Micro-fluidic chip and device capable of being used for stably capturing CTC at ultrahigh flow speed
A microfluidic chip, chip technology, applied in enzymology/microbiology devices, laboratory containers, biochemical cleaning devices, etc., can solve the problem of the decline of target particle capture efficiency, and achieve excellent capture efficiency and capture purity. , the effect of reducing the area
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Embodiment 1
[0077] This embodiment provides a three-dimensional microfluidic chip for detecting CTC, such as figure 2 As shown, it is a double-layer chip, including: a converging and dividing layer chip 1 and a trapping layer chip 2 .
[0078] The converging and splitting layer chip 1 is provided with a converging and splitting module. The converging and splitting module includes an inlet, a filter unit 11 , six converging and splitting units 12 connected in series, and an outlet 13 connected in sequence by a main channel.
[0079] Wherein, the filter unit 11 includes a square filter structure 111 with a height of 60 microns and an S-shaped filter structure 112 with a height of 30 microns to prevent chip clogging.
[0080] The converging and splitting unit 12 includes a collecting port, a narrowing part of the main channel, five converging structures 121 and two splitting channels 122 arranged on both sides, the height of which is 60 microns. According to the calculation of flow resista...
Embodiment 2
[0088] This embodiment provides a method for manufacturing the three-dimensional microfluidic chip in Embodiment 1, such as image 3 As shown, it mainly includes the following steps:
[0089] Step S1: Prepare molds for the converging flow-splitting layer chip and the trapping layer chip respectively.
[0090] Step S2: coating the prepared material on the mold and performing curing treatment.
[0091] Step S3: Baking the connection points of the converging and separating layer chip and the capture layer chip in alignment.
[0092] Step S4: performing plasma treatment on the structure of the chip, bonding with the glass slide and baking.
[0093] In a specific example, such as Figure 4 As shown, the steps of making the structure of the three-dimensional microfluidic chip are as follows:
[0094] (1) Preparation of the mold. Draw with L-Edit and print it on plastic film or optical mask as exposure mask for subsequent experiments. In total, one silicon wafer mold is require...
Embodiment 3
[0098] This embodiment provides a method for using the three-dimensional microfluidic chip in Embodiment 1, such as Figure 5 As shown, the method mainly includes the following steps:
[0099] Step S1: Wet and pretreat the chip by injecting 1×PBS and 0.1% BSA, and let stand for 2 hours.
[0100] Step S2: Pass the detection sample into the chip for CTC capture, and the effluent liquid is collected with an orifice plate.
[0101] Step S3: Microphotographing and counting the triangular capture area of the chip and the orifice plate.
[0102] It should be noted that when performing blood sample detection, the chip needs to be wetted with heparin in the pretreatment stage, and red blood cell lysate can be passed through before microscopic counting to facilitate imaging.
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Abstract
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Application Information
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