure maintaining time, optimal sterilization parameters of different food matrixes are focused, so that the balance of precise sterilization and quality regulation and control is realized. The combined synergistic effect of different ultrahigh pressure values and pressure maintaining time is designed through the HPP technology, low-temperature physical sterilization is focused, optimal sterilization parameters are obtained through experiments, and balance of precise sterilization and quality regulation and control is achieved.

The invention belongs to the technical field of medicines, and particularly provides a solid dispersion as well as a preparation method and application thereof. The solid dispersion comprises a carrier material, and further comprises quercetagetin and phospholipid, the mass ratio of the quercetagetin to the phospholipid is 1: (0.3-1.5), and the carrier material comprises one or more of a hydrophilic polymer and a pH-dependent polymer. Wherein the quercetagetin and the phospholipid are combined through an intermolecular force, so that the quercetagetin obtains a lipid material similar to a cell membrane structure, a cell membrane bionic phospholipid compound is formed, the cell membrane bionic phospholipid compound can be naturally compatible with an upper wall cell membrane of a small intestine, and the permeation transmembrane capability of the quercetagetin is better promoted; the quercetagetin is added into the solid dispersion, so that more quercetagetin can enter systemic circulation and lymphatic circulation, and therefore, the solid dispersion can strengthen transmembrane absorption while strengthening dissolution, so that the solid dispersion has high bioavailability.

The invention discloses a preparation process and application of enterococcus hirsutei I DO5 freeze-dried powder. The preparation process has the following beneficial effects that (1) compared with the prior art, the preparation process of the enterococcus hirsutei I DO5 freeze-dried powder disclosed by the invention is simpler and easier to operate when being used for preparing enterococcus hirsutei I DO5 into immobilized pellets; (2) the enterococcus hirsutei I DO5 is prepared into freeze-dried powder, and the physiological activity of the enterococcus hirsutei I DO5 strain can be better reserved at low temperature and low pressure in the freeze-drying process; the enterococcus hirsuti I DO5 freeze-dried powder can be stably stored for a long time, so that the requirement of frequent supplement is reduced, and the enterococcus hirsuti I DO5 freeze-dried powder is more suitable for a long-term use breeding scene (3) the enterococcus hirsutei I DO5 freeze-dried powder is good in deodorization effect, and the degradation rates of indole, skatole and ammonia gas in the pig manure wastewater can reach 60% or above; and (4) the surface morphology of the enterococcus hisei I DO5 freeze-dried powder can be effectively improved through the protective agent solution, the freezing resistance of cells is enhanced, the integrity of a cell membrane structure is maintained, and the freeze-drying survival rate of the enterococcus hisei I DO5 is increased.

The invention relates to the technical field of sealants, in particular to a silicone fireproof sealant for buildings. Quaternary ammonium salt modified silicone oil is added in the preparation process of a silicone sealant base adhesive, and a quaternary ammonium salt group is introduced through a ring-opening reaction of epoxy-terminated polyether and trimethylamine hydrochloride, so that a microbial cell membrane structure is destroyed, and broad-spectrum antibiosis is realized; chemical bonding of quaternary ammonium salt groups and a silicone oil main chain avoids the problem of migration failure of a traditional mildew preventive; secondly, the modified aluminum hydroxide can improve the flame retardant property of the sealant, after the aluminum hydroxide is modified by vinyl trimethoxy silane, the surface hydrophobicity of the sealant can be enhanced, the dispersity of the sealant in base glue is improved, the heat absorption efficiency is high during thermal decomposition, and the combustion speed can be delayed, so that the fireproof performance is improved; the modified aluminum hydroxide is combined with the melamine and the modified hollow glass beads for synergism, so that the prepared silicone fireproof sealant has excellent fireproof performance.

