Nucleic acid micro-damage detection method based on field effect transistor and biosensor

Abstract

The invention discloses a nucleic acid micro-damage detection method based on a field effect transistor and a biosensor. A liquid storage tank in which a solution is stored is fixed on a field effect transistor loaded with nucleic acid molecules, and a substance capable of damaging nucleic acid is added during detection, so that the substance interacts with the nucleic acid molecules in the field effect transistor, the configuration of the nucleic acid molecules is triggered to change, and the current change of the field effect transistor is further caused. The DNA micro-damage caused by drug molecules can be detected with high sensitivity. The method is a rapid and cheap DNA damage detection method.

Description

technical field [0001] The invention belongs to the field of bioelectronics and multifunctional sensing, and in particular relates to a field-effect transistor-based nucleic acid micro-damage detection method and a biosensor. Background technique [0002] DNA is damaged by endogenous metabolites, environmental carcinogens, and genotoxic chemotherapy drugs. These DNA damages can lead to gene mutation and chromosomal damage, which may lead to cancer and tumor growth in living organisms. However, in order to limit the instability of genes, DNA damage response pathways and repair proteins exist in living body cells to remove and alleviate DNA damage. Unrepaired DNA damage may act as a pathway to promote cell death, such as apoptosis and necrosis, which are also considered effective pathways for tumor suppression. By studying how cells replace DNA repair with cell death, researchers harness the killing effects of genotoxic chemotherapy drugs on tumor cells to treat cancer. How...

AI Technical Summary

Problems solved by technology

However, these methods require high doses of damaging agents and highly enriched nucleic acids to ensure signal amplification, which makes them unsuitable for in vivo detection at pM concentrations (Gietema, J.A., et al.Lancet 2000, 355, 1075-1076 .) The low incidence of DNA damage caused by ultra-low doses of drugs, so it is urgent to develop a highly sensitive method that can detect DNA damage caused by pM level gene-damaging drugs (such as cisplatin)

Images