Efficient microbial agent for solving successive cropping obstacles
A technology of high-efficiency microorganisms and continuous cropping obstacles, applied in the field of high-efficiency microbial inoculants, can solve the problems of falling off of microbial inoculants and synergistic substances, low utilization rate of composite inoculum compositions, and insignificant use effects, and achieves elimination of continuous cropping obstacles, The effect of improving crop stress resistance and improving stress resistance
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Embodiment 1
[0053] A high-efficiency microbial agent for solving continuous cropping obstacles is composed of fermentation broth and calcium carbonate.
[0054] The preparation method of the high-efficiency microbial agent for solving continuous cropping obstacles is as follows: the fermentation liquid and calcium carbonate are mixed according to the mass ratio of 1:2 and stirred at 800 rpm for 30 minutes, and then dried at 42°C for 5 hours to obtain the described solution for continuous cropping obstacles efficient microbial agent.
[0055] The preparation method of described fermented bacterial liquid comprises the following steps:
[0056] (1) Use the inoculation loop to pick the bacteria and inoculate them in the liquid seed medium, and cultivate them at a constant temperature at 26°C and 160rpm until the bacterial concentration reaches 10 8 cfu / mL to obtain seed liquid; the liquid seed medium is as follows: 24 parts by weight of glucose, 18 parts by weight of ammonium sulfate, 12 pa...
Embodiment 2
[0060] A high-efficiency microbial agent for solving continuous cropping obstacles is composed of fermentation broth and calcium carbonate microspheres.
[0061] The preparation method of the high-efficiency microbial agent for solving continuous cropping obstacles is as follows: the fermentation liquid and calcium carbonate microspheres are mixed according to the mass ratio of 1:2 and stirred at 800rpm for 30min, and then dried at 42°C for 5h to obtain the solution High-efficiency microbial agent for continuous cropping obstacles.
[0062] The preparation method of described fermented bacterial liquid comprises the following steps:
[0063] (1) Use the inoculation loop to pick the bacteria and inoculate them in the liquid seed medium, and cultivate them at a constant temperature at 26°C and 160rpm until the bacterial concentration reaches 10 8 cfu / mL to obtain seed liquid; the liquid seed medium is as follows: 24 parts by weight of glucose, 18 parts by weight of ammonium sul...
Embodiment 3
[0068] A high-efficiency microbial agent for solving continuous cropping obstacles consists of fermentation broth and functionalized calcium carbonate microspheres.
[0069] The preparation method of the high-efficiency microbial agent for solving continuous cropping obstacles is as follows: the fermentation broth and functionalized calcium carbonate microspheres are mixed according to the mass ratio of 1:2 and stirred at 800rpm for 30min, and then dried at 42°C for 5h to obtain the obtained A high-efficiency microbial agent to solve continuous cropping obstacles is described.
[0070] The preparation method of described fermented bacterial liquid comprises the following steps:
[0071] (1) Use the inoculation loop to pick the bacteria and inoculate them in the liquid seed medium, and cultivate them at a constant temperature at 26°C and 160rpm until the bacterial concentration reaches 10 8 cfu / mL to obtain seed liquid; the liquid seed medium is as follows: 24 parts by weight ...
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