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Method for separating various Siraitic acid monomers from momordica grosvenori roots by continuous method

A kind of Momordica grosvenori, continuous method technology, applied in the field of separation of natural triterpenoid acid monomers, can solve the problem of continuously obtaining multiple kinds of high-content Momordica grosvenori monomers without disclosure, and achieves process continuity, improved yield, and high yield. Effect

Active Publication Date: 2021-07-16
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The above-mentioned prior art does not disclose a method for simultaneously and continuously obtaining multiple high-content mogrosinic acid monomers from the roots of Luo Han Guo

Method used

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  • Method for separating various Siraitic acid monomers from momordica grosvenori roots by continuous method
  • Method for separating various Siraitic acid monomers from momordica grosvenori roots by continuous method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] (1) Enzymolysis: Take 10kg of dried Luo Han Guo root, grind it to a particle size of 2-3mm, add 10kg of hydrochloric acid aqueous solution (pH=5), 0.02kg of sodium borate and 0.1kg of β-glucosidase, stir evenly, and enzymatically Solution 24 hours;

[0055] (2) Alkaline alcohol percolation: put the raw material after enzymolysis into the percolator, use 80L alkaline methanol (the volume percentage concentration of methanol is 70%, containing 1% sodium hydroxide) percolation extraction, percolation The flow rate is 1.0BV / hour, and percolate is obtained;

[0056] (3) ceramic membrane filtration: the percolation liquid is filtered with a 50nm ceramic membrane (filter pressure 0.4Mpa) with an aperture to obtain a ceramic membrane filtrate;

[0057] (4) Decolorization: Pass the ceramic membrane filtrate through an alumina chromatography column (the amount of alumina is 3 kg, the height-to-diameter ratio of the chromatography column is 6:1, and the column flow rate is 1.5 BV...

Embodiment 2

[0064] (1) Enzymolysis: Take 10kg of dried Luo Han Guo root, grind it to a particle size of 2-3mm, add 15kg of dilute hydrochloric acid aqueous solution (pH=6), 0.05kg of sodium borate and 0.2kg of β-glucosidase, stir well, and put it at room temperature Enzymolysis for 18 hours;

[0065] (2) Alkaline alcohol percolation: put the raw material after enzymolysis into the percolator, use 100L alkaline ethanol (the volume percentage concentration of ethanol is 80%, wherein contains potassium hydroxide 0.8%) percolation extraction, percolation The flow rate is 1.5BV / hour, and percolate is obtained;

[0066] (3) ceramic membrane filtration: the percolation liquid is filtered with a 50nm ceramic membrane (filter pressure 0.4Mpa) with an aperture to obtain a ceramic membrane filtrate;

[0067] (4) Decolorization: pass the ceramic membrane filtrate through an alumina chromatography column (the amount of alumina is 2 kg, the height-to-diameter ratio of the chromatography column is 7:1,...

Embodiment 3

[0074] (1) Enzymolysis: Take 10 kg of dried Luo Han Guo root, grind it to a particle size of 2-3 mm, add 15 kg of dilute sulfuric acid aqueous solution (pH=5), 0.03 kg of sodium borate and 0.15 kg of β-glucosidase, stir evenly, and bring to room temperature Enzymolysis for 20 hours;

[0075] (2) Alkaline alcohol percolation: put the raw material after enzymolysis into the percolator, use 90L alkaline methanol (the volume percent concentration of methanol is 85%, containing 1.5% sodium hydroxide) percolation extraction, percolation The flow rate is 0.8BV / hour, to obtain percolate;

[0076] (3) Ceramic membrane filtration: filter the percolation liquid with a pore size of 100nm ceramic membrane (filter pressure 0.2Mpa) to obtain ceramic membrane filtrate;

[0077] (4) Decolorization: pass the ceramic membrane filtrate through a magnesia chromatography column (the consumption of magnesia is 2.6kg, the height-to-diameter ratio of the chromatographic column is 8:1, and the column ...

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Abstract

The invention relates to a method for separating a plurality of Siraitic acid monomers from momordica grosvenori roots by a continuous method, which comprises the following steps: carrying out enzymolysis on the momordica grosvenori roots under a weakly acidic condition, carrying out percolation extraction, filtration, decoloration and concentration by using an alcohol-water solution under an alkaline condition, and extracting the obtained concentrated solution under an acidic condition to obtain total Siraitic acid; then carrying out gradient elution separation on the momordica grosvenori total acid through chromatography, and the total Siraitic acid sequentially comprises Siraitic acid B, Siraitic acid A, Siraitic acid D and Siraitic acid C according to the polar outflow sequence. According to the invention, percolation extraction is carried out after enzymolysis under an alkaline condition, and the mogroside is hydrolyzed to form free Siraitic acid, salified and leached, so that the yield of the Siraitic acid is improved. The method has the advantages of coherent and simple process, strong operability, low production cost, no use of toxic and harmful chemical solvents, safety, environmental protection, high product content and high yield. The invention provides a method for extracting and separating total Siraitic acid from siraitia grosvenorii roots, which is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for separating natural triterpene acid monomers, in particular to a method for continuously separating various mogroscarpic acid monomers from the roots of Luo Han Guo. Background technique [0002] Luo Han Guo is a rare local specialty in Guilin, mainly produced in Yongfu County and Longsheng County, and it is also one of the first batch of "dual-use medicine and food" materials approved by the state. Mogroside is a unique natural high-power sweetener in Luo Han Guo. Its sweetness is 300 times that of sucrose, and its calories are zero. preventive effect. As a food, mogroside is safe and non-toxic. According to the national mandatory standard "GB2760 Food Additive Use Standard", mogroside can be used in various foods without limit. [0003] In the current production practice, the industrial production and utilization of Luo Han Guo mainly focuses on the extraction and separation of sweet glycosides in the fruit of L...

Claims

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Application Information

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IPC IPC(8): C07J71/00C07J9/00C12P33/20C12P33/06
CPCC07J71/0005C07J9/005C12P33/20C12P33/06
Inventor 贺进军李伟黄华学黄俊宋谷良江小龙
Owner HUNAN HUACHENG BIOTECH
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