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DPA solution relative fluorescence emission intensity standard substance and its preparation method and application

A technology of emission intensity and standard substance, applied in fluorescence/phosphorescence, material excitation analysis, material analysis by optical means, etc., to achieve the effect of stable measurement method

Active Publication Date: 2022-02-22
广东省计量科学研究院(华南国家计量测试中心)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently there is no standard substance for coumarin or other types of fluorescent dyes

Method used

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  • DPA solution relative fluorescence emission intensity standard substance and its preparation method and application
  • DPA solution relative fluorescence emission intensity standard substance and its preparation method and application
  • DPA solution relative fluorescence emission intensity standard substance and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The development of embodiment 1DPA o-xylene solution relative fluorescence emission intensity standard substance

[0069] 1. Standard material sample preparation

[0070] 1.1 Raw materials and reagents

[0071] Raw materials: Commercially purchased high-purity ethyl 6-[4-(diphenylamino)phenyl]coumarin-3-carboxylate, purchased from TCI Chemical Industry Development Co., Ltd. (TCI), 1 bottle.

[0072] Solvent: chromatographically pure o-xylene (CAS: 95-47-6), purchased from Fisher Co., Ltd., in a 4 L package.

[0073] 1.2 Equipment

[0074] 1.2.1 Equipment for standard substance preparation: 2000mL grade A volumetric flask; balance with accuracy of 1 / 100,000 (Sartorius, Germany); electric pipette (Eppendorf, Germany); class 100 clean bench, etc. Passed the test.

[0075] 1.2.2 Standard substance packaging equipment: 5mL brown ampoule bottle and ampoule melting and sealing machine and other equipment.

[0076] 1.2.3 Equipment for standard substance analysis:

[0077]...

Embodiment 2

[0284] Embodiment 2 DPA o-xylene solution relative fluorescence emission intensity standard substance is aimed at the use result of different instrument parts

[0285] Referring to the standard material development process of NIST’s SRM2944, fluorescent standard materials should also be considered when using ① the position of the incident excitation light irradiated on the sample changes, resulting in uncertainty introduced by changes in absorption and inner filter effects; ② the incident and exit grating slits Uncertainty introduced; ③Uncertainty introduced by instrument components such as light sources, reflectors, and detectors; ④Uncertainty introduced due to light anisotropy and polarization of standard substances.

[0286] 1.1 The position change of incident light and the influence of light anisotropy

[0287] When investigating the first and fourth factors, since the DPA o-xylene solution is uniform relative to the fluorescent emission intensity standard substance soluti...

Embodiment 3

[0296] Example 3 Similar Standard Substance Quantity Comparison Verification

[0297] Use respectively:

[0298] 1. The SRM2941 standard substance of NIST (the selection reason is because the scope of its emission wavelength is basically consistent with the DPA o-xylene solution relative fluorescence emission intensity standard substance, and the size is the same during measurement) and the relative fluorescence of the DPA o-xylene solution prepared in Example 1 Emission intensity standard substances were compared for analysis.

[0299] 2. GBW (E) 130100 quinine sulfate fluorescent standard substance (the selection reason is because the scope of its emission wavelength overlaps with three kinds of relative fluorescence emission intensity standard substances, and both are in liquid form) and the DPA ortho-2 prepared in Example 1 The toluene solution was compared and analyzed relative to the fluorescent emission intensity standard substance.

[0300] Use DPA o-xylene solution re...

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Abstract

The invention discloses a DPA solution relative fluorescence emission intensity standard substance, a preparation method and application thereof. The DPA solution relative fluorescence emission intensity standard substance provided by the present invention can be provided under the excitation of 380-450nm, the emission wavelength coverage is 400-830nm, and the wavelength interval is the standard relative fluorescence emission intensity value of 1nm, which can well meet the requirements of related instruments. Calibration requirements for optical and near-infrared bands. The DPA solution relative fluorescence emission intensity standard substance provided by the present invention is mainly as the standard of value transfer, meets the needs of current research and detection, and can be used for fluorescence instruments (such as spectrometer, spectrophotometer, microplate reader, gene amplification instrument, Immunoanalyzer, etc.), calibration of detection modules, evaluation of analytical methods, and quality control in biology, food, pharmaceuticals, chemical products, and cosmetics.

Description

technical field [0001] The invention belongs to the technical field of molecular fluorescence analysis and measurement, and in particular relates to a DPA solution relative fluorescence emission intensity standard substance and its preparation method and application. Background technique [0002] In the field of molecular fluorescence analysis and measurement, the most commonly used in China are GBW(E)130290 liquid phase analyzer verification solution standard substance (naphthalene-methanol solution) and GBW(E)130100 quinine sulfate fluorescent standard substance. Among them, the naphthalene methanol solution can only calibrate one excitation wavelength and its corresponding emission wavelength for wavelength calibration. While GBW (E) 130100 quinine sulfate fluorescent standard substance is excited by visible light at 350nm, the maximum luminous position of the sulfuric acid solution of quinine sulfate is at the 450nm of blue-green (relative emission intensity standard val...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/643G01N21/01
Inventor 尹强黄彦捷罗思婷周瑾艳陈玲廖泽荣
Owner 广东省计量科学研究院(华南国家计量测试中心)
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