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Imitation mussel attachment protein and cell membrane structure copolymer and preparation method and application thereof

A mussel adhesion protein and adhesion protein technology, applied in the fields of polymer chemistry and physics, surface science and biomedical materials, can solve the problem of unstable physical adsorption polymer coating, unsatisfactory modification effect, coating process Complicated problems, to achieve the effect of improved surface hydrophilicity and biocompatibility, good biocompatibility, and simple construction method

Active Publication Date: 2013-01-23
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] One of the objectives of the present invention is to provide a double biomimetic copolymer containing catechol and hydrophilic groups that can adhere to the surface of most materials, so as to solve the problems of unstable physical adsorption polymer coating, chemical Reactive fixed coating process is complicated, and the modification effect is not ideal, etc.

Method used

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  • Imitation mussel attachment protein and cell membrane structure copolymer and preparation method and application thereof
  • Imitation mussel attachment protein and cell membrane structure copolymer and preparation method and application thereof
  • Imitation mussel attachment protein and cell membrane structure copolymer and preparation method and application thereof

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preparation example Construction

[0041] The preparation of the double biomimetic polymer PMCA: under the condition of passing nitrogen, the PMPA copolymer is mixed with 1.5 times equivalent of dopamine hydrochloride or dopa and triethylamine (diisopropylethylamine) in anhydrous organic solvent, React in a reaction flask at 50-78°C for 12 hours. The product solution was dialyzed in an aqueous solution of hydrochloric acid with a pH of 3.0-4.0 to remove impurities, and freeze-dried to obtain a dopamine-imitated cell membrane structure copolymer, ie, a double biomimetic copolymer PMCA. The content of hydrophilic units and catechol groups in the double biomimetic copolymer can be controlled by 1 Confirmed by H NMR characterization.

[0042] The above-mentioned double biomimetic copolymer PMCA can be used to prepare a membrane structure coating imitating the outer layer of cells. The following method is used to prepare the imitation cell outer layer membrane structure coating with the double biomimetic copolymer...

Embodiment 1

[0044] Preparation of copolymer P(MPC-MEONP) containing phosphorylcholine and active ester groups: under nitrogen protection, add 100mL absolute ethanol, 2.58g p-nitrophenoxyformylmethylpropene to a 250mL three-necked bottle Acyl polyethylene glycol ester (MEONP, polyethylene glycol carbon atom is 6), 2.95g methacryloyloxyethyl phosphorylcholine (MPC) and azobisisobutyronitrile 0.0522g as polymerization initiator, Reaction at 65°C for 24h. After removing two-thirds of the solvent by evaporation under reduced pressure, precipitation was carried out with diethyl ether 4 times the volume of the solution to obtain a pale yellow solid, namely P(MPC-MEONP). 1 The molar contents of MPC and MEONP components were determined to be 69% and 31% by H NMR.

Embodiment 2

[0046] Preparation of PMPA containing phosphorylcholine, active ester group and hydrophobic group copolymer: under nitrogen protection, add 150mL absolute ethanol, 1.76g p-nitrophenoxyformyl methacrylic acid to a 250mL three-necked bottle Acyl polyethylene glycol ester (MEONP, polyethylene glycol carbon atoms are about 200), 3.98g methacryloyloxyethyl phosphorylcholine (MPC), 1.27g lauryl methacrylate (LMA) With 0.0703 g of azobisisobutyronitrile as a polymerization initiator, react at 70° C. for 24 hours. After removing two-thirds of the solvent by evaporation under reduced pressure, dialyze with a dialysis bag with a molecular weight cut-off of 6000 for 2 days, and freeze-dry the solution in the dialysis bag to obtain light yellow PMPA solid. 1 The molar percentages of MPC, MEONP and LMA in the polymer PMPA were measured by H NMR to be 61%, 18% and 21%, respectively.

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Abstract

The invention discloses an imitation mussel attachment protein and cell membrane structure double-bionic copolymer shown by the structural formula (I), wherein m is an integer from 10 to 1,000, n is an integer from 0 to 500, and x is an integer from 5 to 500; in the m, n and x, the molar percentage of m is 30-90%, the molar percentage of n is 0-50%, and the molar percentage of x is 10-70%; R1, R2 and R3 are H or CH3; R4 is an alkyl with 4-18 carbon atoms; V is a catechol group with 2-300 carbon atoms in chain connection; W is a hydrophilic group with 2-8 carbon atoms in chain connection; and the hydrophilic group is a quaternary ammonium group, phosphorylcholine group, pyridinium, sulfonic acid group, phosphate group, carboxylic acid group or sulfuric acid group. According to the invention, the coating is in firm combination, the copolymer is almost suitable for an establishment method of a surface imitation cellulosa membrane structure of any material, and the surface hydrophilic performance and biocompatibility of artificial organ and related materials, medicine controlled-release systems, separation materials and other materials can be improved.

Description

technical field [0001] The invention relates to mussel adhesion protein and cell membrane structure double biomimetic copolymer and its preparation method, in particular to an adhesive type (meth)acrylic ester copolymer with imitation cell membrane structure, which belongs to polymer chemistry and physics, surface science and biomedicine field of materials technology. Background technique [0002] Phosphorylcholine is a hydrophilic group of the outer membrane of biological cells. The modified material is coated with a hydrophilic-lipophilic amphiphilic polymer containing phosphorylcholine groups. When it contacts with water, its surface forms an imitation The cell membrane structure will be regarded as self by the cells, which can obtain excellent biocompatibility. [0003] This kind of phosphorylcholine group-containing polymer coating can be attached to the surface of the modified material through physical adsorption or chemical bonding. However, the amphiphilic polymer ...

Claims

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Application Information

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IPC IPC(8): C08F220/36C08F220/18C08F220/38C08F8/00
Inventor 宫永宽刘丽平党媛史素青
Owner NORTHWEST UNIV
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