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Method for open culture and production of microalgae grease

An open culture and microalgae oil technology is applied in the field of open culture and production of microalgae oil for preventing and controlling harmful biological pollution, which can solve problems such as contamination by miscellaneous bacteria, and achieve the advantages of accumulation, easy availability of raw materials and simple operation. Effect

Active Publication Date: 2021-07-16
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of the invention can solve the problem of bacterial contamination in the open culture of microalgae, and improves the yield of oil harvest

Method used

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  • Method for open culture and production of microalgae grease
  • Method for open culture and production of microalgae grease

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) In a 20L open photobioreactor, add Scenedesmus HY-D3 seed liquid and microalgae medium, the amount of seed liquid added is 800mL, the initial pH value of microalgae medium is adjusted to 8, and the amount added is 8L , for alternating light and dark culture, the light-dark cycle is 24h, the light-dark time ratio is 14:10, the light intensity is 5000Lux, the culture temperature is 20°C, and the light reaction is passed into CO 2 CO in gas 2 The volume content is 10%, and the dark reaction is fed with SO 2 SO in gas 2 The volume content is 0.05%, which comes from the S-zorb regeneration tail gas.

[0033] (2) After 3 days of culture, reduce the light intensity to 3000 Lux on the basis of the original light, enter the stable growth period after 5 days of culture, end the culture, and detect that the number of miscellaneous bacteria is less than 2×10 3 (pieces / mL). The microalgae cells were harvested by centrifugation, and the dry cell weight and oil content were de...

Embodiment 2

[0035] With embodiment 1, difference is: the add-on of seed liquid is 800mL, and the add-on of microalgae culture medium is 4L, CO 2 Volume content is 10%, SO 2 The volume content is 0.06%. After the end of the culture, the number of miscellaneous bacteria is lower than 1.1×10 3 (unit / mL), the dry cell weight can reach 13.2g / L, and the oil content is 47.3% of the dry cell weight.

Embodiment 3

[0037] With embodiment 1, difference is: the addition amount of seed liquid is 400mL, the addition amount of microalgae culture medium is 8L, inoculum size is 1:20, CO 2 The volume content is 25%, SO 2 The volume content is 0.04%. After the end of the culture, the number of miscellaneous bacteria is lower than 2.5×10 3 (unit / mL), the dry cell weight can reach 12.2g / L, and the oil content is 45.6% of the dry cell weight.

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Abstract

The invention relates to a method for open culture and production of microalgae grease. The method comprises the following steps: adding a scenedesmus HY-D3 seed solution and a microalgae culture medium into an open photobioreactor, carrying out light-dark alternate culture, introducing CO2-containing gas during light reaction, introducing SO2-containing gas during dark reaction, carrying out alternate culture for a period of time, reducing the illumination intensity, not introducing SO2-containing gas any more, culturing to a stable phase, and harvesting microalgae cells. According to the method, the problem of infectious microbe pollution in open culture of microalgae can be solved, and the yield of grease is increased.

Description

technical field [0001] The invention belongs to the technical field of biomass energy, and in particular relates to an open cultivation method for preventing and controlling the pollution of harmful organisms and a method for producing microalgae oil. [0002] technical background [0003] Microalgae are rich in protein, polysaccharides, unsaturated fatty acids and other nutrients (such as spirulina), which can be used in food, medicine and energy; they can accumulate fatty acids in large quantities, and the fatty acid content of some microalgae can account for 30%-60% of dry weight. Using the cultivation of microalgae to accumulate oil resources has become the most popular research field in the development of renewable resources using solar energy. It not only has strong market potential, but also has extraordinary social value. [0004] At present, there are two types of microalgae cultivation methods: closed and open. Closed type refers to closed reactors with different s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCC12N1/12C12P7/6463
Inventor 师文静樊亚超张霖廖莎李晓姝
Owner CHINA PETROLEUM & CHEM CORP
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