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Method for inducing somatic embryogenesis by using rubber tree calyx

A technology for somatic embryos and cell embryos is applied in the field of inducing somatic embryogenesis in rubber tree calyx, which can solve the problems of high operator requirements, low sampling success rate, difficult operation, etc., and achieves reduction of technical requirements and enrichment of fast propagation methods. , the effect of simplifying the acquisition steps

Active Publication Date: 2021-09-03
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, in the actual application process, using stamens to induce somatic embryogenesis in Hevea hevea needs to use dissecting needles and tweezers to peel off the calyx of male flowers and pick out the stamens. Because the stamens are only 1-2mm small, the operation is difficult and the success rate of sampling is not high. And the sampling is limited by the pollen development period. In addition, the stamens of many excellent varieties of rubber trees are aborted, only flower buds are formed, and stamens suitable for in vitro culture cannot be formed.
However, using the technology of integument-induced rubber tree somatic embryogenesis, because of the luxuriant branches and leaves of the rubber tree in the young fruit stage, it is difficult to distinguish and collect the young fruit in the young stage, which requires high requirements for operators, and many excellent varieties of rubber trees are available all year round. fruitless
In addition, because stamens contain haploid pollen, and young fruit contains fertilized zygotic embryos and triploid endosperm, the callus and somatic embryos induced by using stamens and inner integuments as explants have non-somatic origin. risk

Method used

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  • Method for inducing somatic embryogenesis by using rubber tree calyx
  • Method for inducing somatic embryogenesis by using rubber tree calyx
  • Method for inducing somatic embryogenesis by using rubber tree calyx

Examples

Experimental program
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Effect test

Embodiment 1

[0033] On sunny mornings for more than 2 consecutive days, select a mother plant that grows vigorously and is free from diseases and insect pests, collect immature inflorescences, wash them with running water for 5 minutes, then cut the inflorescences into inflorescence sprigs that are easy to disinfect, rinse them again with running water, and place them in an ultra- clean bench. First use 0.05% carbendazim for surface disinfection for 3 minutes, then use 75% alcohol for 30 seconds, then immediately disinfect with 0.1% mercuric chloride for 5 minutes, and finally rinse with sterile water for 5 times. 3 minutes each time.

[0034]Use tweezers and a scalpel to cut the flower bud explants from the inflorescence twigs as explants, each explant includes 3 flower buds, and the explants must be covered with calyx. Inoculate on the callus induction medium and culture for 6 weeks, the culture conditions are: temperature 27°C, relative humidity 65%, dark culture. During this process,...

Embodiment 2

[0039] On sunny mornings for more than 2 consecutive days, select a mother plant that grows vigorously and is free from diseases and insect pests, collect immature inflorescences, wash them with running water for 5 minutes, then cut the inflorescences into inflorescence sprigs that are easy to disinfect, rinse them again with running water, and place them in an ultra- clean bench. First use 0.05% carbendazim for surface disinfection for 3 minutes, then use 75% alcohol for 30 seconds, then immediately disinfect with 0.1% mercuric chloride for 5 minutes, and finally rinse with sterile water for 5 times. 3 minutes each time.

[0040] Use tweezers and a scalpel to cut off the bud explants from the inflorescence twigs as explants, each explant includes 0.5 flower buds, and the explants must be covered with calyx. Inoculate on the callus induction medium and culture for 6 weeks, the culture conditions are: temperature 27°C, relative humidity 75%, dark culture. During this process,...

Embodiment 3

[0045] On sunny mornings for more than 2 consecutive days, select a mother plant that grows vigorously and is free from diseases and insect pests, collect immature inflorescences, wash them with running water for 5 minutes, then cut the inflorescences into inflorescence sprigs that are easy to disinfect, rinse them again with running water, and place them in an ultra- clean bench. First use 0.05% carbendazim for surface disinfection for 3 minutes, then use 75% alcohol for 30 seconds, then immediately disinfect with 0.1% mercuric chloride for 5 minutes, and finally rinse with sterile water for 5 times. 3 minutes each time.

[0046] Use tweezers and a scalpel to cut the flower bud explants from the inflorescence twigs as explants, each explant includes 1 flower bud, and the explants must be covered with calyx. Inoculate on the callus induction medium and culture for 6 weeks, the culture conditions are: temperature 26°C, relative humidity 66%, dark culture. During this process,...

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Abstract

The invention provides a method for inducing somatic embryogenesis by using rubber tree calyx, and relates to the technical field of plant tissue culture. The method comprises the steps as follows: firstly, selecting immature inflorescences, cleaning and disinfecting the immature inflorescences, cutting tender flower buds to serve as explants, ensuring that each explant contains the flower buds, and covering the surfaces of the explants with calyxes; and inoculating the explants into a callus culture medium, forming an embryogenic callus from the calyx edge and the paraaxial surface, inoculating the callus into a somatic cell differentiation culture medium, and carrying out differentiation culture to obtain the somatic embryo. According to the method, the calyx is selected to induce the somatic embryogenesis, so that the defect that stamens or inner integuments induce the somatic embryogenesis is overcome, the rapid propagation method of rubber tree somatic embryo seedlings is enriched, in addition, the scheme is simple and reliable, and the collection difficulty and the requirements on operators are reduced.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for inducing somatic embryogenesis with rubber tree calyx. Background technique [0002] Rubber tree [Hevea brasiliensis (Willd.ex A.Juss.) Muell.Arg.] is a plant of the genus Hevea in the family Euphorbiaceae. It is a perennial tropical tall tree in the upper layer of the tropical rainforest. Its economic life is as high as 30-40 years. The latex it secretes is an important Moreover, the rubber tree is light in wood, beautiful in pattern and good in processing performance. After chemical treatment, it can be used to make high-grade furniture, fiberboard, plywood, pulp, etc., and has high economic value. [0003] The rubber tree is a panicle, born in the leaf axil or scale axil, monoecious, with different flowers in the same sequence; female flowers are born on the top of the flower branch, usually each inflorescence has 3-20 female flowers, and ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/00
Inventor 顾晓川彭素娜黄天带黄华孙徐正伟戴雪梅成镜李季周权男吴日智
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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