Rice seedling culture solution added with exogenous salicylic acid and application of rice seedling culture solution
A technology of rice nutrient solution and salicylic acid, which is applied in the field of heavy metal pollution remediation in farmland soil, and achieves the effects of increasing the distribution ratio of Cd, promoting deposition, and reducing absorption and accumulation.
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[0033] The present invention adopts hydroponic experiment, and artificial climate box is cultivated after 3 weeks, harvests the sample and measures the Cd content of paddy seedling root and aerial part respectively, and analyzes the distribution of Cd in root system subcellular component and cell wall component, adopts microplate reader Determination of pectin and lignin synthesis-related enzyme activities and gene expression in the cell wall of rice seedling roots by real-time quantitative PCR.
[0034] In this hydroponic test, the method for reducing the cadmium content in rice seedling roots and shoots, the specific steps are as follows:
[0035] (1) The rice variety tested in the present invention is "Yangdao No. 6". Rice seeds with plump grains were selected and sterilized with 2.5% NaClO for 20 min, then washed with deionized water for 5 to 6 times;
[0036] (2) Put the sterilized rice seeds on wet gauze to germinate, transfer to a floating plate floating in 1 / 2 rice nut...
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