Method for detecting amantadine and rimantadine in tomatoes

Abstract

The invention belongs to the technical field of substance detection, and particularly provides a method for detecting amantadine and rimantadine in tomatoes. According to the method, a PSA adsorbent, a C18 adsorbent and anhydrous magnesium sulfate are adopted as purification reagents, impurities which interfere with a target object in an extract solution can be adsorbed, the technical problem that matrix interference is serious is solved, and the detection accuracy is improved; and by adopting liquid chromatography-tandem mass spectrometry detection, the sensitivity of the detection method is greatly improved. The detection method provided by the invention is less in used solvent and low in cost. Furthermore, the accuracy, the precision and the sensitivity of the detection method are improved by further limiting liquid chromatography-tandem mass spectrometry detection parameters.

Description

technical field [0001] The invention relates to the technical field of substance detection, in particular to a method for detecting amantadine and rimantadine in tomato. Background technique [0002] Amantadine (AMT) is an amino derivative of saturated tricyclodecane, also known as amantadine, tricyclodecane, etc., and was synthesized in 1960. It was first used as an antiviral drug and was the first to be approved by the FDA. anti-influenza drugs. Rimantadine (RMT) is a drug obtained by replacing the amino group of amantadine with an ethylamine group. This drug was approved by the United States in 1993 as an antiviral drug for the prevention and treatment of influenza A virus. Most of the amantadine and rimantadine used in the breeding process are excreted with feces and urine, resulting in high levels of antibiotics (such as amantadine and rimantadine) in the feces. Farming waste in our country is becoming an important source of antibiotics in the environment and has attr...

AI Technical Summary

Problems solved by technology

The literature reports are all methods for the determination of amantadine and rimantadine in food of animal origin; the determination methods mainly include gas chromatography, high performance liquid chromatography, capillary electrophoresis and liquid chromatography-mass spectrometry, and the above determination methods have low sensitivity

The pretreatment method mostly adopts SPE solid phase extraction column purification method, the above pretreatment method is cumbersome to operate and has a lot of matrix interference

At the same time, there are no reports on the detection methods of amantadine and rimantadine residues in plant-derived foods, especially tomatoes.

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