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Method for quantitatively analyzing microplastics in leaf vegetables

A quantitative analysis and microplastic technology, applied in the field of quantitative analysis of leafy vegetables pollutants, can solve the problems of oxidative damage, no quantitative analysis of microplastics, and damage to the structure of microplastics

Pending Publication Date: 2021-09-07
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] As of 2019, the total production of plastic products in my country has exceeded 1 billion tons, and it has become the world's largest plastic production and consumption country. However, the overall recycling rate of plastics is less than 20%, and a large amount of waste plastics enter plants. Current research shows that micro Plastics have a significant impact on the growth of a variety of plants. For example, it was observed by laser confocal microscopy that there are a large number of 0.1 μm microplastics in the root tip cells of faba bean, which obviously inhibited the normal growth of plants and caused their oxidative damage; Significant growth inhibitory effects were also observed in studies on the effects of plastic on wheat growth
However, most of the existing studies only elaborated the impact of microplastics on plant growth, but did not conduct quantitative analysis of microplastics in plants, which limited the research progress of microplastics absorption pathways, distribution rules and toxicity response mechanisms in plants.
[0003] At present, the quantitative analysis of bio-enrichment microplastics mainly focuses on the enrichment research in animals. For example, the patent CN201610045344.3 discloses a method for quantitative analysis of the enrichment and distribution of microplastics in mammals. This patent uses digestion and extraction methods , through the preparation of fluorescent microplastic particles, mammalian exposure experiments, sample collection, freeze-dried tissue samples, wet digestion samples, fluorescence quantitative detection of microplastic content and other steps to determine the content of microplastics enriched in mammals, but the invention of this application People have conducted a lot of research in the early stage, and the results show that because the structure and composition of leafy vegetable cells are quite different from animal cells, the microplastics in leafy vegetables are treated with the microplastic digesters of most animals such as fish. During digestion, the structure of microplastics will be destroyed, or the digestion of microplastics will not be complete, resulting in the final measurement of microplastic content in leafy vegetables is too high or too low, indicating that the digestion method for microplastics in mammals may not necessarily be able to Suitable for microplastics in leafy vegetables

Method used

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  • Method for quantitatively analyzing microplastics in leaf vegetables
  • Method for quantitatively analyzing microplastics in leaf vegetables
  • Method for quantitatively analyzing microplastics in leaf vegetables

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Hydroponics to obtain lettuce rich in microplastics

[0032] S1. Selection of microplastics: Polystyrene microplastic balls (microplastics) with a particle size of 0.2 μm purchased from Shanghai Huizhi Biotechnology Co., Ltd. were used as test materials. The material was wrapped with green fluorescent light at the center of the ball. The dye is not easily destroyed and quenched.

[0033] S2. Obtaining lettuce enriched with fluorescent microplastics: use 300mL Hoagland's solution as the hydroponic solution, carry out hydroponics to lettuce, and add polystyrene microplastic spheres with a concentration of 30mg / L to make Hoagland's The concentration of microplastics in the solution was 20 mg / L, and 6 parallel samples were set up; continuous culture was carried out for 10 days, the light intensity was kept at 15000 lux during the culture process, and the daytime temperature was kept at 22°C, and the nighttime temperature was kept at 16°C. Maintain the growth need...

Embodiment 2

[0034] Example 2 Quantitative analysis of lettuce microplastics by different digestion agents

[0035] 1. Experimental method:

[0036] S1. Put the lettuce after ultrasound and the leaves that fell off during the hydroponics process in an oven at 45°C to dry and grind to obtain 3.2 g of lettuce powder, and then take 10 mg of lettuce powder according to the composition of the digestion agent shown in Table 1 , volume, digestion temperature and digestion time, after mixing lettuce and digestion agent for digestion, the digestion solution was obtained (photographed figure 1 );

[0037] S2. Measure the fluorescence intensity of the digestion solution at a wavelength of 488nm with a NanoDrop 3300 ultra-micro-volume fluorescence spectrophotometer, and then calculate the concentration C of microplastics in the digestion solution according to the standard curve 2 , further calculation can obtain the content of microplastics in lettuce.

[0038] Table 1

[0039]

[0040] 2. Experi...

Embodiment 3

[0049] Example 3 A method for quantitative analysis of microplastics in lettuce

[0050] 1. Experimental method

[0051] S1. Put the ultrasonicated lettuce and the leaves that fell off during the hydroponics process in an oven at 45°C to dry and pulverize to obtain 3.2g of lettuce powder, then take 10mg of lettuce powder and mix it with 4mL of digestion agent at 50°C for digestion After 4h, the digestion solution was obtained;

[0052] S2. Measure the fluorescence intensity of the digestion solution at a wavelength of 488nm with a NanoDrop 3300 ultra-trace fluorescence spectrophotometer, then calculate the concentration of microplastics in the digestion solution according to the standard curve, and further calculate the content of microplastics in lettuce;

[0053] Wherein, the digestion agent consists of H 2 o 2 Aqueous solution and HNO 3 Aqueous solution composition; The HNO 3 The concentration of the aqueous solution is 65% (w / w), H 2 o 2 The concentration of the aqu...

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Abstract

The invention provides a method for quantitatively analyzing microplastics in leaf vegetables. The method comprises the following steps: crushing the leaf vegetables, mixing the crushed leaf vegetables with a digesting agent at 40-60 DEG C for 3-5 hours to obtain a digesting solution, measuring the concentration of the digesting solution, and calculating to obtain the content of the microplastics in the leaf vegetables. Through the specific digestion method suitable for the leaf vegetable body microplastics, the microplastics in the leaf vegetable body can be completely digested, and the structure of the microplastics is not damaged, so that the content of the microplastics in the leaf vegetable body is efficiently and accurately measured, and the method has considerable necessity for the technical field of quantitative analyzing of leaf vegetable pollutants.

Description

technical field [0001] The invention belongs to the technical field of quantitative analysis of leaf vegetable pollutants. More specifically, it relates to a method for quantitative analysis of microplastics in leafy vegetables. Background technique [0002] As of 2019, the total production of plastic products in my country has exceeded 1 billion tons, and it has become the world's largest plastic production and consumption country. However, the overall recycling rate of plastics is less than 20%, and a large amount of waste plastics enter plants. Current research shows that micro Plastics have a significant impact on the growth of a variety of plants. For example, it was observed by laser confocal microscopy that there are a large number of 0.1 μm microplastics in the root tip cells of faba bean, which obviously inhibited the normal growth of plants and caused their oxidative damage; Significant growth inhibitory effects were also observed in studies on the effects of plasti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/28
CPCG01N21/6402G01N1/28
Inventor 徐智敏郭勇军黄杏秀刘晖
Owner ZHONGKAI UNIV OF AGRI & ENG
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