Rice seedling culture solution added with exogenous salicylic acid and application of rice seedling culture solution

A technology of rice nutrient solution and salicylic acid, which is applied in the field of heavy metal pollution remediation in farmland soil, and achieves the effects of increasing the distribution ratio of Cd, promoting deposition, and reducing absorption and accumulation.

Active Publication Date: 2021-07-13
CHINA NAT RICE RES INST
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, whether salicylic acid adsorbs and fixes cadmium toxicity by co-regulating the synthesis of pectin and lignin in the root cell wall remains to be further studied.

Method used

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  • Rice seedling culture solution added with exogenous salicylic acid and application of rice seedling culture solution
  • Rice seedling culture solution added with exogenous salicylic acid and application of rice seedling culture solution
  • Rice seedling culture solution added with exogenous salicylic acid and application of rice seedling culture solution

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Embodiment

[0033] The present invention adopts hydroponic experiment, and artificial climate box is cultivated after 3 weeks, harvests the sample and measures the Cd content of paddy seedling root and aerial part respectively, and analyzes the distribution of Cd in root system subcellular component and cell wall component, adopts microplate reader Determination of pectin and lignin synthesis-related enzyme activities and gene expression in the cell wall of rice seedling roots by real-time quantitative PCR.

[0034] In this hydroponic test, the method for reducing the cadmium content in rice seedling roots and shoots, the specific steps are as follows:

[0035] (1) The rice variety tested in the present invention is "Yangdao No. 6". Rice seeds with plump grains were selected and sterilized with 2.5% NaClO for 20 min, then washed with deionized water for 5 to 6 times;

[0036] (2) Put the sterilized rice seeds on wet gauze to germinate, transfer to a floating plate floating in 1 / 2 rice nut...

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Abstract

The invention discloses a rice seedling culture solution added with exogenous salicylic acid and application of the rice seedling culture solution. According to the rice seedling culture solution added with the exogenous salicylic acid, the exogenous salicylic acid is added on the basis of a rice nutrient solution formula, and the addition amount is 100 [mu]mol/L<-1>. The cadmium content of root systems and overground parts of rice seedlings is reduced; the rice seedling culture solution is used for regulating pectin synthesis, deesterification and lignin synthesis to reduce cadmium accumulation of rice plants and relieve poison of cadmium stress to rice seedlings; the rice seedlings are cultured in the rice seedling culture solution for 10 days, the pectin content and deesterification degree of roots are increased, free carboxyl in pectin is increased, and the adsorption and combination capacity of cell walls to cadmium is enhanced; meanwhile, root lignin content is increased, the cell walls are thickened, and cadmium ions are prevented from entering cells. The invention discloses and verifies an important rice Cd regulation mechanism, and provides theoretical guidance and a feasible scheme for safe production of cadmium-polluted rice field soil.

Description

technical field [0001] The invention belongs to the field of remediation of heavy metal pollution in farmland soil, and relates to a culture solution for reducing cadmium content in rice seedling roots and aerial parts and its application. Background technique [0002] Cadmium (Cd) is a highly toxic trace heavy metal that widely exists in nature. In recent years, with the smelting of heavy metal ores in my country, the long-term application of cadmium-containing phosphate fertilizers, and the deposition of industrial "three wastes" and other human factors, the problem of cadmium pollution in farmland has become increasingly prominent. According to the "National Soil Pollution Survey Bulletin" in 2014, the rate of cadmium in my country's soil exceeded the standard by 7%, ranking first among the eight excessive metal elements. Rice is the most important food crop in my country, and it is also a crop with relatively high accumulation of cadmium. Incidents of excessive cadmium ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G5/20
CPCC05G3/00C05G5/20C05B7/00C05C1/00C05C3/00C05D1/02C05D3/00C05D5/00C05D9/02
Inventor 曹珍珍潘九月关美艳陈铭学
Owner CHINA NAT RICE RES INST
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