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Open-type culture method of microalgae

A cultivation method and technology of microalgae, applied in the field of open cultivation of microalgae to prevent and control the pollution of harmful organisms, can solve problems such as the pollution of harmful organisms, achieve easy availability of raw materials, increase the growth rate, and solve the pollution of miscellaneous bacteria, diseases and insect pests The effect of preventing problems

Active Publication Date: 2014-05-07
CHINA PETROLEUM & CHEM CORP +1
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AI Technical Summary

Problems solved by technology

This method can solve the problem of harmful organisms pollution in the open culture of microalgae, effectively inhibit miscellaneous bacteria and prevent and control the pollution of diseases and insect pests. At the same time, it has the advantages of low price, safety and reliability, and will not produce resistance to subsequent subcultures.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 The inhibitory effect of the extract on miscellaneous bacteria during the cultivation process and the control effect of diseases and insect pests.

[0024] Inoculate the algae liquid of Chlorella in the logarithmic growth phase into a 20L open raceway pool reactor, the medium is SE medium, and different extracts are added along with the medium at the beginning of the culture. The initial density of Chlorella after inoculation was 1 × 10 6 Individual / mL, culture experiments were carried out with different contents of plant extracts. The culture temperature is 25°C, the culture light is 3000lux, the light-dark cycle is 24h, and the light-dark time ratio is 14:10 (hours). After 6 days of culture, the number of chlorella and the number of bacteria, diseases and insect pests are counted under the microscope by blood counting method. The numbers were observed and counted, and the results are shown in Table 1.

[0025] Table 1 Monitoring results of Chlorella contam...

Embodiment 2

[0028] Example 2 Effects of different concentrations of plant extracts on the biomass and oil content of microalgae.

[0029] Scenedesmus obliques in the logarithmic growth phase was inoculated in a 10L light reactor, using BG11 medium, and the initial density of the culture medium after inoculation was 1.21×10 6 Individual / mL, add different extracts to the culture medium for cultivation. After 10 days of cultivation, the growth of Scenedesmus obliquus is in a stable phase. The algae liquid is collected by centrifugation and dried to obtain dry algal powder, and the final biological algae are obtained by weighing. The oil content was determined by Soxhlet extraction method. The obtained results are shown in Table 2.

[0030] Table 2 The final biomass and oil content of Scenedesmus with different extracts

[0031] Extract type Extract content (mg / L) Final biomass (g / L) Fat content (mass fraction%) none 0 1.986 29.12 Neem 5 2.001 30.34 Toose...

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PUM

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Abstract

The invention relates to an open-type culture method of microalgae. The method is as below: a conventional culture method and conditions are used for microalgae culture, and a plant extract is directly added in the microalgae culture medium at the beginning of microalgae cultivation, wherein the plant extract is one or more selected from a neem extract, a Melia toosendan extract and a angled bittersweet extract, and the addition is 5-80 mg / L. The method provided by the invention can solve the problem of enemy creature pollution in open-type culture of microalgae and effectively control pollution of infectious microbes and pests in open-type cultivation, and also has the advantages of low cost, safety, reliability, and no resistance to subsequent subculture.

Description

technical field [0001] The invention belongs to the technical field of microalgae biology, and in particular relates to a method for openly cultivating microalgae for preventing and controlling the pollution of harmful organisms. technical background [0002] Microalgae are rich in protein, polysaccharides, unsaturated fatty acids and other nutrients (such as spirulina), which can be used in food, medicine and energy; they can accumulate fatty acids in large quantities, and the fatty acid content of some microalgae can account for 30%-60% of dry weight. Using the cultivation of microalgae to accumulate oil resources has become the most popular research field in the development of renewable resources using solar energy. It not only has strong market potential, but also has extraordinary social value. [0003] At present, there are two types of microalgae cultivation methods: closed and open. Closed type refers to closed reactors with different structures, such as airlift typ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 师文静张霖廖莎李晓姝王领民高大成吴丹王崇辉
Owner CHINA PETROLEUM & CHEM CORP
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