Pretreatment method for rapidly extracting lead and cadmium in grain sample
A technology for samples and grains, applied in the field of pretreatment, can solve the problems of increasing the cost of pretreatment and the high price of antibodies, and achieve the effects of low price, high extraction accuracy and good application value.
Pending Publication Date: 2021-08-06
BEIJING MEIZHENG BIOLOGICAL TECH +1
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Embodiment 1
[0022] 1. Pretreatment of grain samples
[0023] The negative rice and wheat samples were ground and crushed, and passed through a 40-mesh sieve as experimental samples. Weigh 2g ± 0.02g of rice sample and wheat sample respectively, and the treatment method of each sample is as follows: add 7mL of 0.5M hydrochloric acid as the extraction solution, shake for 3min, centrifuge at 4000r / min for 3min, then take 4mL of supernatant, pass Solid phase extraction column, elute the column with 1mL nitric acid solution, collect the effluent, neutralize the pH to 6-8 with 1M sodium hydroxide, take this neutralization solution 200μL+200μL 2%EDTA-2Na solution to form EDTA-pb or EDTA-Cd solution, this mixture is directly tested with lead or cadmium quick detection test strips (the test strips are commercially available products in this announcement), and then use a portable scanning reader to measure the value of the detection line, according to the pre-made The standard curve of heavy metal...
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Abstract
The invention discloses a pretreatment method for rapidly extracting lead and cadmium in a grain sample, which comprises the following steps: (1) adding an extracting solution into the grain sample, oscillating and mixing, and then centrifugally collecting supernate, wherein the extracting solution is a hydrochloric acid solution; (2) enabling the supernate extracted in the step (1) to pass through a solid-phase extraction column, then eluting the column with an acid solution, and collecting eluent; the preparation method of the extraction filler used by the solid-phase extraction column comprises the following steps: dissolving A336 in trichloromethane, then adding a KS-C18 filler for mixing, and then carrying out rotary evaporation on trichloromethane in a fuming cupboard to dryness to form C18 filler powder loaded with trioctyl methyl ammonium chloride; and (3) neutralizing the eluent with an alkaline solution until the pH value is 6-8, and adding an EDTA-2Na solution to form an EDTA metal chelate which is directly used for immunological detection. The pretreatment method disclosed by the invention is simple and convenient to operate, low in price and high in extraction accuracy, and has a good application value.
Description
technical field [0001] The invention relates to a pretreatment method, in particular to a pretreatment method for rapidly extracting lead and cadmium in grain samples. Background technique [0002] Food is the basis for the survival and development of human society, and an essential consumable for life-sustaining activities. In recent years, with the rapid development of my country's social economy, water pollution, air pollution, and soil pollution have become increasingly serious, posing a serious threat to food security. In particular, heavy metal pollution of food, heavy metal pollution incidents such as "arsenic poisoning" and "cadmium rice" have become representatives of the most concerned public health incidents. The heavy metals left in the environment enter the human body through the food chain and cause chronic damage to the body through the accumulation effect, which in turn causes physiological deformities, cancer and other refractory symptoms. At the same time...
Claims
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Login to View More IPC IPC(8): G01N1/28G01N33/531
CPCG01N1/28G01N33/531
Inventor 赵小旭
Owner BEIJING MEIZHENG BIOLOGICAL TECH