AIE nanofiber probe for targeted dyeing of cell membrane and preparation method of AIE nanofiber probe

Abstract

The invention discloses an AIE nano-fiber probe for targeted dyeing of a cell membrane and a preparation method of the AIE nano-fiber probe. The method comprises the following steps that a fluorescent molecule DTPM is wrapped with an aggregation-induced emission characteristic by utilizing amphiphilic molecules DSPE-PEG2000-Mal and DSPE-PEG2000, so as to prepare a nanofiber; then maleic anhydride (-Mal) on the surface of the nanofiber and a sulfydryl (-SH) reactive group on cysteine (Cys) in a polypeptide sequence are subjected to an addition reaction, a flexible chain bioactive polypeptide chain with a targeting function is introduced to the nanofiber, and finally the AIE nanofiber with positive charges and a hydrophilic and hydrophobic structure surface is prepared. The targeting property is realized through charges and hydrophilic and hydrophobic structures of the polypeptide. The method realizes targeting of AIE nanofibers to cell membrane dyeing, has the advantages of high resolution, no elution, high dyeing speed, stable performance and the like, and is expected to be used as a novel cell membrane fluorescent probe.

Description

technical field [0001] The invention relates to a fluorescent probe capable of targeting staining cell membranes, in particular to an AIE nanofiber probe targeting staining cell membranes and a preparation method thereof. Background technique [0002] The cell membrane is the channel for the exchange of information and substances between the cell and the surrounding environment. Their shape and shape are constantly changing dynamically. They are important structures for the normal physiological activities of cells such as endocytosis, exocytosis, and signal transmission. The morphology of the cell membrane is a direct sign of the cell state, for example, cell membrane rupture or partial phagocytosis is an important evidence of cell apoptosis. In addition, as the first protective barrier of cells, the cell membrane will change its shape and structure once it is damaged by external harmful substances (such as heavy metal ions). Therefore, real-time tracking and observation of...

AI Technical Summary

Problems solved by technology

However, due to the large molecular weight and strong hydrophobic structure of AIE molecules, it is difficult to modify their hydrophilicity and functionalization, and the modification may have a negative impact on their photophysical properties.

Therefore, how to realize the modification and functionalization of AIE molecules in a simple way is a problem that many researchers are studying. At present, packaging into nanostructures is a good way to solve this problem, but there is still a problem of how to modify the nanostructures. For the problem of modification to achieve specific functions, related fluorescent probes need to be developed urgently

[0004] In recent years, many researchers have devoted themselves to the research of targeted AIE nano-fluorescent probes, but these probes often have targeting difficulties, complicated preparation methods, poor photostability, difficult to achieve long-term labeling and tracking, and have negative effects on normal cells and Tissues have issues such as toxicity that need to be addressed

For example, patent CN 110231316 A discloses an AIE-Gd@SiO 2 Nanocomposite fluorescent probes that combine AIE fluorescent materials with Gd@SiO 2 Combined with complexes, nanoprobes targeting and labeling stem cells were prepared, but there are still some problems such as cytotoxicity and long time required for staining and labeling

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