The control method of pcr continuous reaction

Abstract

The present invention relates to a method for controlling continuous PCR reaction. The method for controlling continuous PCR reaction comprises: injecting a sample into a pipeline layer through a sample hole, injecting a lysate into the pipeline layer through a first reagent tube to form a product; The first piston connected to the first reagent tube is pulled out, and the product is pushed into the purification chamber along the pipeline; the ultrasonic unit is set under the purification chamber, and the ultrasonic unit breaks up the magnetic beads in the purification chamber. Make the nucleic acid substances in the product adsorb on the surface of the magnetic beads; start the magnetic suction unit, push the magnet in the magnetic suction unit to the bottom of the purification chamber to attract the magnetic beads; push the cleaning solution in the third reagent tube into the purification chamber , wash at least once; elute the nucleic acid material to separate it from the magnetic beads; push the nucleic acid into the amplification chamber in the pipeline layer; realize the amplification reaction. The invention realizes the control of the continuous reaction of PCR, has a higher degree of pipeline integration in the reaction process, is simple to operate, and is easy to realize.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a method for controlling continuous PCR reactions. Background technique [0002] The extraction, purification, and amplification of nucleic acid are routine operations in nucleic acid detection tests, and are also critical steps in the process of gene analysis. In the process of gene analysis, it is often necessary to separate specific nucleic acid fragments from mixed samples for subsequent PCR amplification. Therefore, the purification and recovery of nucleic acid directly affects the progress and results of the entire gene analysis process. [0003] The Chinese patent discloses a nucleic acid automatic detection device, which includes a fixed base and a main support plate vertically fixed on the fixed base, an automatic sample reaction mechanism and its fixed frame, a nucleic acid detection optical path structure and its supporting frame, and a controller; The automatic me...

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Problems solved by technology

[0004] In the above technical solutions, on the one hand, the detection device has high environmental requirements and low test accuracy. At the same time, the internal pipeline of the chip is not integrated, and it is difficult to mix and test the reaction solution as expected, and the extraction, purification and amplification of nucleic acid usually require Use different instruments to complete, and the operation is cumbersome

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