The invention relates to a high-performance antibacterial PP plate and a preparation method thereof, and belongs to the technical field of high polymer materials. The high-performance antibacterial PP board comprises the following components in parts by weight: 60-80 parts of PP resin, 1-4 parts of a dispersing agent, 0.5-1.5 parts of a modified antibacterial agent, 2-6 parts of a modified flame retardant and 0.1-0.5 part of an antioxidant, nano-zinc oxide in the modified antibacterial agent is excited to have strong oxidizing property under ultraviolet light, destroys cell membranes, releases zinc ions to be combined with proteins and interferes bacterial enzyme activity, quaternary ammonium salt groups destroy cell membrane structures, and the quaternary ammonium salt groups and chitosan cooperate to destroy cell wall structures and permeate into the interior to inhibit bacterial activity; boron-based components in the modified flame retardant are heated to dehydrate, carbon formation is accelerated, a glassy protective layer is formed, a Si-O-B structure is constructed, incombustible gas is released, heat and oxygen are insulated, and the modified flame retardant and phosphorus-oxygen double bonds are synergistically flame-retardant; the high-performance antibacterial PP board prepared by the invention not only has a good antibacterial effect, but also has an excellent flame-retardant effect.

The invention belongs to the technical field of antiviral drugs, and particularly relates to application of astaxanthin in preparation of an anti-influenza A virus product. In-vitro and in-vivo experiments prove that the astaxanthin can inhibit virus adsorption and internalization and relieve damage of viruses to cell membrane structures and functions, the astaxanthin can inhibit virus invasion and directly reduce virus titer in animal bodies, the research result of the invention expands the efficacy range of the astaxanthin, and the astaxanthin can be used for preparing the medicine for treating animal diseases. The astaxanthin is proved to be capable of preventing and treating infection and diseases caused by viruses for the first time.

The application discloses medical use of gelated cells loaded with sulfonated chitosan in treatment of atherosclerosis, and belongs to the technical field of biological medicine. The gelated cells loaded with sulfonated chitosan take macrophages as host cells, and intracellular cross-linking forms a photocrosslinking hydrogel network composed of methacrylated hyaluronic acid and sulfonated chitosan. The gelated cells completely retain cell membrane structure and membrane surface protein expression, lose the proliferation ability, and have excellent blood lipid selective adsorption capacity and foam cell lipid metabolism regulation capacity. In-vivo and in-vitro experiments prove that the gelated cells can significantly reduce blood lipid levels of atherosclerosis model animals, reduce aortic plaque area, and have good biological safety, thereby providing a new strategy and candidate drug for prevention and treatment of atherosclerosis, and having a good clinical application prospect.

The invention discloses a method for removing diatom through combination of pre-oxidation of sodium hypochlorite and potassium permanganate and coagulative precipitation enhancement, and belongs to the technical field of water treatment. The method comprises the following steps: adding sodium hypochlorite and potassium permanganate into diatom-containing water step by step, and carrying out a combined pre-oxidation reaction for 30-60 minutes; and then adding a coagulant to react, and standing and precipitating for about 60 minutes, so that the diatom cells in the water can be efficiently removed. Aiming at the problems of low diatom oxidation removal rate, poor coagulation effect, filter tank blockage and the like in a conventional water treatment process, straight-chain diatom cell membrane structures are primarily destroyed through sodium hypochlorite, negative electricity groups on cell surfaces are exposed, and a favorable reaction interface is provided for potassium permanganate; in-situ manganese dioxide generated by further adding potassium permanganate can coat cells and neutralize surface charges, and an ideal condition is created for the coagulant to form an adsorption bridge, so that a coagulation interface is remarkably improved, and the removal efficiency of diatom cells is improved.

The invention discloses a method for improving yield of anthocyanin extracted by an enzymic method, according to the method, phospholipase is added and used to act on a cell membrane structure, so that the extraction rate of the anthocyanin and the extraction rate of galactoside in the anthocyanin are obviously improved, and the extraction effect of the anthocyanin is greatly improved through a three-stage enzymolysis system. The total enzymolysis extraction time is within 4 h, and compared with 6-7 h of a traditional enzymatic extraction process, the production period is greatly shortened.

The invention relates to the technical field of specific therapeutic activity of pharmaceutical preparations, in particular to application of Avasimibe in preparation of drugs for treating pulmonary fibrosis. The invention relates to an application of Avasimibe in preparation of a medicine for treating pulmonary fibrosis. The Avasimibe is used for reducing the generation of foam cells by targeting SOAT1 so as to relieve the occurrence and development of pulmonary fibrosis. The invention reveals that Avasimibe can reduce the generation of foam cells by targeting SOAT1 so as to relieve the occurrence and development of pulmonary fibrosis. Meanwhile, it is also elaborated that Avasimibe can restore the steady state of cholesterol and cholesteryl ester, improve the fluidity of a cell membrane structure and restore the lipophagy function, and the invention develops the application of Avasimibe in treatment of pulmonary fibrosis diseases.

