Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nano single hole based on capillary tip, preparation method and application thereof

A capillary, cutting-edge technology, applied in the field of nanopores, can solve the problems of difficult ion current blockage events, judging molecular structure information, etc., and achieve the effects of high application value, simple raw materials, and mild conditions.

Active Publication Date: 2021-07-23
NANJING UNIV
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nanopore single-molecule electrochemical detection technology has developed rapidly in the detection of single-molecule behavior, but it is difficult to accurately determine molecular structure information directly through the characteristic electrical signals of ion currents generated when molecules or ions pass through nanopores
Especially in the case of small molecular size, ionic current blockage events are also difficult to be captured by the instrument

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nano single hole based on capillary tip, preparation method and application thereof
  • Nano single hole based on capillary tip, preparation method and application thereof
  • Nano single hole based on capillary tip, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The method for preparing nano single hole of the present invention, comprises the steps:

[0043] (1) Preparation of capillary tip: The instrument used is the P-2000 needle pulling instrument of SUTTER Company in the United States, and the capillary is a quartz capillary (QF: 100-70-10, OD: 1.00mm, ID: 0.70mm) of SUTTER Company, the total length It is 10cm. Set parameters as heat=750, filament=3, velocity=40, delay=175, pull=190.

[0044] The diameter of the tip of the capillary prepared under the above conditions with this specification capillary is 60nm, such as Figure 4As shown in A. The capillary has a total length of 2 cm and the rear end is macroscopic.

[0045] (2) Preparation of deposition layer by chemical reduction method: inject 100mM chloroauric acid solution into the capillary, and immerse the tip of the capillary in 5mM NaBH at room temperature (25±1°C) 4 Carry out chemical reduction in pure ethanol solution, react for 1 to 2 minutes, and deposit a ta...

Embodiment 2

[0052] (1) By adjusting the parameters, a quartz capillary with a length of 10 cm and a tip diameter of 20 nm was prepared.

[0053] (2) Preparation of deposition layer by chemical reduction method: inject 200mM chloroauric acid solution into the capillary, at room temperature (25±1°C), immerse the tip of the capillary in 100mM ascorbic acid solution for chemical reduction, react for 10min, in the capillary tip A conical gold deposit is deposited.

[0054] (3) Preparation of nano single hole: inject 200mM silver nitrate solution into the capillary, then immerse the tip of the capillary in the 5mM silver nitrate solution, insert a platinum wire electrode inside and outside the tube respectively, as working electrode and counter electrode, said The working electrode and the counter electrode were directly applied with a voltage of -1 V by the current-time method of constant voltage. The gold deposition layer formed by chemical reduction is used as a bipolar electrode, and the e...

Embodiment 3

[0057] (1) By adjusting the parameters, a glass capillary with a length of 15 cm and a tip diameter of 200 nm was prepared.

[0058] (2) Electron beam evaporation technology is used to deposit a silver layer on the inner wall of the tip of the capillary.

[0059] (3) Preparation of nanometer single hole: inject 100mM chloroauric acid solution into the capillary, then immerse the tip of the capillary in 20mM chloroauric acid solution, insert a platinum wire electrode inside and outside the tube respectively as the working electrode and the counter electrode, The working electrode and the counter electrode are directly applied with a voltage of 0 to -5V by linear sweep voltammetry with a sweep rate of 200mV / s (the instrument used is CHI-830B, CH Instrument Ins). The silver deposition layer is used as a bipolar electrode, and the electrochemical reduction is terminated after the characteristic current drops, and a more uniform single-pore gold is finally reduced at the tip. The ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Tip diameteraaaaaaaaaa
Lengthaaaaaaaaaa
Login to View More

Abstract

The invention relates to a nano single hole based on a capillary tip, a preparation method and application thereof. The nano single hole is characterized in that the inner side wall of a capillary tip is provided with a deposition layer, the deposition layer extends out of a capillary to form a hollow conical structure, and a nano single hole is located at the tip of the conical structure. According to the invention, the nano single hole is simple in preparation method, low in cost, small in size and controllable in structure, and can be used as an electrochemical device to be combined with Raman spectroscopy; and bias voltage is applied to the two ends of a nanotube, so that the detection of a single molecule with a small size by dynamic Raman is realized, the detection of a single protein molecule based on current and Raman spectrum is realized, and a new method is provided for DNA sequencing and protein sequencing.

Description

technical field [0001] The invention belongs to the technical field of nanopores, and in particular relates to a nanometer single hole based on a capillary tip and a preparation method and application thereof. Background technique [0002] Nanopore analysis technology has an extremely important application in single-molecule detection, and is expected to be applied to DNA sequencing and protein sequencing. Nanopore single-molecule electrochemical detection technology has developed rapidly in the detection of single-molecule behavior, but it is difficult to accurately determine molecular structure information directly through the characteristic electrical signals of ion currents generated when molecules or ions pass through nanopores. Especially in the case of small molecular size, ionic current blockage events are also difficult to be captured by the instrument. Contents of the invention [0003] In view of the above problems, the present invention provides a nano single ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/65G01N27/48B82Y15/00B82Y40/00
CPCG01N21/65G01N27/48B82Y15/00B82Y40/00
Inventor 王康周鹃吉丽娜夏兴华
Owner NANJING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products