Lambda-cyhalothrin degrading bacterium and application thereof
A technology of lambda-cyhalothrin and degrading bacteria, applied in the direction of bacteria, methods using microorganisms, biological water/sewage treatment, etc., can solve the problems of increasing the risk and damage of non-target biological environments
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0016] The separation method of lambda-cyhalothrin-degrading bacteria of the present invention is to screen the strains that degrade lambda-cyhalothrin from the soil that has been sprayed with lambda-cyhalothrin for a long time through the method of enrichment and acclimatization, comprising the following steps :
[0017] (1) Weigh 5g of the soil sample sprayed with lambda-cyhalothrin for a long time, add it into a culture bottle containing 100mL of inorganic salt medium, and place the culture bottle in a constant temperature shaking incubator with a temperature of 30°C and a speed of 150r / min cultivated in. Among them, inorganic salt medium (g / L): NH 4 NO 3 1.0g, NaCl 1.0g, KH 2 PO 4 1.5g, K 2 HPO 4 0.5g, MgSO 4 0.1g, FeSO 4 0.025g, 1000mL deionized water, adjust the pH to 7.0.
[0018] (2) Step (1) After culturing the culture bottle for 5 days, take it out and let it stand, take 5 mL of the supernatant and transfer it to a culture bottle containing 100 mL of 50 mg / ...
Embodiment 2
[0024] Degradation of lambda-cyhalothrin in water by Pallidum kienei:
[0025] The high-efficiency degradation strain Ochrobactrum intermedium (Ochrobactrum intermedium) obtained by domestication was cultivated in beef extract peptone medium to prepare OD 600 =1.0 bacterial suspension. Under aseptic conditions, the bacterial suspension of strain LB1 was connected to the sterilized inorganic salt medium at an inoculum size of 10%, and then the efficient cyhalothrin standard solution was added to make the high-efficiency chlorine in the final inorganic salt culture medium The concentration of cyfluthrin was 100mg / L, and the inorganic salt culture medium without bacteria was used as the blank experiment control of abiotic degradation. Three parallel samples were set up, and all operations were carried out under sterile conditions. The culture solution was placed in a constant temperature double-layer shaking incubator at 30°C and cultured at 150r / min for 5 days, and samples were...
Embodiment 3
[0027] Degradation performance of lambda-cyhalothrin in soil by P.
[0028] The soil samples used in this example were taken from farmland soil not polluted by lambda-cyhalothrin. The soil samples were crushed and passed through a 100-mesh sieve, and placed in a ventilated place to dry for later use. Add lambda-cyhalothrin standard solution to the inorganic salt medium to make the concentration 100mg / L, take the above-mentioned inorganic salt culture solution, and evenly spray it into the prepared soil sample, so that the lambda-cyhalothrin in the soil The concentration is 100 mg / kg, and the soil sample is divided into 6 parts. Take 3 prepared soil samples and add the prepared Paleobacterium intermedia suspension (OD 600 =1.0), so that the soil sample contains 100mL / kg of degrading bacteria agent, fully mix well, set up three repeated experiments, with three parts of soil samples that do not add bacteria but add the same volume of sterile water as the blank control of abioti...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More