Application of DADS in preparation of medicine for relieving intestinal flora disorder

A technology of intestinal flora and drugs, applied in the field of biomedicine, can solve problems to be studied and so on

Pending Publication Date: 2021-09-14
SHANGHAI TENTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

DADS in allicin has been reported to significantly inhibit CRC at the cell and animal levels, but the mechanism of inhibition of CRC in the field of microecology remains to be studied

Method used

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  • Application of DADS in preparation of medicine for relieving intestinal flora disorder
  • Application of DADS in preparation of medicine for relieving intestinal flora disorder
  • Application of DADS in preparation of medicine for relieving intestinal flora disorder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0120] This embodiment verifies that DADS inhibits the occurrence and development of mouse CRC and studies its mechanism. The specific experimental methods and results are as follows:

[0121] 1. Experimental method

[0122] 11 C57BL / 6J mice were divided into control group (Control group, n=5) and DADS intervention group (DADS group, n=6), CRC tumors were induced by AOM / DSS at week 5, and DADS The control group continued to use the DADS diet intervention, while the control group used the AIG diet intervention, and the body weight of the animals was recorded every week. At the 16th week, animal feces were collected for 16S rDNA detection and metabolomics detection. Afterwards, the animals were sacrificed, and the intestinal tissues of the animals were removed for evaluation of tumor size and number. Part of the intestinal tissue was collected and fixed with neutral fixative, and the other part of the intestinal tissue was collected into cryopreservation tubes and stored in a -...

Embodiment 2

[0153] In this example, pseudo-sterile mice were used to verify that DADS lost its inhibitory effect on mouse CRC after the antibiotics disturbed the flora. The specific experimental methods and results are as follows:

[0154] 1. Experimental method

[0155] Ten 7-week-old healthy male C57B / L6 mice were randomly divided into a pseudo-sterile control group (Control_PGF group, n=5) and a pseudo-sterile DADS intervention group (DADS_PGF group, n=5), and they were fed adaptively for one week. The control group ate the AIG feed, and the intervention group ate the DADS feed. In the first 4 weeks, quadruple antibiotics were added to the drinking water of the two groups to establish a pseudo-sterile animal model. In the 5th week, the two groups of mice were combined with AOM / DSS to induce the intestinal cancer model, and the diet intervention of the two groups was as described above. Animals were sacrificed at week 16, and tissues were collected for detection.

[0156] 2. Results ...

Embodiment 3

[0173] In this example, by constructing fecal bacteria transplantation group animals, it is reversely verified that DADS cannot inhibit CRC in the absence of normal intestinal flora structure. The specific experimental methods and results are as follows:

[0174] 1. Experimental method

[0175] 1.1 Preparation of bacteria solution for fecal transplantation

[0176] 1) Collect about 20g of feces from healthy C57BL / 6J mice within 3 hours;

[0177] 2) Use physiological saline to prepare 10% glycerin solution, grind and mix with feces at a ratio of 5:1 after sterilization;

[0178] 3) The suspension was filtered through 2.0mm, 1.0mm, 0.5mm, 0.25mm aperture screens respectively to obtain 200mg / ml bacterial liquid;

[0179] 4) Collect the bacterial liquid and freeze it at -80°C.

[0180] 1.2 Ten 7-week-old healthy male C57 B / L6 mice were randomly divided into fecal microbiota transplantation control group (Control_FMTgroup, n=5) and fecal microbiota transplantation allicin interv...

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Abstract

The invention provides application of DADS in preparation of a medicine for relieving intestinal flora disorder and application of DADS in preparation of a medicine for increasing short-chain fatty acid-producing flora in intestinal tracts of colorectal cancer patients. The effective component of the medicine is DADS. The DADS is added into a feed, and a CRC animal model is constructed, so that the DADS is found to relieve the intestinal flora disorder by increasing the short-chain fatty acid-producing flora and butyric acid in the intestinal tract, the colorectal cancer is further effectively prevented and treated, and a new strategy is provided for the preparation of related medicines. In addition, compared with other medicines for preventing and treating colorectal tumor, DADS is safer.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of DADS in the preparation of drugs for improving intestinal flora disturbance. Background technique [0002] Colorectal cancer (colorecta cancer, CRC) is a disease with the highest morbidity and mortality in the world and in China. It is one of the important reasons that affect the health of Chinese people. The curative effect is often unsatisfactory. In recent years, the incidence of CRC in China has been increasing year by year, and the long-term outcome of CRC patients is not ideal, with 429,000 new cases and 281,000 deaths each year. Even after successful surgical treatment, there are still complications such as long-term intestinal leakage, postoperative obstruction, and disease recurrence, which seriously affect the survival rate and quality of life of patients. Therefore, finding safe and effective preventive measures is a major clinical problem that needs to b...

Claims

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Application Information

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IPC IPC(8): A61K31/105A61P1/00
CPCA61K31/105A61P1/00
Inventor 秦环龙张扬朱烨飞孔程陈潜何继德尹芳吴夏维王楷若堵佳豪王欣月常勇亮
Owner SHANGHAI TENTH PEOPLES HOSPITAL
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