Cocktail immunohistochemical kit for diagnosing breast cancer

Abstract

The invention relates to a cocktail immunohistochemical kit for diagnosing breast cancer, which comprises a peroxidase sealing agent, a combined primary antibody, a combined secondary antibody, a DAB chromogenic substrate, a DAB chromogenic buffer solution, a fast red chromogenic agent, a fast red chromogenic activator, a fast red buffer solution and a hematoxylin restaining solution, wherein the combined primary antibody comprises a mouse anti-human cytokeratin 7 monoclonal antibody, a mouse anti-human P63 monoclonal antibody and a rabbit anti-human MHC monoclonal antibody; the combined secondary antibody comprises an anti-mouse secondary antibody polymer marked by HRP and an anti-rabbit secondary antibody polymer marked by AP. According to the kit, the cocktail combined primary antibody is adopted, the expression conditions of three antigen targets CK7, P63 and MHC can be detected on the same tissue slice at the same time, the use amount of samples is reduced, the difference caused by different slices is avoided, the problem of poor dyeing repeatability of tiny tissue samples is solved, normal tissue and cancer tissue can be visually and clearly distinguished, a more comprehensive and reliable diagnosis basis is provided for pathologists, and the diagnosis accuracy is improved.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a cocktail immunohistochemical kit for diagnosing breast cancer. Background technique [0002] Breast cancer is the most common malignant tumor among women in the world. According to statistics in 2018, the incidence and mortality of breast cancer in women worldwide are 4.63‰ and 1.3‰, respectively, showing an increasing trend year by year. Due to the population base in my country, the number of incidences and deaths of female breast cancer in China ranks first in the world. Therefore, early diagnosis, early treatment and prevention of breast cancer have become the focus of cancer control in my country. [0003] Many studies have shown that the occurrence and development of breast cancer is a multi-step process, first from normal epithelial hyperplasia to atypical hyperplasia to carcinoma in situ, then to invasive carcinoma, and finally to metastatic carcinoma. A very obvious feature...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a cocktail immunohistochemical kit for diagnosing breast cancer. The kit uses a cocktail to combine primary antibodies, and can simultaneously detect the expression of CK7, P63, and MHC three antigen targets on the same tissue section. In order to provide more diagnostic information for pathologists, improve the accuracy of diagnosis, and solve the problem of poor repeatability of dyeing micro-tissue samples

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