Phosphorus is one of major elements necessary for growth and development of plants, and plays a crucial role in physiological processes and metabolic activities of the plants, such as energy metabolism, nucleic acid synthesis, stability of cell membrane structures, signal transduction and the like. The invention provides a wheat phosphorus absorption and utilization transcription factor TraesCS3D01G144700 and an application of a coding gene of the wheat phosphorus absorption and utilization transcription factor TraesCS3D01G144700 in response to low phosphorus stress. The invention provides new gene resources and technical means for improving the growth and yield of wheat under the condition of low-phosphorus soil and improving the phosphorus utilization efficiency and yield of wheat, and plays an important role in realizing the sustainable development of agriculture.

The invention relates to the technical field of fluorescent probes, in particular to a cell membrane targeted near-infrared AIE fluorescent probe as well as a preparation method and application of the cell membrane targeted near-infrared AIE fluorescent probe. Dithieno [2, 3-b: 2 ', 3'-d] thiophene is adopted as a bridging structure, a triphenylamine unit is connected with a pyridinium salt unit, and the compound is specially used for targeted cell membrane localization and photodynamic tumor treatment. The near-infrared AIE fluorescent probe disclosed by the invention can be combined with a cell membrane to realize an effect of accurately and rapidly positioning cells; and under light stimulation, I-type / II-type ROS can be generated, the cell membrane structure can be destroyed, the cell membrane vacuolation can be realized, the effective killing of in-vivo and in-vitro tumor cells can be realized, and the method has important significance on tumor treatment.

The invention relates to a data processing method based on path encryption in bionic data cells and related equipment. Wherein the bionic data cell comprises a cell membrane structural body, a cytoplasm structural body and a cell nucleus structural body, and the method comprises the following steps: obtaining an initial file, segmenting the initial file to obtain a plurality of data blocks, encrypting the data blocks through a first public key, and storing the encrypted data blocks in the cytoplasm structural body; generating a reading path corresponding to the data block, encrypting the reading path of the data block through a second public key to generate corresponding path encrypted data, and storing the path encrypted data to the cell membrane structure body; a second public key and a first private key corresponding to the first public key are added to the cell nucleus structural body to obtain a bionic data cell file corresponding to the initial file, and the second private key corresponding to the second public key is transmitted according to request information of the user. According to the invention, the security in the data transmission process can be effectively improved.

The invention belongs to the technical field of plant disease control, and particularly relates to application of amide compounds or agrochemically acceptable salts thereof in preparation of antifungal drugs. The amide compound or the acceptable salt of the amide compound shows remarkable broad-spectrum antibacterial activity on various plant pathogenic fungi such as rhizoctonia solani, tobacco target leaf spot bacteria and magnaporthe oryzae, and has no visible phytotoxicity symptom on plants in an effective concentration range. The action mechanism research shows that the amide compound or the acceptable salt thereof inhibits the growth of fungi through multiple ways: (1) the biological wall and cell membrane structures of the fungi are destroyed to cause the outflow of contents so as to play a bactericidal role; (2) the physiological function of fungal normal cells is interfered, so as to induce programmed death of fungal cells. The invention provides an important reference way for developing a high-efficiency novel agricultural bactericide.

The invention relates to a quinoxaline derivative containing trimethyl and n-propyl as well as a preparation method and application of the quinoxaline derivative, and the derivative is 1, 6, 7-trimethyl-3-propyl quinoxaline-2 (1H)-ketone and is particularly suitable for inhibiting fusarium oxysporum. The derivative is prepared by an organic synthesis method which comprises the step of carrying out cyclization reaction on N-protected o-phenylenediamine and a carbonyl compound in the presence of trifluoroacetic acid. Experiments show that the compound has a remarkable antibacterial effect on fusarium oxysporum, and the minimum inhibitory concentration is 0.50 mg / mL. The derivatives can destroy the cell membrane structure of fusarium oxysporum and cause leakage of RNA, protein and reducing sugar in cells, so that the growth of thalli is inhibited. The invention provides a theoretical basis and an experimental basis for developing a novel efficient and low-toxicity bacteriostatic agent.

