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Preparation process of nitrocellulose membrane

A technology of nitrocellulose membrane and preparation process, which is applied in the field of nitrocellulose membrane preparation, can solve problems affecting the quality of nitrocellulose membrane, achieve the effects of ensuring quality and production continuity, reducing impurities, and improving quality

Active Publication Date: 2021-07-23
SHENZHEN CONNECTICUT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, when producing nitrocellulose membranes, there will be a large number of air bubbles and impurities on the surface of the nitrocellulose membranes, which will easily cause abnormal phenomena such as the solution being inclined at 45 degrees or surrounding wetting when the nitrocellulose membranes are running, which will affect the nitrocellulose membrane. film quality

Method used

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  • Preparation process of nitrocellulose membrane
  • Preparation process of nitrocellulose membrane
  • Preparation process of nitrocellulose membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] refer to figure 1 and figure 2 , the preparation process of NC film is as follows:

[0052] (1) Preparation of NC membrane paddle: Measure 100L of acetone and pour it into the liquid mixing tank, add 25kg of nitrocellulose, start the stirrer at 65r / min and stir for 3 hours, then add 45L of ethanol, 40L of n-butanol and 5L of glycerin to continue stirring. Stir for 3 days. Observe the solids in the stirred sample to complete the dissolution of the NC membrane paddle;

[0053] (2) Filtration NC membrane membrane paddle: use oil-proof polytetrafluoroethylene (PTFE) filter membrane paddle, and place it for 3 days;

[0054] (3) NC film paddle coating molding: PE film is closely attached to the stainless steel, the NC film paddle solution is injected on the PE film, and the NC film paddle solution runs with the PE film on the stainless steel belt at a constant speed;

[0055] The membrane paddle solution of the NC membrane passes through the constant temperature box at 2...

Embodiment 2

[0061] The difference between Example 2 and Example 1 is that in step (1), measure 110L of acetone and pour it into the liquid mixing tank, add 25kg of nitrocellulose, turn on the agitator at 65r / min and stir for 3 hours, then add 40L of ethanol, 50L of n-butanol and 6L of glycerol continued to stir for 3 days. Observe the situation of the solids in the stirred sample to complete the dissolution of the membrane paddle;

[0062] In step (3), the humidity of the air is 65%;

[0063] In step (4), the water temperature of the NC membrane hot water treatment is 60°C.

Embodiment 3

[0065] The difference between Example 3 and Example 1 is that in step (1), 90L of acetone is measured and poured into the liquid mixing tank, 27kg of nitrocellulose is added, the stirrer is turned on at 65r / min and stirred for 3 hours, and then 50L of ethanol, 40L of n-butanol and 6L of glycerol continued to stir for 3 days. Observe the situation of the solids in the stirred sample to complete the dissolution of the membrane paddle;

[0066] In step (4), the water temperature of the NC membrane hot water treatment is 66°C.

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Abstract

The invention relates to the technical field of nitrocellulose membrane preparation, in particular to a preparation process of a nitrocellulose membrane, which comprises the following steps: (1) preparing nitrocellulose membrane slurry; (2) filtering the membrane slurry obtained in the step (1); (3) coating and forming the membrane slurry in the step (2), and preparing a nitrocellulose membrane, namely an NC membrane, by adopting a stepped heating mode; (4) treating the surface of a NC membrane; wherein the surface treatment of the NC membrane comprises the step of treating the surface of the NC membrane through hot water and PBS in sequence; and (5) drying the NC membrane. The preparation process can be applied to production of the NC membrane, bubbles and impurities generated by the NC membrane can be effectively reduced, and the production quality of the NC membrane is improved.

Description

technical field [0001] This application relates to the technical field of nitrocellulose membrane preparation, more specifically, it relates to a nitrocellulose membrane preparation process. Background technique [0002] Nitrocellulose (NC) membrane is the main component of in vitro diagnostic reagents (immunochromatography). It is the carrier of the quality control line C line and the detection line T line. It is also the area where antibodies and antigens react. Affect the performance indicators of in vitro diagnostic reagents. Nitrocellulose membranes have been widely used in the fields of biochemistry and molecular biology, especially in molecular hybridization, western blotting, cell culture and medical diagnosis. [0003] Nitrocellulose membrane is one of the main materials of the well-known new coronavirus (antibody) detection reagent. Due to the global spread of the epidemic, the antibody reagent is in great demand, and the demand for nitrocellulose membranes has n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J5/18C08J7/12C08L1/18
CPCC08J5/18C08J7/12C08J2301/18
Inventor 宋亚琳陈立梅
Owner SHENZHEN CONNECTICUT BIOTECH
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