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Method for extracting Sanzan gum from fermentation liquor and product thereof

A technology of fermented liquid and sanzan gum, which is applied in the field of extracting sanzan gum, can solve the problems that the number of microorganisms in the product is difficult to control, does not have the value of scale-up production application, and cannot realize industrial production, etc., and achieves high production scalability and low production cost Low, stable whiteness effect

Active Publication Date: 2021-08-13
MEIHUA BIOTECH LANGFANG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method is only suitable for the preparation of small quantities of products in the laboratory, cannot realize industrial production, and does not have the application value of scale-up production, so it has not been widely used
[0007] To sum up, there are still some shortcomings such as low viscosity and gel strength in the extraction process of the product, and it is difficult to control the number of microorganisms in the product.
At the same time, in the prior art, alkaline substances are used to call back the separated fibrous material to form a paste material, which is then dried and pulverized. The pH adjustment process of this material requires high equipment, high power and Stirring equipment with good effect, otherwise the pH adjustment process will form uneven materials and affect product quality
Due to the high moisture content, the requirements for drying equipment are also high, and infrared drying is often required. Compared with conventional drying methods, this equipment has defects such as high investment cost and high operation and maintenance costs.

Method used

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  • Method for extracting Sanzan gum from fermentation liquor and product thereof
  • Method for extracting Sanzan gum from fermentation liquor and product thereof
  • Method for extracting Sanzan gum from fermentation liquor and product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The present embodiment provides a kind of method for extracting Sanzan gum product of the present invention, comprises the following steps:

[0047] (1) Add sodium chloride equivalent to 0.9% (m / m) of the fermentation broth (in the form of an aqueous solution) to the fermentation broth, stir evenly and then let stand for 15 minutes.

[0048] (2) In the solution obtained in step (1), add a 70% (v / v) ethanol solution until the alcohol content in the raw material solution reaches 35±5% (v / v).

[0049] (3) Add 6 mol / L sulfuric acid solution to the solution obtained in step (2), and measure the pH of the material to 1.75 while stirring to obtain a sedimentation liquid with fiber materials.

[0050] (4) Separating the sedimentation liquid to obtain fibrous material agglomerates and mother liquor.

[0051] (5) Add 400 mL of 95% (v / v) ethanol solution to the 200 g mass of material obtained in step (4), stir to adjust the ethanol concentration in the material to 76.8% (v / v), an...

Embodiment 2

[0058] The present embodiment provides a kind of method for extracting Sanzan gum product of the present invention, specifically as follows:

[0059] (1) Add potassium chloride equivalent to 0.7% (m / m) of the fermentation broth (added in the form of aqueous solution) to the fermentation broth, stir evenly and then let it stand for 20 minutes.

[0060] (2) In the solution obtained in step (1), add a concentration of 85.2% (v / v) ethanol solution (obtained by the 9th step in the above-mentioned Example 1) to reach 35 ± 5% alcohol content in the raw material solution (v / v).

[0061] (3) Add 6 mol / L hydrochloric acid solution to the solution obtained in step (2), and measure the pH of the material to 1.58 while stirring to obtain a sedimentation liquid with fiber materials.

[0062] (4) Separating the sedimentation liquid to obtain fibrous material agglomerates and mother liquor.

[0063] (5) in the 200g material agglomerate that step (4) obtains, add the ethanol solution of 900m...

Embodiment 3

[0070] The present embodiment provides a kind of method for extracting Sanzan gum product of the present invention, specifically as follows:

[0071] (1) Add anhydrous calcium chloride equivalent to 0.5% (m / m) of the fermentation broth (added in the form of aqueous solution) to the fermentation broth, stir evenly and let stand for 15 minutes.

[0072] (2) In the solution obtained in step (1), adding a concentration of 85.2% (v / v) ethanol solution (obtained in the 9th step in the above-mentioned embodiment 1) reaches 35 ± 5% ( v / v).

[0073] (3) Add 6 mol / L hydrochloric acid solution to the solution obtained in step (2), and measure the pH of the material to 1.88 while stirring to obtain a sedimentation liquid with fiber materials.

[0074] (4) Separating the sedimentation liquid to obtain fibrous material agglomerates and mother liquor.

[0075] (5) in the 200g material agglomerate that step (4) obtains, add the ethanol solution of 950mL 85.2% (v / v) (the 9th step obtains in ...

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Abstract

The invention relates to the technical field of microbial polysaccharide extraction, and particularly discloses a method for extracting Sanzan gum from fermentation liquor and a product thereof. The method for extracting Sanzan gum from the fermentation liquor comprises the following steps: treating the fermentation liquor with neutral soluble salt, adding ethanol to enable the concentration of ethanol in the system to reach 30-40%, adjusting the pH value of the system to 1.0-3.0, carrying out separation operation, soaking the obtained crude product with ethanol to obtain a material system with the ethanol concentration of 72-85%, and then adjusting the pH value of the material system to 6.0-8.0, ensuring that the concentration of ethanol in the material system is 70-82%, and separating again. The product obtained by the invention has the advantages of high gel strength, high product viscosity, low total number of bacterial colonies, high product percent of pass and the like. Meanwhile, the obtained mother liquor can be recovered and reused after being distilled, so that the method has very high practicability and is beneficial to industrial production.

Description

technical field [0001] The invention relates to the technical field of microbial polysaccharide extraction, in particular to a method for extracting sanzan gum from fermentation liquid and its product. Background technique [0002] Sanzan gum is a kind of microbial polysaccharide, which belongs to biopolymer. The product is often used as a thickener, stabilizer and coagulant because of its high viscosity in aqueous solution, good thickening performance, good thermal gelation and gel thermal reversibility, and uniform suspension of solid particles in the final product. . This product is currently used in neutral vegetable protein drinks, acidic fruit and vegetable juice drinks, and meat enemas in the food field. For example, the patent CN108719730 discloses the application of Sanzan gum in suspension stabilizers. The suspension system prepared by using Sanzan gum has Good recoverability, after violent shaking or stirring during transportation, it can still return to the ori...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003Y02E50/10
Inventor 胡炎华于宏艳常利斌李岩
Owner MEIHUA BIOTECH LANGFANG CO LTD
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