Evaluation method using human stem cells, drug evaluation method, and detection kit

Abstract

The present invention relates to an evaluation method using human stem cells, a drug evaluation method, and a detection kit using human stem cells. The evaluation method using the human stem cells is characterized by comprising the following steps: (1) providing in-vitro stem cells; (2) adding a substance to be detected, and enabling the substance to act on the in-vitro stem cells in the step (1); (3) detecting the condition of the in-vitro stem cells after the step (2) is completed; and (4) evaluating the influence of the substance to be detected on the stem cells according to the condition of the in-vitro stem cells detected in the step (3). According to the evaluation method using the human body stem cells, the drug evaluation method using the human body stem cells and the detection kit using the human body stem cells, a quantitative, rapid and low-cost in-vitro detection platform and evaluation system can be established, animal experiment detection can be replaced, and a detection basis of a stem cell level is provided for in-vitro detection of a human body.

Description

technical field [0001] The invention relates to an evaluation method using human stem cells, a drug evaluation method using human stem cells and a detection kit using human stem cells. Background technique [0002] From R&D to marketing of new drugs, it needs to go through four time-consuming and costly processes: R&D, screening-preclinical research, clinical stage, and new drug application and approval for marketing. Generally, it takes more than 10 years and costs hundreds of millions or even billions of dollars. The main factors affecting the success of new drugs are the determination of drug toxicity and effectiveness in the preclinical experiment stage. Early accurate and effective toxicity determination is the key to ensure the success of the drug. [0003] At present, the evaluation of pharmacological and toxicological effects of drugs in the preclinical experimental stage is mainly carried out at the animal level. The current animal assay systems used to analyze th...

AI Technical Summary

Problems solved by technology

[0003] At present, the evaluation of pharmacological and toxicological effects of drugs in the preclinical experimental stage is mainly carried out at the animal level. The current animal assay systems used to analyze the toxic effects of potential drug molecules have considerable limitations. Not only do they need to use a large number of animals, but they are also time-consuming. Huge, generally takes 2-4 years, and the toxicity test results derived from animals cannot accurately represent the human body's response, and it is difficult to standardize

Part of the evaluation of pharmacological and toxicological effects is also carried out at the level of human cells, but the measurement indicators are limited to individual organs or tissues of the human body, lacking systemic and comprehensive

Images