Method for determining two beta-lactam antibacterial drug residues in milk

Abstract

The invention discloses a method for determining two beta-lactam antibacterial drug residues in milk, wherein the method comprises the steps: taking 1 mL of milk, putting into a 2 mL plastic centrifuge tube, gently adding 0.1 mL of trichloroacetic acid aqueous solution along the tube wall, carrying out vortex mixing for 30 seconds or more, centrifuging for 10 minutes at the speed of 6600 r / min, and sucking supernate into a 10 mL screw graduated test tube; then adding 0.2 mL of a trichloroacetic acid aqueous solution and 0.2 mL of a 7% formaldehyde aqueous solution, carrying out uniform vortex mixing for 30 seconds, covering and tightening, and heating in a water bath; and taking out, cooling to room temperature, fixing to 2 mL by using a 20% acetonitrile aqueous solution, uniformly mixing, filtering by using a 0.22 [mu]m microfiltration membrane, and determining and analyzing by using a high performance liquid chromatography. The method is accurate, reliable, high in recovery rate and good in reproducibility, the detection limit is lower than the highest veterinary drug residue detection limit (10 ng / mL) specified in China, the veterinary drug residue detection efficiency is improved, and theoretical basis and technical reference are provided for milk product enterprises and quality supervision departments for AMX and AMP drug multi-residue analysis.

Description

technical field [0001] The invention belongs to the field of food testing, in particular to the construction of an analysis method for two β-lactam antibacterial drugs in milk, that is, the residue analysis of amoxicillin (AMX) and ampicillin (AMP) in milk. Background technique [0002] β-lactam antibiotics are widely used to treat food-borne animal diseases, but long-term use or improper use will leave a large amount of penicillins in animals. For animals, drug resistance will occur in the body, which will affect the clinical efficacy of the drug. If people who are allergic to penicillins (Penicillins, PENs) eat foods with residual β-lactam antibacterial drugs, it will cause health hazards, which will destroy the normal flora environment in the human body and lead to the reduction of human immunity. For long-term consumption of foods with residual β-lactam antibacterial drugs, it is equivalent to long-term intake of low-dose antibiotics. The main hazards caused are: (1) m...

AI Technical Summary

Problems solved by technology

[0007] At present, there is no report on the analytical method for simultaneous detection of AMX and AMP residues in milk by pre-column derivatization HPLC UV method

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