Transfer breeding method of practical silkworm sericin cocoon strain based on SSR molecular markers

A molecular marker and practical technology, applied in biochemical equipment and methods, microbiological determination/inspection, animal husbandry, etc., can solve the problems of slow progress in breeding of practical sericin cocoon strains, genotype determination, etc., and achieve rapid and accurate selection The effects of breeding, improving yield and quality, and keeping traits are good

Pending Publication Date: 2021-08-13
ANKANG UNIV +1
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  • Abstract
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for the transfer of silkworm practical sericin cocoon strains based on SSR molecular markers, which solves the problem that the genotype cannot be determined by phenotype in the breeding process of silkworm practical sericin cocoon strains, which leads to the progress of practical sericin cocoon strain breeding. slow problem

Method used

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  • Transfer breeding method of practical silkworm sericin cocoon strain based on SSR molecular markers
  • Transfer breeding method of practical silkworm sericin cocoon strain based on SSR molecular markers
  • Transfer breeding method of practical silkworm sericin cocoon strain based on SSR molecular markers

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Embodiment

[0037] There are 3 silkworm varieties, namely the Chinese practical variety Chunfeng, the Japanese practical variety ZhenJ, and the silk fibroin heavy chain gene mutant variety Ser.

[0038] Chunfeng (middle line): bred by Economic Crops Research Institute of Hubei Academy of Agricultural Sciences. It has two hua and four sleep, large silk spot, thick body, oblong cocoon shape, white cocoon color, medium and thick shrinkage. The hatching is neat, the development is uniform, the sleep is fast, the sleep is up, the mating performance is excellent, the production is good, the eggs are less fertilized, and the egg quality is substantial.

[0039] Zhen J (Japanese): It was bred by the Economic Crops Research Institute of the Hubei Academy of Agricultural Sciences. It has two morphs and four sleeps. It has common spots on large silkworms. It has a thick body and a shallow cocoon. It hatches well, sleeps well, develops neatly, is strong and easy to raise, moths are well developed, ea...

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Abstract

The invention discloses a transfer breeding method of a practical silkworm sericin cocoon strain based on SSR molecular markers. The transfer breeding method comprises the following steps: hybridizing a female practical silkworm variety and a male fibroin heavy-chain gene mutation variety to obtain F1; backcrossing F1 female and practical variety male to obtain BC1; dividing the BC1 into a dominant homozygous type and a heterozygous type by using a polymorphic SSR molecular marker on a chromosome 25; backcrossing the heterozygous BC1 female and the practical variety male to obtain BC2; continuously backcrossing the heterozygous female of the backcross progeny with the practical variety male to BC5; by using polymorphic SSR molecular markers on 28 chromosomes, selecting BC5 individuals with 27 pairs of chromosomes derived from practical varieties except the chromosome 25; and obtaining the recessive homozygous individual in the BC5 selfing progeny, which is the practical sericin cocoon strain. According to the method, the practical silkworm variety is transfered into the practical sericin cocoon strain by utilizing the characteristic that female silkworm chromosomes are completely linked and polymorphic SSR molecular markers, the chromosome source is controllable in the whole breeding process, and accurate breeding can be realized.

Description

technical field [0001] The invention belongs to the technical field of silkworm breed selection and breeding, and in particular relates to a method for transferring silkworm practical sericin cocoon strains based on SSR molecular markers. Background technique [0002] Silk protein is a natural polymer material secreted by silkworms, mainly composed of silk fibroin and sericin. When the silkworm spins silk, one silk contains two juxtaposed silk fibers, and the outside is wrapped with sericin, which plays a role of fixation and adhesion. Sericin belongs to lamellar colloidal protein, which can bond silk fibers together to form a firm cocoon shell structure, which plays a role of protection and glue, and is beneficial to the growth and development of silkworm chrysalis. Due to different silkworm species, the proportion of sericin and silk fibroin is slightly different. In commercial silkworm species, sericin usually accounts for about 25%-30% of the total weight of silk, silk...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888A01K67/04
CPCC12Q1/6888A01K67/04C12Q2600/156C12Q2600/124A01K2217/15A01K2227/706A01K2267/02
Inventor 陈安利吴凡彭云武孟刚凌君
Owner ANKANG UNIV
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