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A kind of anti-aging cell preparation and preparation method thereof

A cell preparation and anti-aging technology, applied in the field of biomedicine, can solve the problems of limited number of cell sources, complicated production, unfavorable application, etc., and achieve the effects of shortening production time, simplifying production procedures, and prolonging lifespan

Active Publication Date: 2022-02-22
广东莱迪生物医药研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are currently anti-aging studies using bone marrow and umbilical cord mesenchymal stem cells, but the sources of these cells are limited, and the production is complicated and time-consuming, which is not conducive to clinical application

Method used

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  • A kind of anti-aging cell preparation and preparation method thereof
  • A kind of anti-aging cell preparation and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0023] 1. Isolation of peripheral blood mononuclear cells

[0024] 1. Take 200ml of peripheral blood from healthy young people under the age of 30 each time (normal blood and urine routine, normal cardiopulmonary function, no genetic diseases, no hepatitis B, AIDS, leptospirosis and other infectious diseases);

[0025] 2. Use human mononuclear cell separation medium (Tianjin Haoyang Huake Biotechnology Co., Ltd.), and centrifuge at 2000rpm for 20min to separate the mononuclear cell population;

[0026] 3. After counting, proceed to the next step of cultivation.

[0027] 2. Pre-induction culture and identification of peripheral blood mononuclear cells

[0028] 1. Induced cell culture medium: DMEM / F12 (100ml / 100ml), LG ((mass fraction 3% L-glutamine solution, 100×, 1ml / 100ml), non-essential amino acid (100×, 1ml / 100ml), Recombinant human basic fibroblast growth factor rhbFGF (2000ng / 100ml), recombinant human epidermal growth factor rhEGF (2000ng / 100ml), recombinant human stem ...

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Abstract

The invention discloses an anti-aging cell preparation and a preparation method thereof. The peripheral blood of healthy young people is extracted, the mononuclear cell population is separated, and then the mononuclear cells are cultured with an induced cell culture medium to obtain an anti-aging cell preparation; the induced cell culture medium is added with DMEM / F12 basal medium L‑glutamine, non-essential amino acids, rhbFGF, rhEGF, rhSCF, rhBDNF, and human serum albumin. Peripheral blood mononuclear cells are isolated in vitro, and then pre-induced and cultured to remove peripheral blood terminal cells and obtain a cell population with peripheral blood stem / progenitor cell characteristics that is prone to neural stem cell differentiation. Such cells Allogeneic input into elderly frail individuals can not only enhance the physical fitness of the frail elderly individuals, but also increase the frail elderly individuals' perception of the surrounding environment, as well as increase the brightness and youthfulness of the skin. Animal experiments have shown that such human cells can prolong life after pre-induction culture.

Description

Technical field: [0001] The invention belongs to the field of biomedicine, and in particular relates to an anti-aging cell preparation and a preparation method thereof. Background technique: [0002] With the development of my country's economy, people's life expectancy is getting longer and longer. At present, the population over the age of 60 in my country has reached about 250 million, and it is expected to reach about 500 million in 2050. Therefore, our country has entered an aging society, and the issue of old-age care has become the focus of society. This includes the treatment of geriatric diseases, the improvement of the quality of life of the elderly, and the prolongation of life expectancy. Although the improvement of the current level of medicine and diet can solve some of the problems of the elderly, it still falls short of people's expectations. And cell therapy especially stem cell therapy has added a new hope to people's anti-aging. [0003] As we all know...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/02
CPCA61K35/14A61K35/15A61P39/06A61P39/00C12N5/0634C12N2500/32C12N2501/115C12N2501/11C12N2501/125C12N2501/13C12N2509/00C12N2501/998
Inventor 黄冰袁友朋
Owner 广东莱迪生物医药研究院有限公司
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