Cocktail immunohistochemical kit for diagnosing breast cancer
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A technology of immunohistochemistry and kits, which is applied in the field of biomedicine, can solve the problems of poor repeatability of staining of tiny tissue samples, achieve the effect of reducing the amount of samples used, precious samples, and improving accuracy
Active Publication Date: 2021-08-24
河南赛诺特生物技术有限公司
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[0006] The purpose of the present invention is to provide a cocktail immunohistochemical kit for diagnosing breast cancer. The kit uses a cocktail to combine primary antibodies, and can simultaneously detect the expression of CK7, P63,
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Embodiment 1
[0039] Example 1 Cocktail Immunohistochemical Kit for Diagnosing Breast Cancer
[0040] A cocktail immunohistochemical kit for diagnosing breast cancer, comprising the following components:
[0042]Combined primary antibody: composed of pure water and the following components: mouse anti-human cytokeratin 7 monoclonal antibody (clone number: EP16, antibody titer: 1:200), mouse anti-human P63 monoclonal antibody (clone number: MyR1- P63, antibody titer: 1:200), rabbit anti-human MHC monoclonal antibody (clone number: SMMS-1, antibody titer: 1:150), 6g / L Tris, 10g / L The sodium chloride of bovine serum albumin, 8g / L, the chlorophyll of 0.2g / L, the Proclin950 that mass concentration is 0.15%; In other embodiments, mouse anti-human cytokeratin 7 monoclonal antibody titer is 1: 50-1:400, optional 1:50 or 1:400; mouse anti-human P63 monoclonal antibody titer 1:50-1:3...
Embodiment 2
[0050] Example 2 CNT330 automatic immunohistochemical staining (breast cancer tissue)
[0051] 1) Assemble the kit: Assemble the following components into a complete kit, including 20mL peroxidase blocking reagent, 20mL combined primary antibody, 20mL combined secondary antibody, 2mL fast red chromogenic reagent, 2mL fast red chromogenic activator , 35mL fast red buffer, 3mL DAB chromogenic substrate, 35mL DAB chromogenic buffer, 20mL hematoxylin counterstaining solution.
[0052] 2) Immunohistochemical test on breast cancer tissue
[0053] ①Take one breast cancer tissue, prepare 3 paraffin tissue sections with a thickness of 3 microns, bake the slices at 65°C for 1 hour, and mark them as A1, A2, and A3 respectively.
[0054] ②Put the kit of the present invention into a fully automatic immunohistochemical staining machine for identification, set the case number and section number, and put the section into the machine to be started.
[0055] ③ Dewaxing, antigen retrieval, imm...
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Abstract
The invention relates to a cocktail immunohistochemical kit for diagnosing breast cancer, which comprises a peroxidase sealing agent, a combined primary antibody, a combined secondary antibody, a DAB chromogenic substrate, a DAB chromogenic buffer solution, a fast red chromogenic agent, a fast red chromogenic activator, a fast red buffer solution and a hematoxylin restaining solution, wherein the combined primary antibody comprises a mouse anti-human cytokeratin 7 monoclonal antibody, a mouse anti-human P63 monoclonal antibody and a rabbit anti-human MHC monoclonal antibody; the combined secondary antibody comprises an anti-mouse secondary antibody polymer marked by HRP and an anti-rabbit secondary antibody polymer marked by AP. According to the kit, the cocktail combined primary antibody is adopted, the expression conditions of three antigen targets CK7, P63 and MHC can be detected on the same tissue slice at the same time, the use amount of samples is reduced, the difference caused by different slices is avoided, the problem of poor dyeing repeatability of tiny tissue samples is solved, normal tissue and cancer tissue can be visually and clearly distinguished, a more comprehensive and reliable diagnosis basis is provided for pathologists, and the diagnosis accuracy is improved.
Description
technical field [0001] The invention belongs to the field of biomedicine, in particular to a cocktail immunohistochemical kit for diagnosing breast cancer. Background technique [0002] Breast cancer is the most common malignant tumor among women in the world. According to statistics in 2018, the incidence and mortality of breast cancer in women worldwide are 4.63‰ and 1.3‰, respectively, showing an increasing trend year by year. Due to the population base in my country, the number of incidences and deaths of female breast cancer in China ranks first in the world. Therefore, early diagnosis, early treatment and prevention of breast cancer have become the focus of cancer control in my country. [0003] Many studies have shown that the occurrence and development of breast cancer is a multi-step process, first from normal epithelial hyperplasia to atypical hyperplasia to carcinoma in situ, then to invasive carcinoma, and finally to metastatic carcinoma. A very obvious feature...
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