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Portable cell imaging and intelligent analysis system based on edge calculation

A technology of intelligent analysis and edge computing, applied in the field of cell detection and analysis in medicine and biology, it can solve the problems of inability to achieve simultaneous data analysis, separation of imaging and analysis modules, and inability to meet complex operations, so as to improve generalization capabilities. , easy to move, light weight effect

Pending Publication Date: 2021-07-30
PEKING UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, although the miniaturization of the imaging module maintains a fairly high-precision resolution, the image analysis module still requires a larger computer for later analysis, and the simultaneous analysis of data cannot be achieved, which separates the imaging and analysis modules.
Although there have been portable cell counters, the computing hardware of this type of system is generally based on single-chip microcomputers and embedded systems. The computing power is very weak and cannot meet the needs of complex calculations. Usually, only specific types of cells can be counted.

Method used

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  • Portable cell imaging and intelligent analysis system based on edge calculation
  • Portable cell imaging and intelligent analysis system based on edge calculation
  • Portable cell imaging and intelligent analysis system based on edge calculation

Examples

Experimental program
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Effect test

Embodiment 1

[0047] This embodiment provides a portable cell imaging and intelligent analysis system, figure 1 It is the appearance structure diagram of the system, and its externally visible parts include an image display device 11, a housing 12, an illumination device 13, an object carrying device 14, an object moving device 15 for controlling the horizontal movement of the object carrying device 14, and a handle 16, switch 17. The image display device 11 displays real-time cell images and cell analysis results and provides users with multiple operation options. The image display device 11 can also be used to control the brightness of the lighting device 13 and the height of the object-carrying device 14 .

[0048] figure 2 for figure 1 The diagram of the internal optical components used for imaging, mainly including the lighting device 13 arranged in order from top to bottom, the collimator 21 used for collimating the light source, and the slide glass 22 equipped with the sample to b...

Embodiment 2

[0058] Using the system provided in Example 1, inject the processed different cell suspensions to be tested into slides and place them on the loading device, start the system, adjust the displacement stage and the loading moving device until the image display device displays a clear image. The state of the cell can be observed and the state information of the cell can be obtained.

[0059] Figure 4 Shown are low concentrations (1.9 × 10 6 / mL) sample condition, the recognition effect of HEK293 cells; Figure 5 Shown at high concentrations (9.8×10 6 / mL) cell recognition effect under sample conditions. Among them, live cells and dead cells are distinguished according to the color of the rectangular frame: figure 1 The number of live cells was 463, and the number of dead cells was 33; figure 2 The number of live cells was 2278, and the number of dead cells was 9. Depend on figure 1 with figure 2 The identification results can show that under different cell density con...

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Abstract

The invention provides a portable cell imaging and intelligent analysis system based on edge calculation. The system comprises a lighting device, a carrying device, a lens group, a camera arranged below the lens group and used for acquiring images, an image display device for displaying cell states in real time, an ARM-based microcomputer mainboard-carried general-purpose computer operating system, and a deep learning model edge reasoning calculation acceleration device, an intelligent software based on a deep learning instance segmentation algorithm Mask R-CNN, a power supply device and a shell. According to the analysis system provided by the invention, real-time cell imaging, identification, counting and related morphological analysis can be realized only through simple operation.

Description

technical field [0001] The invention relates to a portable cell imaging and intelligent analysis system based on edge computing, which belongs to the field of cell detection and analysis in medicine and biology. Background technique [0002] Cells are important objects of study in fields such as medicine and biology. In medical testing, diseases can be diagnosed through cell morphology and quantity; in biological experiments, the growth and proliferation status of isolated cells during culture is an important indicator in scientific research. For mixed cell samples with different types and forms, such as complete blood count, urine cell detection, etc., how to perform accurate and efficient analysis while performing high-precision imaging is a very important technical issue. At present, the common analysis and counting methods of cells include microscope observation, electrical impedance counting and image analysis. However, the microscope observation method requires the o...

Claims

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Application Information

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IPC IPC(8): G06T7/11G06K9/62G06M11/02G06T1/20G06N3/04G06N3/08G06N5/04
CPCG06T7/11G06M11/02G06T1/20G06N3/08G06N5/04G06T2207/10056G06N3/045G06F18/24
Inventor 张晨王仰真王冲苏峰唐艺岚魏梦萍
Owner PEKING UNIV
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