Supercharge Your Innovation With Domain-Expert AI Agents!

Kit for detecting novel coronavirus neutralizing antibody as well as preparation method and application thereof

A coronavirus and kit technology, applied in the biomedical field, can solve expensive problems, achieve the effects of simplifying operations, speeding up detection time, and reducing costs

Active Publication Date: 2021-06-29
BEIJING BOHUI INNOVATION TECH
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The test is not only expensive, poses a biosafety risk, but is also slow, typically taking 3-5 days to get results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] This embodiment provides a test kit for detecting neutralizing antibodies against novel coronavirus, including an ELISA plate, on which the novel coronavirus 2019-nCov capsid protein is immobilized by physical adsorption; the ELISA plate The new coronavirus 2019-nCov capsid protein specifically binds to the receptor protein ACE-2 labeled with horseradish peroxidase. Specifically, the preparation method of the kit includes the following steps:

[0027] S1. Mix the carbonate buffer (pH=9.6) and the capsid protein of the new coronavirus 2019-nCov to prepare a coating solution with a concentration of 100ng / mL.

[0028] S2. Add the coating solution to the microtiter plate according to the amount of 100 μL per well, and place it at 4°C for coating treatment for 18 hours, and then wash it with normal saline for 4 times. The dosage of normal saline is 250 μl each time. / well, after washing, the participating liquid should be patted dry on a towel, and the enzyme plate with the...

Embodiment 2

[0034] This embodiment provides a test kit for detecting neutralizing antibodies against novel coronavirus, including an ELISA plate, on which the novel coronavirus 2019-nCov capsid protein is immobilized by physical adsorption; the ELISA plate The new coronavirus 2019-nCov capsid protein specifically binds to the receptor protein ACE-2 labeled with horseradish peroxidase. Specifically, the preparation method of the kit includes the following steps:

[0035] S1. Mix the carbonate buffer (pH=9.6) and the capsid protein of the new coronavirus 2019-nCov to prepare a coating solution with a concentration of 80ng / mL.

[0036] S2. Add the coating solution to the microtiter plate according to the amount of 100 μL per well, and place it at 3°C ​​for coating treatment for 16 hours, and then wash it with normal saline for 3 times. The amount of normal saline is 250 μl each time. / well, after washing, the participating liquid should be patted dry on a towel, and the enzyme plate with th...

Embodiment 3

[0042] This embodiment provides a test kit for detecting neutralizing antibodies against novel coronavirus, including an ELISA plate, on which the novel coronavirus 2019-nCov capsid protein is immobilized by physical adsorption; the ELISA plate The new coronavirus 2019-nCov capsid protein specifically binds to the receptor protein ACE-2 labeled with horseradish peroxidase. Specifically, the preparation method of the kit includes the following steps:

[0043] S1. Mix the carbonate buffer solution (pH=9.6) and the capsid protein of the new coronavirus 2019-nCov to prepare a coating solution with a concentration of 120ng / mL.

[0044] S2. Add the coating solution to the microtiter plate according to the amount of 100 μL per well, and place it at 5°C for coating treatment for 20 hours, then wash it with normal saline for 5 times, and the dosage of normal saline is 250 μl each time / well, after washing, the participating liquid should be patted dry on a towel, and the enzyme plate ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention is applicable to the technical field of biomedicine, and provides a kit for detecting a novel coronavirus neutralizing antibody as well as a preparation method and application thereof, the kit for detecting the novel coronavirus neutralizing antibody comprises an elisa plate, and a novel coronavirus 2019-nCov capsid protein is fixed on the elisa plate; and the capsid protein of the new coronavirus 2019-nCov on the elisa plate is specifically combined a receptor protein ACE-2 labeled by horse radish peroxidase. The novel coronavirus 2019-nCov capsid protein is fixed on the elisa plate through a physical adsorption method, and then the novel coronavirus 2019-nCov capsid protein fixed on the elisa plate is specifically combined with receptor protein ACE-2 labeled by horse radish peroxidase to form a ligand compound, so that the effect of protecting the activity of the enzyme-labeled protein is enhanced; meanwhile, the dosage of enzyme-labeled protein is also reduced, and the operation steps and the detection time are reduced.

Description

technical field [0001] The invention belongs to the field of biomedical technology, and in particular relates to a kit for detecting neutralizing antibodies against novel coronavirus and its preparation method and application. Background technique [0002] The new coronavirus (2019-nCov virus) seriously threatens human life and health, and neutralizing antibodies can play a protective role. Simple and rapid detection means and methods are effective methods for diagnosis, treatment, prognosis, and vaccine effect observation. [0003] The 2019 novel coronavirus (SARS-CoV-2, 2019-nCoV) is a subtype of the genus β in the Coronaviridae family, and is a linear single-stranded positive-sense RNA virus with an envelope. 2019-nCoV has several structural proteins, among which the spike protein (S) contains a receptor-binding domain (RBD) responsible for recognizing the cell surface receptor angiotensin-converting enzyme 2 (ACE2). The RBD protein interacts strongly with the human ACE...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/569G01N33/58G01N33/543G01N33/535G01N33/531
CPCG01N33/6854G01N33/56983G01N33/581G01N33/54393G01N33/535G01N33/531G01N2333/165G01N2469/20
Inventor 王志新曾滨
Owner BEIJING BOHUI INNOVATION TECH
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com