The invention discloses a method for killing bovine herpesvirus by sodium chloride aerosol. The method comprises the following steps: (1) preparing sodium chloride aerosol of which the average particle size is 800nm and the particle size distribution is between 500nm and 8mu m, in the specific preparation process, respectively introducing a saturated sodium chloride aqueous solution and absolute ethyl alcohol into dry airflow of a spray dryer according to a volume ratio of 1: 1, and drying by the spray dryer to prepare the sodium chloride aerosol; and (2) the prepared sodium chloride aerosol is used as a bovine herpes virus killing agent to kill bovine herpes viruses, and in the virus killing process, the dosage is 10-60 mg, and the killing time is 10-30 s. The sodium chloride aerosol has the advantages that the sodium chloride aerosol has special antivirus efficacy in a specific dosage and time range, a high-concentration sodium chloride solution can dehydrate bacterial cells and destroy cell membrane structures of the bacterial cells through osmotic pressure difference to cause growth inhibition or death of bacteria, and especially 100% of bovine herpes viruses (BHV-1) can be killed in a very short time with a small dosage of sodium chloride aerosol.

The invention relates to the technical field of antibacterial composition preparation, in particular to a gynecological antibacterial composition and a preparation method thereof, and the gynecological antibacterial composition comprises the following raw materials: modified radix sophorae flavescentis, angelica sinensis, modified cortex phellodendri, radix paeoniae rubra, ligusticum wallichii, bombyx batryticatus, salvia miltiorrhiza, safflower carthamus, fructus cnidii, gallnut, calamine, pomegranate rind, catechu, rhizoma atractylodis, dragon's blood, fructus kochiae, borneol, alum, dried alum, purpurea sal ammoniac, magnesium stearate and starch. According to the invention, after the modified radix sophorae flavescentis is subjected to ethanol ultrasonic extraction, purification and micronization treatment, the dissolution rate and purity of effective components such as sophocarpidine are improved, the cell membrane structure of gram-negative bacteria such as escherichia coli can be quickly destroyed, and efficient bacteriostasis is realized; the modified cortex phellodendri is subjected to alkali dissolution extraction, acid precipitation purification and refined drying, berberine hydrochloride is efficiently enriched, the purity is improved, and the berberine hydrochloride can kill fungi such as candida albicans more accurately and strongly; and through high-temperature calcination and acetic acid activation, the calamine and the catechu are tightly combined at a microscopic level, so that the inherent convergence, granulation promoting and antibacterial effects of the calamine and the catechu are enhanced.

The invention discloses an oenothera biennis lodging-resistant quality-improving yield-increasing functional fertilizer and preparation and use methods thereof, and belongs to the technical field of agricultural cultivation, the fertilizer comprises an active component A and an active component B. The active component A comprises one or more of methyl jasmonate, boric acid, monopotassium phosphate, gibberellin, water-soluble silicon, MgSO4, calcium chloride and melatonin, and the active component B comprises one or more of methyl jasmonate, boric acid, monopotassium phosphate, gibberellin, water-soluble silicon, MgSO4, calcium chloride and melatonin. The active ingredient B is choline chloride; the content of the active component A accounts for 0.5-15.5% of the total weight of the fertilizer, the biological yield, the economic yield and the oil content of the evening primrose are inspected, and when the active component A is single-component methyl jasmonate, the effect is good; when the active component A is composed of multiple components including jasmonic acid methyl ester, the substances provide nutrient elements for the cell wall of the evening primrose, stabilize the cell membrane structure and play a synergistic promotion role in secondary cell wall lignification, so that the lodging-resistant and disease-resistant characteristics of the evening primrose are improved.

