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Peptide vaccine based on activated fibroblast FAP and preparation method of the peptide vaccine

A technology of fibrogenesis and peptides, applied in the field of peptide vaccines and its preparation, can solve the problems of reduced ventricular compliance, deterioration of cardiac function, lack of diastolic and systolic functions, etc., and achieve simple structure, delay the course of the disease, and objective effects

Active Publication Date: 2021-07-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These fibrous tissues not only have no systolic function, but also reduce ventricular compliance, leading to deterioration of cardiac function
Chronic myocardial remodeling caused by pressure overload is also characterized by excessive activation and proliferation of fibroblasts, which is a compensatory mechanism of the heart, but in the decompensated period, the myocardium is replaced by a large number of fibroblasts and extracellular matrix, Consequences leading to reduction in cardiac function up to heart failure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The preparation method of the peptide vaccine based on activated fibroblast FAP is as follows:

[0039] The first step: first prepare liposomal nanoparticles by thin film method, in the process of rotary evaporation, cholesterol-PEG-linked mannose is added to the mother liquor of the above-mentioned lipid nanoparticles with a mass ratio of 1:1000 to obtain Mannose-functionalized lipid films as nanoshells;

[0040] The second step: the immune adjuvant and the FAP antigen are added into the rotary steamer bottle equipped with the above-mentioned lipid film for hydration, and obtained through ultrasonic vibration treatment.

Embodiment 2

[0042] The peptide vaccine prepared in Example 1 was intravenously injected into healthy mice and mice with symptoms of heart failure. The injection amount was 1 mg / mouse according to the amount of lipid nanoshells. After a period of observation and control, It was found that after injection of the peptide vaccine, the immune effect of mice against heart failure was significant.

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PUM

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Abstract

The invention discloses a peptide vaccine based on activated fibroblast FAP and a preparation method of the peptide vaccine, and belongs to the field of biological medicine. The peptide vaccine comprises an FAP antigen with an amino acid sequence as shown in SEQ ID NO: 1 and a nano shell capable of specifically targeting at least one antigen presenting cell of an immune system, and the FAP antigen is wrapped in the nano shell. The peptide vaccine is the first practice of cardiovascular disease immunotherapy, and compared with the defects that a traditional drug therapy can only relieve symptoms and delay the disease course and has no effect on the pathogenesis of heart failure, the peptide vaccine can terminate and even reverse the pathological process of heart failure, and a new way is provided for prevention and treatment of the diseases.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and particularly relates to a peptide vaccine based on activated fibroblast FAP and a preparation method thereof. Background technique [0002] Heart failure is a major death killer of cardiovascular disease, and it is the final outcome of various cardiovascular diseases, including ischemic heart disease, dilated heart disease, and hypertrophic heart disease. Once heart failure occurs, existing treatment methods cannot stop or reverse the progression of the disease. Although drug therapy can relieve symptoms and improve prognosis to a certain extent, the pathology of heart failure continues until the heart function is decompensated or lost. Patients with end-stage heart failure have only options for mechanical assist devices or heart transplantation, but the high cost makes these treatments difficult for most patients. Therefore, it is urgent to explore the pathogenesis of heart failure and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K39/39A61K9/127A61K47/28A61K47/26A61P9/04
CPCA61K39/00A61K39/39A61K9/1272A61K9/1277A61K47/28A61K47/26A61P9/04A61K2039/55561A61K2039/54A61K2300/00
Inventor 张宁黄浙勇葛均波
Owner ZHEJIANG UNIV