Terpenoid compound, its preparation method and its use and antibacterial agent
A terpenoid and compound technology, applied in the field of natural compounds, can solve the problems of less research on the physiological and biochemical effects of monomers, less research on terpenoid monomer substances, etc., and achieve excellent bacteriostatic effect, rich species, and excellent antibacterial effect. Effect
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preparation example Construction
[0033] The embodiment of the present invention provides a preparation method of the above-mentioned terpenoid compound, comprising:
[0034] S1, prepare the extract;
[0035] Extraction of Rhizoma Rhizoma Radix, specifically, using supercritical extraction to prepare Rhizoma Rhizoma officinalis extract, wherein, the operation of supercritical extraction includes: weighing an appropriate amount of Rhizoma Rhizoma Radix, pulverizing, placing in a supercritical extraction kettle, opening the extraction kettle and the separation kettle and adding After reaching the set temperature, turn on the compression pump; the pressure of the extraction kettle is kept at 12-15.0 MPa (for example, any value between 12-15 MPa, such as 12 MPa, 13 MPa, 14 MPa, and 15 MPa), and the temperature is 45 -50°C (for example, any value between 45-50°C such as 45°C, 47°C and 50°C), at 20-30 kg / h (for example, 20 kg / h, 25 kg / h and 30 kg / h Any value between 20-30kg / h) C0 2 Flow rate circulation extraction...
Embodiment 1
[0072] The present embodiment provides 3 terpenoids, and their structural formulas are as follows:
[0073] Formula (1) (denoted as compound 1), Formula (2) (denoted as compound 2) and Formula (3) (denoted as compound 3).
[0074] The present embodiment also provides the preparation methods of the above-mentioned 3 terpenoids, including:
[0075] Use supercritical extraction to extract Radix Radix et Rhizoma. The conditions of supercritical extraction are: weigh 10.0 kg of Radix Radix et Rhizoma, pulverize, place it in a 50L supercritical extraction kettle, turn on the heating device of the extraction kettle and the separation kettle, and wait until the set temperature is reached. Turn on the compression pump; the pressure of the extraction kettle is kept at 15.0 MPa, the temperature is 45 °C, and the CO2 of 30 kg / h is maintained. 2 Flow rate circulation extraction; separation kettle I pressure and temperature were 10.0 MPa and 40 ℃; separation kettle II pressure and te...
experiment example
[0093] Preparation of sample drug sensitive paper: filter paper is used to make circular paper with a diameter of 0.6 cm, which is sterilized and dried. The test drug compounds 1~3 were dropped on the paper, divided into three doses: 10 μ L / piece, 5 μ L / piece and 2.5 μ L / piece.
[0094] Test operation: The purified colonies and standard strains were inoculated into 5 mL of LB broth medium, and incubated at 37°C with shaking for 8-12 hours. Adjust the bacterial solution to a McFarland concentration of 0.5, and evenly coat a 90cm MH agar plate with a glass rod, then paste a drug sensitive paper on the plate, make a mark, and cultivate at a constant temperature of 37 °C. The bacteria were Staphylococcus, Escherichia coli, Salmonella and Streptococcus.
[0095] Drug susceptibility test identification results: four pathogenic bacteria, Salmonella, Escherichia coli, Staphylococcus and Streptococcus are all sensitive to several classes of antibiotics, especially the terpenoids...
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