Preparation method of gourd seed oil for assisting in reducing blood fat

A technology for assisting blood fat reduction and gourd seeds, which is applied in the direction of fat oil/fat production, fatty acid preparation/refining, fat production, etc. It can solve the problems that there is no preparation method of gourd seed oil fatty acid products, so as to improve the content of linolenic acid and nutritional value high effect

Pending Publication Date: 2021-08-20
乌鲁木齐市中医医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no gourd seed oil fatty acid product and its preparation method that can be used to assist in reducing blood lipids, etc.

Method used

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  • Preparation method of gourd seed oil for assisting in reducing blood fat
  • Preparation method of gourd seed oil for assisting in reducing blood fat
  • Preparation method of gourd seed oil for assisting in reducing blood fat

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preparation example Construction

[0020] A preparation method of gourd seed oil for assisting blood fat reduction, comprising the following steps:

[0021] a) using gourd seed powder as raw material, extracting the crude fat of gourd seeds by Soxhlet extraction;

[0022] b) adding ethanol to the crude fat of gourd seeds, and catalyzing the synthesis by immobilized lipase Lipozyme TL IM to obtain fatty acid ethyl esters of gourd seed oil with a conversion rate of more than 80%;

[0023] c) the obtained gourd seed oil fatty acid ethyl ester is obtained through urea and has a purity of more than 95% unsaturated fatty acid;

[0024] In step a), the crude fat oil content of the gourd seeds reaches 40-50%, including 55-58% linoleic acid and 8-10% oleic acid;

[0025] In step b), the conversion rate of fatty acid ethyl ester of gourd seed oil reaches more than 85%, wherein linoleic acid is 78-81%, oleic acid is 10-12%;

[0026] In step c), the urea-included gourd seed oil fatty acid has a purity of more than 95%, w...

Embodiment 1

[0035] A preparation method of gourd seed oil for assisting blood fat reduction, comprising the following steps:

[0036] a) Get 10.0g of gourd seed powder, adopt Soxhlet extraction method to extract gourd seed crude fat, its oil content reaches 52%, wherein linoleic acid 56%, oleic acid 9%;

[0037] b) Add the crude fat of gourd seeds to ethanol (alcohol-oil molar ratio is 4:1), the mass fraction of lipase Lipozyme TL IM is 30%, the reaction temperature is 50°C, and the reaction time is 23 h; after immobilized lipase Lipozyme TL IM catalytic synthesis obtained a conversion rate of 85.8%, including 78% of linoleic acid and 10.5% of oleic acid;

[0038] c) Add urea (mass ratio: 1:3) to the obtained fatty acid ethyl ester of gourd seed oil, stir for 0.5 h, and clathrate at 3°C ​​for 28 h to obtain unsaturated fatty acids with a purity of 95.2%.

Embodiment 2

[0040] A preparation method for cucurbit seed oil for assisting blood fat reduction, comprising the following steps:

[0041] a) Get 10.0g of gourd seed powder, adopt Soxhlet extraction method to extract gourd seed crude fat, the oil content reaches 55%, wherein linoleic acid 58%, oleic acid 10%;

[0042] b) The crude fat of gourd seeds was added to ethanol (the molar ratio of alcohol to oil was 4: 1), the mass fraction of lipase Lipozyme TL IM was 32%, the reaction temperature was 53°C, and the reaction time was 24 h; after immobilized lipase Lipozyme TL IM catalytic synthesis obtained a conversion rate of 86.3%, including 79% of linoleic acid and 11% of oleic acid;

[0043] c) Add urea (mass ratio: 1:3) to the obtained gourd seed oil fatty acid ethyl ester, stir for 1 hour, and perform clathrate at 0°C for 24 hours to obtain unsaturated fatty acids with a purity of 97%.

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Abstract

The invention discloses a preparation method of gourd seed oil for assisting in reducing blood fat. The preparation method comprises the following steps: a) taking gourd seed powder as a raw material; b) adding cucurbit seed crude fat into ethanol (the molar ratio of oleyl to alcohol is 4: 1), adding immobilized lipase Lipozyme TL IM (the mass fraction of lipase is 30-32%), and performing catalytic synthesis under the conditions that the reaction temperature is 50-55 DEG C and the reaction time is 22-25 hours to obtain cucurbit seed oil fatty acid ethyl ester with the conversion rate of 80% or above; and c) adding urea (in a mass ratio of 1: 3) into the obtained cucurbit seed oil fatty acid ethyl ester, stirring for 0.5-1 hour, and clathrating at 0-5 DEG C for 24-36 hours to obtain the unsaturated fatty acid with the purity of 95% or above. The specific immobilized lipase Lipozyme TL IM is adopted for enzymolysis and urea inclusion, compared with a traditional method for extracting the gourd seed oil, the content of linolenic acid in the gourd seed oil can be remarkably increased, the nutritional value of the gourd seed oil is higher, and therefore the prepared gourd seed oil has the remarkable auxiliary blood fat reducing effect.

Description

technical field [0001] The invention relates to the field of food processing, in particular to a preparation method of gourd seed oil for assisting in lowering blood fat. Background technique [0002] Linolenic acid is an essential fatty acid, an omega-3 fatty acid containing three double bonds. Linolenic acid exists in dark green plants in the form of glyceride. It is the main component of human tissue cells and plays a decisive role in human health. It can improve and prevent diseases such as hyperlipidemia, hyperglycemia, and hypertension. Linolenic acid cannot be synthesized in the human body and can only be ingested from food. [0003] Gourd seeds and gourd seed oil are commonly used medicinal and edible materials in Uyghur medicine. At present, the gourd seeds are mainly directly crushed into powder, or the gourd seeds are pressed to extract oil for use. So far, there is no gourd seed oil fatty acid product and preparation method that can be used to assist in reduci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C11C1/02C11C1/04
CPCC11B1/10C11C1/02C11C1/045
Inventor 木太里普·吐逊刘艺李志辉尹辉库德热提·阿吉赵媛
Owner 乌鲁木齐市中医医院
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