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Anti-aging cellular preparation and preparation method therefor

A cell preparation and anti-aging technology, applied in the field of biomedicine, can solve the problems of limited number of cell sources, unfavorable application, complicated production, etc., and achieve the effects of shortening production time, prolonging life, and simplifying production procedures

Active Publication Date: 2021-08-20
广东莱迪生物医药研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are currently anti-aging studies using bone marrow and umbilical cord mesenchymal stem cells, but the sources of these cells are limited, and the production is complicated and time-consuming, which is not conducive to clinical application

Method used

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  • Anti-aging cellular preparation and preparation method therefor

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Experimental program
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Effect test

Embodiment 1

[0023] 1. Isolation of peripheral blood mononuclear cells

[0024] 1. Take 200ml of peripheral blood from healthy young people under the age of 30 each time (normal blood and urine routine, normal cardiopulmonary function, no genetic diseases, no hepatitis B, AIDS, leptospirosis and other infectious diseases);

[0025] 2. Use human mononuclear cell separation medium (Tianjin Haoyang Huake Biotechnology Co., Ltd.), and centrifuge at 2000rpm for 20min to separate the mononuclear cell population;

[0026] 3. After counting, proceed to the next step of cultivation.

[0027] 2. Pre-induction culture and identification of peripheral blood mononuclear cells

[0028] 1. Induced cell culture medium: DMEM / F12 (100ml / 100ml), LG ((mass fraction 3% L-glutamine solution, 100×, 1ml / 100ml), non-essential amino acid (100×, 1ml / 100ml), Recombinant human basic fibroblast growth factor rhbFGF (2000ng / 100ml), recombinant human epidermal growth factor rhEGF (2000ng / 100ml), recombinant human stem ...

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Abstract

The invention discloses an anti-aging cellular preparation and a preparation method therefor. The anti-aging cellular preparation is prepared through extracting peripheral blood from a healthy youngster, separating a single karyocyte colony from the peripheral blood, and then, culturing single karyocytes with a cell-induced culture medium; and according to the cell-induced culture medium, L-glutamine, non-essential amino acids, rhbFGF, rhEGF, rhSCF, rhBDNF and human serum albumin are added into a DMEM / F12 basal culture medium. The single karyocytes are separated from the peripheral blood in vitro and then are subjected to pre-induced culture, thus, terminal cells can be removed from the peripheral blood, and a cell population with neural stem cell differentiation trend and peripheral blood stem / progenitor cell characteristics can also be obtained; and by inputting such cellular variants to elderly weak individuals, the constitution of the weak elderly individuals can be strengthened, and the surrounding environment perceptibility, skin brightness and youngness of the weak elderly individuals can also be improved. Animal experiments show that such pre-induced-cultured human cells can be used for prolonging lives.

Description

Technical field: [0001] The invention belongs to the field of biomedicine, and in particular relates to an anti-aging cell preparation and a preparation method thereof. Background technique: [0002] With the development of my country's economy, people's life expectancy is getting longer and longer. At present, the population over the age of 60 in my country has reached about 250 million, and it is expected to reach about 500 million in 2050. Therefore, our country has entered an aging society, and the issue of old-age care has become the focus of society. This includes the treatment of geriatric diseases, the improvement of the quality of life of the elderly, and the prolongation of life expectancy. Although the improvement of the current level of medicine and diet can solve some of the problems of the elderly, it still falls short of people's expectations. And cell therapy especially stem cell therapy has added a new hope to people's anti-aging. [0003] As we all know...

Claims

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Application Information

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IPC IPC(8): A61K35/14A61K35/15A61P39/06A61P39/00C12N5/078
CPCA61K35/14A61K35/15A61P39/06A61P39/00C12N5/0634C12N2500/32C12N2501/115C12N2501/11C12N2501/125C12N2501/13C12N2509/00C12N2501/998
Inventor 黄冰袁友朋
Owner 广东莱迪生物医药研究院有限公司
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