The invention discloses a non-destructive culture medium replacement device and method for microalgae, and relates to the technical field of microbiological treatment. Comprising a frame body, a water storage assembly used for storing pure water and calcium-free seawater is arranged above the frame body, a replacement box is arranged in the middle of the frame body and used for containing a dialysis bag containing algae liquid, and the pure water or the calcium-free seawater can flow in and out at the same flow speed through a pipeline and a flow control valve; a collecting assembly used for receiving water discharged from the replacement box is arranged below the frame body. And two groups of symmetrically arranged transverse frames are fixedly mounted in the middle of the frame body. According to the device, a traditional centrifugal method is replaced by physical isolation and micro-vibration auxiliary replacement of the dialysis bag, a knocking assembly driven by a cam is matched with a spring buffer structure, the movement of liquid in the bag is accelerated, meanwhile, mechanical impact force is controlled within a certain amplitude range, it is ensured that the structure of a microalgae cell membrane is complete, and the survival rate is greatly increased; and a high-activity sample is provided for subsequent calcium signal research.

The invention belongs to the field of medicine application, and particularly relates to disordered protein cocktail extracted from Antarctic nematodes and application of the disordered protein cocktail in cell low-temperature protection. The disordered protein disclosed by the invention can effectively reduce low-temperature damage and improve the survival rate of cells under a low-temperature condition by preventing ice crystal formation and stabilizing a cell membrane structure. Compared with a traditional low-temperature protective agent, the low-temperature protective agent can play a protective role at low concentration, and the toxicity risk to cells is remarkably reduced. Meanwhile, the natural anti-oxidation characteristic is achieved, oxidative stress damage induced by low temperature can be relieved, and therefore cells are further protected. The method is wide in application range, can be applied to low-temperature preservation of various types of cells, tissues and organs, and is suitable for multiple fields of medicine, agriculture, scientific research and the like.

The utility model provides a kind of mixing device for freeze-drying probiotic production line, it is related to probiotic freeze-drying production device technical field, including mixing barrel, stirring device and distributor, the mixing barrel top portion is provided with top cover, mixing barrel bottom is equipped with discharge gate, stirring device is evenly spaced transversely installed in mixing barrel around mixing barrel axis line, the top cover of distributor is coaxial with the mixing barrel and is installed, the distributor bottom is provided with several groups of material distribution hole corresponding with the direction of stirring device in mixing barrel, the application can solve the problem that traditional liquid needs to be combined with the mode that single-point feeding and high-speed paddle type mechanical stirring are combined to form local concentration too high, mixing is not uniform and high shear force destroys bacterial cell membrane structure, causes the problem of active bacteria survival rate decline.

The present invention discloses a preparation process and application of Enterococcus hirae I DO5 freeze-dried powder, which has the following beneficial effects: (1) the preparation process of Enterococcus hirae I DO5 freeze-dried powder of the present invention is simpler and easier to operate than the existing preparation process of preparing Enterococcus hirae I DO5 into immobilized pellets in the prior art; (2) by preparing Enterococcus hirae I DO5 into the form of freeze-dried powder, the low temperature and low pressure during the freeze-drying process can better retain the physiological activity of the Enterococcus hirae I DO5 strain; the Enterococcus hirae I DO5 freeze-dried powder can be stably stored for a long time, reducing the need for frequent replenishment, and is more suitable for long-term use in breeding scenarios; (3) the Enterococcus hirae I DO5 freeze-dried powder has a good deodorizing effect, and the degradation rate of indole, skatole and ammonia in pig manure wastewater can reach more than 60%; (4) the protective agent solution can effectively improve the surface morphology of the Enterococcus hirae I DO5 freeze-dried powder, enhance the cell's anti-freezing ability, maintain the integrity of the cell membrane structure, and improve the freeze-dried survival rate of Enterococcus hirae I DO5.

The invention relates to a breast milk cold sterilization and freeze-drying method for full-closed treatment and application thereof, and is characterized in that the method comprises the following steps: injecting breast milk into a collection bag, carrying out vacuum sealing treatment, carrying out sterilization treatment by adopting cold sterilization, and carrying out freeze-drying to obtain freeze-dried breast milk; the cold sterilization comprises the steps of putting the collection bag into a closed pressure treatment cabin, and carrying out pressure treatment twice. According to the method disclosed by the invention, cold sterilization is adopted to uniformly transfer pressure to microbial cells in breast milk by utilizing the incompressible characteristic of a food-grade hydraulic medium on the premise of not depending on thermal action and avoiding damage of heat to nutritional ingredients, and efficient sterilization is realized by destroying cell membrane structures, enzyme active sites and genetic materials of microorganisms.

