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Application of etoposide in promoting bone regeneration and repair

A technology of etoposide and uses, which can be used in medical preparations containing active ingredients, microorganisms, bone diseases, etc., and can solve problems such as nonunion of fractures, mechanical complications, and infections.

Pending Publication Date: 2021-07-02
XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods often lead to problems such as nonunion, infection, mechanical complications, etc.

Method used

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  • Application of etoposide in promoting bone regeneration and repair
  • Application of etoposide in promoting bone regeneration and repair
  • Application of etoposide in promoting bone regeneration and repair

Examples

Experimental program
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Embodiment Construction

[0065] In this example, etoposide was co-cultured with MSCs to determine its working concentration; primary mesenchymal stem cells (MSCs) and osteoblast cell line UMR-106 were treated with etoposide, and the alkaline phosphatase (ALP), alizarin red staining and real-time fluorescent quantitative PCR to observe the effect of etoposide on osteogenic differentiation of stem cells; after co-incubating etoposide and UMR-106 cells, the samples were subjected to transcriptome sequencing and bioinformatics analysis, looking for Etoposide promotes the target genes of osteogenic differentiation of stem cells, and it is verified by Western blot.

[0066] The results showed that etoposide significantly inhibited the proliferation of MSCs when the concentration of etoposide was ≥0.1953 μM; the results of ALP staining, alizarin red staining and real-time PCR showed that etoposide could significantly promote the osteogenic differentiation of stem cells, and the results of RNA sequencing analy...

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PUM

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Abstract

The invention provides application of an active substance. The application is characterized in that the active substance is etoposide or a stereoisomer, a geometric isomer, a tautomer, a racemate, a hydrate, a solvate, a metabolite and a pharmaceutically acceptable salt or prodrug of the etoposide. The application is to prepare a medicine for promoting stem cell osteogenic differentiation and bone regeneration and repair.

Description

technical field [0001] The invention provides a new application of active substances, and specifically relates to the action and mechanism of the antineoplastic drug etoposide in promoting osteogenic differentiation of stem cells and bone regeneration and repair. Background technique [0002] Primary bone tumors include benign tumors and malignant tumors. Osteoma and osteosarcoma are common in benign tumors, while osteosarcoma is particularly common among malignant tumors, mostly occurring in adolescents. The clinical treatment for malignant bone tumors and huge benign tumors is mainly surgery combined with neoadjuvant chemotherapy. Bone tumor surgery usually strives to be thorough to avoid recurrence or malignant transformation. For postoperative huge bone defects, although different techniques can be used for bone reconstruction in clinical work, including allogeneic (species) bone transplantation, internal prosthesis, etc. However, these methods often lead to nonunion, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61K45/06A61P35/00A61P19/08C12N5/077C12N5/0775
CPCA61K31/7048A61K45/06A61P35/00A61P19/08C12N5/0654C12N2501/06C12N2506/1346A61K2300/00
Inventor 张晓玲王传东陆艳青
Owner XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE