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Pharmaceutical composition comprising lactobacillus sakei wikim30 as active ingredient for prevention or treatment of cancer

A technology of Lactobacillus sarketi and active ingredients, which is applied in the direction of medical preparations containing active ingredients, Lactobacillus, drug combinations, etc., can solve the problem of no anti-cancer activity and excellent report of Lactobacillus sarketi, and achieve excellent The effect of anticancer activity

Pending Publication Date: 2021-07-23
KOREA FOOD RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Currently, Korean Patent Publication No. 10-2015-0068061 discloses the anticancer activity of Lactobacillus plantarum PNU (KCCM11352P) or Lactobacillus mesenteroides PNU (KCCM11353P), Korean Patent No. 10-1287120 discloses a pharmaceutical composition for treating cancer containing Lactobacillus plantarum DSR CK10 (preservation number: KFCC-11433P) or Lactobacillus plantarum DSR M2 (preservation number: KFCC-11432P) as an active ingredient, but there is still no information about Shaq Report on Anticancer Activity and Excellence of Lactobacillus

Method used

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  • Pharmaceutical composition comprising lactobacillus sakei wikim30 as active ingredient for prevention or treatment of cancer
  • Pharmaceutical composition comprising lactobacillus sakei wikim30 as active ingredient for prevention or treatment of cancer
  • Pharmaceutical composition comprising lactobacillus sakei wikim30 as active ingredient for prevention or treatment of cancer

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Experimental program
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Effect test

Embodiment 1

[0059] Embodiment 1. Isolation and cultivation of bacterial strains

[0060] Lactobacillus sarkeri WIKIM30 (preservation number: KCCM11878P) was obtained from the microbial gene bank of the World Kimchi Research Institute for experiments.

[0061] After the single bacterium colony of the obtained Lactobacillus sarkeri WIKIM30 was subcultured in the MRS agar (agar) medium, after cultivating at a temperature of 30° C. for 48 hours, the single bacterium colony of the culture was inoculated in the MRS liquid medium of 10 ml, Incubate at a temperature of 37° C. with shaking at a frequency of 200 rpm for 24 hours. After culturing, the culture medium was removed by centrifugation at 8000 rpm for 5 minutes, and washed three times with phosphate buffered saline (PBS, Phosphate Buffered Saline) to remove remaining medium components.

Embodiment 2

[0062] Embodiment 2. Conditions of experimental animals (mice)

[0063] The experimental animals used in the experiment were male 5-week-old BALB / c mice and C57BL / 6 mice (ORIENT BIO Company, Korea), which were kept in a specific pathogen-free ( After a stabilization period of 1 week in an animal breeding room in a SPF) environment, rearing was carried out during the experiment. Feed was provided as plain tray feed without added antibiotics, and water was provided in a ready-to-take form. Experiments All animal breeding, experimentation and euthanasia were carried out in accordance with protocols approved by the World Kimchi Research Institute Animal Experiment Ethics Committee. The observation of tumor size changes uses the method of 3.14×(length×height×width) / 6 to measure the tumor volume (mm 3 ).

Embodiment 3

[0064] Embodiment 3. Preparation of CT26 mouse colorectal cancer cell transplantation animal model

[0065] 3-1. Cell culture

[0066] CT26 mouse colorectal cancer cells (Korean Cell Line Bank, Korea) were purchased and used. Using DMEM medium (Hyclone Company, USA) containing 10% fetal bovine serum and 1% penicillin-streptomycin (Hyclone Company, USA), in 5% CO 2 1. Cultivate CT26 mouse colorectal cancer cells at 37°C.

[0067] 3-2. Preparation of cell transplantation cancer animal model

[0068] In order to prepare an animal model of CT26 cell transplantation, 6-week-old BALB / c mice (18g-21g) were used in the experiment. Get (harvest) 1×10 5 Cells of cultured mouse colorectal cancer cell CT26 were resuspended in 50 μl of phosphate buffer solution, and injected subcutaneously into the right thigh of the mouse.

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Abstract

The present invention relates to novel Lactobacillus sakei WIKIM30 (accession number KCCM11878P) isolated from Kimchi, and a composition comprising same as an active ingredient. Exhibiting excellent anticancer activity in animal models into which cancer cells have been transplanted and cancer cell lines, Lactobacillus sakei WIKIM30 according to the present invention can be advantageously used as a composition for treatment, prevention, or alleviation of cancer in humans or animals.

Description

technical field [0001] The present invention relates to a novel Lactobacillus sakei WIKIM30 (Lactobacillus sakei, deposit number: KCCM11878P) isolated from pickles and a composition containing it as an active ingredient. Background technique [0002] Cancer exhibits a high mortality rate worldwide and is the second most common cause of death after cardiovascular disease in Western European societies. In particular, the widespread intake of high-fat diets brought about by the Westernization of dietary life, the sharp increase in environmental pollutants, and the increase in alcohol consumption have led to a continuous increase in colorectal cancer, breast cancer, and prostate cancer. At the same time, lung cancer caused by the increase in the number of smokers and air pollution is also increasing. Under such circumstances, it is necessary to effectively create an anticancer substance capable of early prevention and treatment, promotion of human health, improvement of healthy...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61P35/00A23L33/135A23K10/18
CPCA23K10/18A23L33/135A61K35/747A61P35/00A23K50/10A23K50/30A23K50/40A23K50/75A23K50/50A23K50/80A23V2002/00Y02A40/818A61K2035/115A23V2400/179A23V2200/308A23V2200/3204A61K9/0019
Inventor 崔鹤钟尹美善
Owner KOREA FOOD RES INST
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