The invention relates to detergent essence containing natural acarus-killing antibacterial essential oil and application thereof, and belongs to the technical field of detergent essence. The invention discloses a detergent essence containing natural acarus-killing bacteriostatic essential oil, the detergent essence takes composite natural plant essential oil as a main component, alcohol substances contained in pogostemon cablin essential oil and thyme essential oil are rapidly evaporated to form gaseous molecules, and the gaseous molecules are diffused into acarus, so that the cell membrane structure and permeability of the acarus-killing bacteriostatic essential oil are destroyed; phenolic compounds contained in the essential oil react with amino acid residues, so that the structure of the essential oil is changed, and then protein is destroyed; the eucalyptus essential oil can interfere with the moisture balance state of the mites, so that glands of the mites cannot absorb moisture. The detergent essence prepared by the invention has relatively strong antibacterial, antifungal and insecticidal characteristics, and the natural plant essential oil of each component acts together to enhance the toxic effect on mites so as to achieve the effect of killing mites, so that the detergent essence can be applied to the fields of daily necessities and cosmetics.

This invention discloses an umbilical cord mesenchymal stem cell membrane for treating hair loss, its preparation method, and its application, belonging to the field of biomedical technology. The preparation method of the umbilical cord mesenchymal stem cell membrane includes the following steps: obtaining human umbilical cord mesenchymal stem cells, digesting, centrifuging, washing, and resuspending them to obtain a resuspended cell solution; subjecting the resuspended cell solution to repeated freeze-thaw cycles to break the cells, obtaining a cell lysate; and subjecting the cell lysate to differential centrifugation to obtain the umbilical cord mesenchymal stem cell membrane. This umbilical cord mesenchymal stem cell membrane retains the natural cell membrane structure and functional proteins, has a negatively charged surface, and exhibits good dispersibility and stability. It can improve the survival rate of dermal papilla cells without introducing live cells and effectively regulate the skin microecology and metabolite balance, demonstrating high safety, good biocompatibility, and excellent hair growth promotion effects.

The invention discloses a preparation method and application of a GS-loaded mesenchymal stem cell exosome, and belongs to the technical field of biology. In order to solve the problems of slow onset and long recovery period of a diabetic skin tissue defect healing method in the prior art, the invention provides a preparation method of a GS-loaded mesenchymal stem cell exosome, the yield of the MSCs exosome is induced to be greatly increased through high-frequency ultrasonic stimulation and a microfluidic circulation technology, and the GS-loaded mesenchymal stem cell exosome is prepared. An original cell membrane structure is broken by using an acoustic fluid nano directional coupling technology, phospholipid bimolecules are driven to aggregate and self-assemble to form exosomes which are wrapped in GS, and effective drug delivery and synergistic tissue repair are realized; according to the method, nucleic acid, protein, saccharide, polypeptide and various small molecule compounds can be loaded in the exosome, and certain universality is achieved. The GS-loaded mesenchymal stem cell exosome provided by the invention can be applied to preparation of drugs for promoting healing of skin tissue defects and promoting regeneration of diabetic skin tissues.

The application discloses a selenium-based metal polyphenol-based nanoparticle and a preparation method and application thereof, and belongs to the technical field of biological materials. The selenium-based metal polyphenol-based nanoparticle is composed of a selenium-based catalytic center, metal ions and non-enzyme molecular ligands, and a stable nano structure is constructed through coordination of the metal ions and polyphenols. The application also discloses a preparation method of the nano enzyme. The nano enzyme can efficiently remove lipid peroxides and lipid hydroperoxides under the participation of glutathione, and has very low non-specific catalytic activity to the POD pathway. The nanoparticle has good biocompatibility, can effectively reduce lipid peroxidation damage induced by oxidative stress, and can maintain the stability of the structure and function of the cell membrane, and can be used for prevention and treatment of oxidative stress-related diseases, and is especially suitable for diseases with lipid peroxidation and ferroptosis as the main pathological mechanism.