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Nano-carrier for chemotherapy and photothermal therapy combined therapy of tumors and preparation method and application thereof

A nano-carrier and combined therapy technology, applied in the field of biomedical materials, can solve the problem that it is not enough to cure cancer completely, and achieve the effect of simplifying the preparation

Inactive Publication Date: 2021-08-06
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemotherapy is a widely used treatment in clinic, but due to the intolerable side effects brought by chemotherapy drugs and the complexity of tumor physiology, chemotherapy alone may not be enough to completely cure cancer

Method used

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  • Nano-carrier for chemotherapy and photothermal therapy combined therapy of tumors and preparation method and application thereof
  • Nano-carrier for chemotherapy and photothermal therapy combined therapy of tumors and preparation method and application thereof
  • Nano-carrier for chemotherapy and photothermal therapy combined therapy of tumors and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Preparation of nanocarriers and nanomedicines

[0037] 1) Dissolve 0.68 g HOOC-ICG-COOH in 50 mL N,N-dimethylformamide, add 0.7 mL ethylenediamine, 0.2 g EDC, 0.12 g NHS, and react overnight at room temperature; dialysis, lyophilize, and purify to obtain H 2 N-ICG-NH 2 . Dissolve 50 mL in N,N-dimethylformamide again, add 1.7 g HOOC-ICG-COOH, 0.5 g EDC, 0.3 g NHS, react overnight at room temperature, dialysis, lyophilize, and purify to obtain PICG.

[0038] 2) 0.2 g mPEG-Mal (molecular weight: 2 KDa) and 0.1 mL 2-furan methylamine were dissolved in 20 mL acetone, heated to 50 °C, stirred overnight, and purified to obtain mPEG-DA. Then, the above mPEG-DA and 0.2 g PICG were dissolved in 20 mL N,N-dimethylformamide, and catalyzed by 0.2 g EDC and 0.12 g NHS, reacted overnight at room temperature, lyophilized by dialysis, and purified to obtain PEG-DA- PICG.

[0039] 3) Dissolve 50 mg of PEG-DA-PICG and 5 mg of paclitaxel in 2 mL of dichloromethane, and then...

Embodiment 2

[0040]Example 2: Preparation of nanocarriers and nanomedicines

[0041] 1) Dissolve 0.68 g HOOC-ICG-COOH in 50 mL N,N-dimethylformamide, add 1.0 mL ethylenediamine, 0.2 g EDC, 0.12 g NHS, and react overnight at room temperature; dialysis, lyophilize, and purify to obtain H 2 N-ICG-NH 2 . Dissolve 50 mL in N,N-dimethylformamide again, add 2.1 g HOOC-ICG-COOH, 0.5 g EDC, 0.3 g NHS, react overnight at room temperature, dialyze, freeze-dry, and purify to obtain PICG.

[0042] 2) 0.5 g mPEG-Mal (molecular weight: 5 KDa) and 0.12 mL 2-furan methylamine were dissolved in 20 mL acetone, heated to 50 °C, stirred overnight, and purified to obtain mPEG-DA. Then, the above mPEG-DA and 0.2 g PICG were dissolved in 20 mL N,N-dimethylformamide, and catalyzed by 0.2 g EDC and 0.12 g NHS, reacted overnight at room temperature, lyophilized by dialysis, and purified to obtain PEG-DA- PICG.

[0043] 3) Dissolve 50 mg of PEG-DA-PICG and 5 mg of doxorubicin in 2 mL of dichloromethane, and then...

Embodiment 3

[0044] Example 3: PEG-DA-PICG verification and photothermal cracking ability verification

[0045] 50 mg of PEG-DA-PICG prepared in Example 1 was dissolved in 10 mL of ethanol, and divided into two equal parts. One without treatment and one with 808 nm laser (0.5 W / cm 3 ) for 10 min. Then, they were dialyzed in deionized water using a dialysis bag with a molecular weight cut-off of 3.5 KDa, and then lyophilized into powder. The molecular weights of the two were measured by gel permeation chromatography (GPC). The specific results are shown in Table 1. The molecular weight of unirradiated PEG-DA-PICG is 3870, which is close to the theoretical value, which is slightly smaller than the theoretical value due to a small amount of unreacted PICG. The measured molecular weight of PEG-DA-PICG after near-infrared illumination is less than 2150, which is close to the theoretical value of PICG connected to furan group, which indicates that the heat generated by near-infrared illuminati...

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Abstract

The invention discloses a nano-carrier for chemotherapy and photothermal therapy combined therapy of tumors. The nano-carrier is formed by self-assembly of an amphiphilic copolymer; a hydrophobic end of the nano-carrier consists of oligoindocyanine green; a hydrophilic end of the nano-carrier consists of polyethylene glycol; the hydrophilic end and the hydrophobic end of the amphiphilic copolymer are bonded by a thermal response group, and the molecular weight of the nano-carrier is 1-20 K Da; and the response temperature of the thermal response group is 50-90 DEG C. The nano-carrier provided by the invention has photothermal conversion capability, and can be used for chemotherapy and photothermal therapy combined therapy without being additionally coated with a photothermal conversion material. The invention further discloses a preparation method of the nano-carrier and application of the nano-carrier to preparation of nano-drugs for chemotherapy and photothermal therapy combined therapy, and in the application process, the thermal response performance and the thermal conversion capacity of the nano-carrier are coupled, and the coated drugs can be rapidly released under near-infrared illumination.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a nanocarrier used in combination therapy of tumor chemotherapy and photothermal therapy, a preparation method of the nanocarrier, and an application of the nanocarrier in preparing a nanomedicine for combined therapy of chemotherapy and photothermal therapy. Background technique [0002] Cancer cases and deaths have increased rapidly over the past decade, and cancer is projected to become the leading cause of death worldwide in the 21st century. Therefore, it is urgent to develop highly effective anticancer strategies. Chemotherapy is widely used clinically, but due to the unbearable side effects of chemotherapy drugs and the complexity of tumor physiology, chemotherapy alone may not be enough to completely cure cancer. Comprehensive treatment is a combined treatment system based on chemotherapy and other treatment methods that reinforce each other, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/60A61K31/704A61K31/337A61K31/4745A61P35/00
CPCA61K41/0057A61K47/60A61K31/704A61K31/337A61K31/4745A61P35/00A61K2300/00
Inventor 钱程沈灿柳森叶玮潘长江杨忠美魏言春权莉
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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