Open-type culture method of microalgae

A cultivation method and technology of microalgae, applied in the field of open cultivation of microalgae to prevent and control the pollution of harmful organisms, can solve problems such as the pollution of harmful organisms, achieve easy availability of raw materials, increase the growth rate, and solve the pollution of miscellaneous bacteria, diseases and insect pests The effect of preventing problems

Active Publication Date: 2014-05-07
CHINA PETROLEUM & CHEM CORP +1
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AI Technical Summary

Problems solved by technology

This method can solve the problem of harmful organisms pollution in the open culture of microalgae, effectively inhibit miscellaneous bacteria and prevent and control the po

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1 The inhibitory effect of the extract on the miscellaneous bacteria and the control effect of pests and diseases during the culture process.

[0024] The chlorella algae in the logarithmic growth phase was inoculated into a 20L open racetrack tank reactor, the medium was SE medium, and different extracts were added to the medium at the beginning of the culture. The initial density of Chlorella after inoculation is 1×10 6 Pieces / mL, using different content of plant extracts for culture experiments. The culture temperature is 25℃, the culture light is 3000lux, the light-dark cycle is 24h, and the light-dark time ratio is 14:10 (hours). After 6 days of culture, the number of chlorella, as well as the number of bacteria, diseases and insect pests, is measured under the microscope by hemocytometer. The number is observed and counted, and the results are shown in Table 1.

[0025] Table 1 Monitoring results of chlorella pollution by adding different plant extracts

[0026...

Example Embodiment

[0028] Example 2 The effect of plant extracts of different concentrations on microalgae biomass and oil content.

[0029] The Scenedesmus obliquus in the logarithmic growth phase was inoculated into a 10L light reactor, using BG11 medium, and the initial density of the culture solution after inoculation was 1.21×10 6 Pcs / mL, add different extracts to the culture solution for cultivation. After 10 days of culture, Scenedesmus obliquus grows in a stable period. The algae liquid is collected by centrifugation and dried to obtain dry algae powder, and the final organisms are obtained by weighing. And use Soxhlet extraction method to determine the fat content. The results obtained are shown in Table 2.

[0030] Table 2 Final biomass and oil content of Scenedesmus with different extracts

[0031] Type of extract Extract content (mg / L) Final biomass (g / L) Oil content (mass fraction%) no01.98629.12 Neem52.00130.34 Chuan Neem52.14530.23 Bitter vine52.13630.15 Neem201.98530.58 Chua...

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Abstract

The invention relates to an open-type culture method of microalgae. The method is as below: a conventional culture method and conditions are used for microalgae culture, and a plant extract is directly added in the microalgae culture medium at the beginning of microalgae cultivation, wherein the plant extract is one or more selected from a neem extract, a Melia toosendan extract and a angled bittersweet extract, and the addition is 5-80 mg / L. The method provided by the invention can solve the problem of enemy creature pollution in open-type culture of microalgae and effectively control pollution of infectious microbes and pests in open-type cultivation, and also has the advantages of low cost, safety, reliability, and no resistance to subsequent subculture.

Description

technical field [0001] The invention belongs to the technical field of microalgae biology, and in particular relates to a method for openly cultivating microalgae for preventing and controlling the pollution of harmful organisms. technical background [0002] Microalgae are rich in protein, polysaccharides, unsaturated fatty acids and other nutrients (such as spirulina), which can be used in food, medicine and energy; they can accumulate fatty acids in large quantities, and the fatty acid content of some microalgae can account for 30%-60% of dry weight. Using the cultivation of microalgae to accumulate oil resources has become the most popular research field in the development of renewable resources using solar energy. It not only has strong market potential, but also has extraordinary social value. [0003] At present, there are two types of microalgae cultivation methods: closed and open. Closed type refers to closed reactors with different structures, such as airlift typ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 师文静张霖廖莎李晓姝王领民高大成吴丹王崇辉
Owner CHINA PETROLEUM & CHEM CORP
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