Stomach-small intestine-large intestine bionic digestion method for growing pigs and application of method to estimation of effective energy value of feed

Abstract

The invention discloses a method for simulating the stomach-small intestine-large intestine digestion process of growing pigs and application of the method to estimation of an effective energy value of feed. The method comprises the following steps: crushing and sieving a feed sample; preparing a stomach buffer solution, a small intestine buffer solution, a large intestine buffer solution, simulated gastric juice (containing pepsin), concentrated simulated small intestine juice (containing amylase, trypsin and chymotrypsin) and concentrated simulated large intestine juice (containing amylase, trypsin, chymotrypsin and cellulase); putting the crushed feed sample into a simulated digestive tube of a monogastric animal bionic digestive system, and respectively adding simulated gastric juice in sequence through computer program control to carry out stomach simulated digestion; adding concentrated simulated small intestine liquid for small intestine simulated digestion; adding concentrated simulated large intestine liquid for large intestine simulated digestion; and carrying out cleaning after digestion, and after undigested residues are obtained, measuring the total energy value of the residues and a feed sample, and calculating the effective energy value of the feed. The method overcomes the defect that in the traditional in-vitro simulated digestion method, only cellulase is contained in the large intestine digestion stage, but amylase, trypsin and chymotrypsin with certain active concentration are not contained in the large intestine liquid in the pig body, so that the measured value is closer to the measured values of digestible energy and metabolic energy of an animal test method, the correlation is higher, and the estimation deviation is lower.

Description

technical field [0001] The invention belongs to the field of animal husbandry, and relates to a method for simulating the digestion process of feed in the gastrointestinal tract of growing pigs, in particular to a method for rapidly estimating the effective energy value of feed by simulating the digestion process of the stomach-small intestine-large intestine of growing pigs in vitro. Background technique [0002] In the past 50 years, animal nutritionists have proposed many in vitro digestion models in simulating the digestion process of growing pig stomach-small intestine-large intestine, and tried to quickly estimate the effective energy value of pig feed through simulated digestion technology. Among them, the pepsin-pancreatin-fibrous carbohydrate enzyme established by Danish scholars Boisen and Fernandez in 1997 simulates the digestion process of growing pig stomach-small intestine-large intestine to estimate the effective energy value of pig feed, which has become the m...

AI Technical Summary

Problems solved by technology

The method of the present invention can solve the disadvantages that the activity of digestive enzymes in the simulated digestion of the small intestine is lower than that of the digestive enzymes in the small intestine in the body due to activity attenuation, and the amylase and protease activities in the simulated digestion of the large intestine are inconsistent with those of the large intestine in the body, and overcome the traditional in vitro digestion. The problem of large deviation and low correlation between the measured value of the method and the measured value of the animal test method

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