Microbial fuel cell for monitoring number of microorganisms and monitoring system and method

A microbial count and fuel cell technology, applied in biochemical fuel cells, fuel cells, battery electrodes, etc., can solve problems such as the inability to monitor the microbial count, and achieve faster monitoring speed and accuracy, low cost, and improved stability. effect of life

Active Publication Date: 2021-08-10
CENT SOUTH UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microbial fuel cells can send a signal of current change through the mechanism of electron transfer generated by the interaction between microorganisms and pollutants. So far, the microbial fuel cell sensors studied can be used for monitoring BOD, external temperature, pH changes, water quality, etc., but cannot realize Microbial Count Monitoring

Method used

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  • Microbial fuel cell for monitoring number of microorganisms and monitoring system and method

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Effect test

Embodiment 1

[0031] A microbial fuel cell for treating nitrate and capable of monitoring the number of microorganisms. The microbial fuel cell is composed of a cell body, an anode, and a cathode. The cell body is provided with a cathode and an anode, and a neutral nitrate solution and an active Sludge and activated sludge are taken from the anaerobic tank of the domestic sewage treatment plant. The basic parameters of the sludge are as described in Table 1, and the volume ratio of activated sludge to solution is 1:1.

[0032] Table 1 The basic parameters of the sludge used

[0033] parameter value TCOD (total chemical oxygen demand) (mg / L) 13000 SCOD (soluble chemical oxygen demand) (mg / L) 600 TSS (total suspended solids) (g / L) 20 VSS (Volatile Suspended Solids) (g / L) 15

[0034] Both the cathode and the anode float on the buffer solution, and the solution used for the reaction is composed of a concentration of 0.01 mol L - 1 NaNO 3 made, and adde...

Embodiment 2

[0047] In the pool body of the microbial fuel cell, add an inoculant equivalent to 2% of the total mass of rice husk and sludge, mix it evenly and heat it with a heating mantle at 50°C for 4 hours to ensure the full activation of the inoculum, and then use the blood cell counting method to Count the number of microorganisms under the microscope, then connect the microbial fuel cell and each unit and module, start the microbial fuel cell, and collect the current value and compare it with the standard curve after the operation is stable. The measurement results show that the number of microorganisms is 1.332×10 6 , so the measurement deviation is 4.0%.

Embodiment 3

[0049] In the pool body of the microbial fuel cell, add an inoculant equivalent to 2% of the total mass of rice husk and sludge, mix it evenly and heat it in a heating mantle at 60°C for 4 hours to ensure the full activation of the inoculum, and then use the blood cell counting method to Count the number of microorganisms under the microscope, then connect the microbial fuel cell and each unit and module, start the microbial fuel cell, and collect the current value and compare it with the standard curve after the operation is stable. The measurement results show that the number of microorganisms is 1.098×10 6 , so the measurement deviation is 3.0%.

[0050] According to the above-mentioned embodiments, the monitoring system of the present application can quickly detect the number of microorganisms in the microbial fuel cell, and the measurement deviation is small, which can effectively solve the problem of when to add microorganisms and the number of microorganisms in practica...

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Abstract

The invention belongs to the field of water quality monitoring, and particularly relates to a microbial fuel cell for monitoring the number of microorganisms and a monitoring system and method. The microbial fuel cell comprises a cell body, an anode, a cathode and an electrolyte, the anode and the cathode are pretreated graphite felt circles; and the electrolyte is formed by mixing neutral nitrate and activated sludge according to the volume ratio of (1-2): (1-2). According to the invention, through coupling of the electrodes, a signal processing unit and a wireless transmission module, the sensitivity of the electrodes and the monitoring efficiency of the quantity of residual microorganisms in the water body are improved; and the system generally realizes intelligence and automation of microbe quantity monitoring, and has the advantages of convenience in operation, high efficiency, low cost and the like.

Description

technical field [0001] The invention belongs to the field of water quality monitoring, and in particular relates to a microbial fuel cell, a monitoring system and a method for monitoring the number of microorganisms. Background technique [0002] Water resources are one of the essential resources for human life. With the growth and development of social economy and life, the demand for water resources is increasing. The total amount of water resources on the earth is about 1.4×10 9 km3, of which fresh water resources only account for 2.7%, and the available amount is less than 1%, so fresh water resources are extremely precious. The water required for human activities comes from natural water bodies, and the resulting wastewater is discharged into the water bodies, causing them to be polluted to varying degrees. There are many types of pollutants in water bodies, including solid pollutants, organic pollutants, toxic pollutants, and inorganic pollutants. One of the most co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01M4/96H01M8/04537H01M8/16
CPCH01M8/16H01M8/04582H01M4/96H01M2300/0091Y02E60/50
Inventor 常兴华金胜明白泽惠
Owner CENT SOUTH UNIV
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