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Method for extracting sea cucumber polypeptide from sea cucumber viscera

A technology of sea cucumber viscera and sea cucumber polypeptides, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc., which can solve the problems of comprehensive utilization of sea cucumbers with high added value, inability to fully extract sea cucumber polypeptides, and low yield of sea cucumber polypeptides and other issues, to achieve the effect of realizing high added value, realizing comprehensive utilization and improving purity

Pending Publication Date: 2021-07-30
大洲新燕(厦门)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, sea cucumber polypeptides are usually prepared by water extraction or enzymatic hydrolysis, but the produced sea cucumber polypeptides have low yield, low purity, and contain more impurities, and the sea cucumber polypeptides cannot be fully extracted, resulting in waste of resources. Failed to realize the comprehensive utilization of sea cucumber with high added value

Method used

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  • Method for extracting sea cucumber polypeptide from sea cucumber viscera
  • Method for extracting sea cucumber polypeptide from sea cucumber viscera
  • Method for extracting sea cucumber polypeptide from sea cucumber viscera

Examples

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Embodiment 1

[0027] A method for extracting sea cucumber polypeptides from sea cucumber viscera, comprising the following steps:

[0028] S1. Place 50g of sea cucumber viscera in an environment of 50°C, dry for 2 hours, then put the sea cucumber viscera into a high-speed pulverizer and pulverize them into sea cucumber viscera powder, add the sea cucumber viscera powder into water according to the material-to-liquid ratio of 1:30, and wait until After the sea cucumber viscera powder is fully swollen, it is treated in a constant temperature water bath at 50°C for 4 hours to obtain a homogenate of sea cucumber viscera;

[0029] S2. Add 3% neutral protease to the homogenate of sea cucumber viscera, adjust the temperature to 50°C, and adjust the pH value to 7.5 with sodium carbonate solution, and perform enzymatic hydrolysis for 4 hours. Inactivate in a boiling water bath for 10 minutes, after inactivation, refrigerate and centrifuge at 4°C and 10,000 r / min for 15 minutes to obtain enzymatic hy...

Embodiment 2

[0035] A method for extracting sea cucumber polypeptides from sea cucumber viscera, comprising the following steps:

[0036] S1. Place 50g of sea cucumber viscera in an environment of 50°C, dry for 2 hours, then put the sea cucumber viscera into a high-speed pulverizer and pulverize them into sea cucumber viscera powder, add the sea cucumber viscera powder into water according to the material-to-liquid ratio of 1:30, and wait until After the sea cucumber viscera powder is fully swollen, it is treated in a constant temperature water bath at 50°C for 4 hours to obtain a homogenate of sea cucumber viscera;

[0037] S2. Add 3% neutral protease to the homogenate of sea cucumber viscera, adjust the temperature to 50°C, and adjust the pH value to 7.5 with sodium carbonate solution, and perform enzymatic hydrolysis for 3 hours. Inactivate in a boiling water bath for 10 minutes, after inactivation, refrigerate and centrifuge at 4°C and 10,000 r / min for 15 minutes to obtain enzymatic hy...

Embodiment 3

[0043]A method for extracting sea cucumber polypeptides from sea cucumber viscera, comprising the following steps:

[0044] S1. Place 50g of sea cucumber viscera in an environment of 50°C, dry for 2 hours, then put the sea cucumber viscera into a high-speed pulverizer and pulverize them into sea cucumber viscera powder, add the sea cucumber viscera powder into water according to the material-to-liquid ratio of 1:30, and wait until After the sea cucumber viscera powder is fully swollen, it is treated in a constant temperature water bath at 50°C for 4 hours to obtain a homogenate of sea cucumber viscera;

[0045] S2. Add 3% neutral protease to the homogenate of sea cucumber viscera, adjust the temperature to 50°C, and adjust the pH value to 7.5 with sodium carbonate solution, and perform enzymatic hydrolysis for 4 hours. Inactivate in a boiling water bath for 10 minutes, after inactivation, refrigerate and centrifuge at 4°C and 10,000 r / min for 15 minutes to obtain enzymatic hyd...

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Abstract

The invention discloses a method for extracting sea cucumber polypeptide from sea cucumber viscera, the method comprises the following steps: drying the sea cucumber viscera, crushing into sea cucumber viscera powder, soaking and swelling the sea cucumber viscera powder, and performing water bath treatment to fully decompose raw materials and facilitate the release of contents in the sea cucumber viscera powder, then utilizing the technology of secondary enzymatic hydrolysis, overcoming the product inhibition effect, fully breaking the peptide bonds in the sea cucumber viscera, promoting conversion of proteins in the sea cucumber viscera into polypeptides, improving the extraction rate of the polypeptides in the sea cucumber viscera, and finally obtaining the sea cucumber polypeptides through impurity removal and freeze drying.

Description

technical field [0001] The invention relates to the technical field of sea cucumber processing, in particular to a method for extracting sea cucumber polypeptides from sea cucumber viscera. Background technique [0002] Sea cucumbers belong to the class Echinodermata and have high economic value. There are thousands of species of sea cucumbers in the world, but only more than 40 of them are edible, and there are about 20 species in my country. Sea cucumber, also known as "sea ginseng", is named for its tonic effect similar to ginseng, and is listed as one of the "eight treasures" of seafood together with abalone. There are relevant records in "Compendium of Materia Medica": sea cucumber, sweet and salty, warming in nature, nourishing the essence, nourishing the kidney, aphrodisiac, treating impotence, taking urine, and ginseng for the enemy. Scientific research shows that sea cucumber contains high protein, low cholesterol, low sugar, low fat, and is rich in various trace ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/16
CPCC12P21/06C07K1/16
Inventor 林嘉珣黄红岺曾荣急
Owner 大洲新燕(厦门)生物科技有限公司
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