Cell transfection system and method

Abstract

The invention discloses a cell transfection system and method. The system comprises: a bottom cover plate integrated with a large-area-array nanoneedle array structure, and a top cover plate composed of PDMS with parallel flow channels, wherein the bottom cover plate and the top cover plate form a microfluidic system in a bonding manner, and a transfection reagent and cells to be transfected pass through a large-area-array nanoneedle array region through a parallel flow channel at a specific flux; the power supply module is connected with the vibration module and the control module and is used for supplying power to the cell transfection system; the control module is connected with the vibration module and the micro-fluidic system and is used for sending a control instruction and controlling the fluid speed of the transfection reagent and the cells to be transfected; the vibration module is connected with the micro-fluidic system and is used for vibrating according to the control instruction and driving the micro-fluidic system to vibrate, so that the to-be-transfected cells are in contact with the large-area-array nanoneedle array, and the transfection reagent enters the to-be-transfected cells. The system can realize high-throughput transfection, has higher transfection efficiency and stability, and is low in cost and easy to control.

Description

Technical field [0001] The present invention relates to the field of cell transfection, and more particularly to a cell transfection system and method. Background technique [0002] Due to its own advantages such as its specificity, efficient, multi-objective editing, the CRISPR has become the most important genetic editing method, and in recent years, it has been widely used in various biological research and clinical applications and is solving cancer immunotherapy. Plays an important role in it. Safe and efficient cell transfection means is especially important in the editing of genome. Conventional intracellular transfection techniques include inducing cell membrane perforations and endocytosis using liposome or polymer nanoparticles, and the polypeptide mediated cell membrane penetration completion. These methods are somewhat limited to the structure of the cell type and the target molecule, and the transfection is less efficient. Virus transfection (such as reverse transcri...

AI Technical Summary

Problems solved by technology

Viral transfection (such as retroviral transfection, etc.) requires specific binding, which cannot achieve the versatility of cell transfection, and there are problems such as residual chemical components, which pose a safety hazard

Electroporation technology can achieve high-efficiency target transfection by breaking the membrane with current, but it has irreversible damage to cells, which limits its application

Microinjection transfection technology is inefficient and cannot achieve high-throughput transfection of bulk cells

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