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A kind of preparation method of Bacillus preparation for promoting crop growth

A technique for growing bacillus and crops, which is applied in the direction of plant growth regulators, plant growth regulators, and methods based on microorganisms, which can solve the problems of high preparation costs and achieve benefits for crop growth, improve crop disease resistance, and prepare The effect of process stability

Active Publication Date: 2021-11-02
CHANGYI MINGXING FEED CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to make up for the high cost of preparation of plant growth-promoting bacteria preparations in the prior art, the present invention provides a method for preparing a bacillus preparation that promotes crop growth

Method used

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  • A kind of preparation method of Bacillus preparation for promoting crop growth
  • A kind of preparation method of Bacillus preparation for promoting crop growth
  • A kind of preparation method of Bacillus preparation for promoting crop growth

Examples

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Embodiment 1

[0018] Embodiment 1: A kind of preparation method of the bacillus preparation that promotes crop growth comprises the following steps: (1) enteromorpha powder is added into sterile deionized water according to mass volume ratio 1g: 50mL to obtain enteromorpha powder suspension, and above-mentioned Boil the Enteromorpha powder suspension for 30 minutes, adjust the pH value to 7.0 after cooling to room temperature, and obtain the Enteromorpha extract; (2) Mix the Bacillus subtilis powder, Bacillus amyloliquefaciens powder, and Bacillus megaterium powder in a mass-volume ratio of 1 g : 100mL was inoculated in the same LB liquid medium, and continuously cultivated on a shaker at 28°C and 90rpm for 24 hours to obtain the strain amplification liquid; (3) OD of the strain amplification liquid was obtained by using LB liquid medium 600 Adjust the strain value to 0.6 to obtain the strain standard solution; (4) Inoculate the strain standard solution into the extract of Enteromorpha enter...

Embodiment 2

[0024] Embodiment 2: a kind of preparation method of the bacillus preparation that promotes crop growth comprises the following steps: (1) enteromorpha powder is added into sterile deionized water according to mass volume ratio 1g: 100mL to obtain enteromorpha powder suspension, and above-mentioned Boil the Enteromorpha powder suspension for 40 minutes, adjust the pH value to 7.5 after cooling to room temperature, and obtain the Enteromorpha extract; (2) Bacillus subtilis powder, Bacillus amyloliquefaciens powder, and Bacillus megaterium powder were mixed in a mass-volume ratio of 1 g : 100mL was inoculated in the same LB liquid medium, and continuously cultivated on a shaker at 30°C and 120rpm for 24 hours to obtain the strain amplification liquid; (3) OD of the strain amplification liquid was obtained by using LB liquid medium 600 Adjust the strain value to 0.6 to obtain the strain standard solution; (4) inoculate the strain standard liquid into the extract of Enteromorpha en...

Embodiment 3

[0030]Embodiment 3: a kind of preparation method of the bacillus preparation that promotes crop growth comprises the following steps: (1) enteromorpha powder is added into sterile deionized water according to mass volume ratio 1g: 80mL to obtain enteromorpha powder suspension, and above-mentioned Boil the enteromorpha powder suspension for 35 minutes, adjust the pH value to 7.2 after cooling to room temperature, and prepare the enteromorpha extract; : 100mL was inoculated in the same LB liquid medium, and continuously cultivated on a shaker at 29°C and 105rpm for 24 hours to obtain the strain amplification liquid; (3) OD of the strain amplification liquid was obtained by using LB liquid medium 600 Adjust the value to 0.6 to obtain the strain standard solution; (4) Inoculate the strain standard solution into the extract of Enteromorpha enteromorpha at a volume ratio of 1:700, and continuously culture it on a shaker at 30°C and 100 rpm for 84 hours to obtain a fermentation liquid...

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Abstract

The invention belongs to the technical field of agricultural microbial preparations, in particular to a preparation method of a bacillus preparation for promoting crop growth. In the present invention, enteromorpha is heated and hydrolyzed to produce enteromorpha extract, which is used to amplify and obtain fermentation liquid containing Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus megaterium, and the fermentation liquid is freeze-dried and mixed with an appropriate amount of whey powder Afterwards, the bacillus preparation for promoting the growth of crops is prepared. The raw material of the invention is easy to obtain, the preparation process is stable, and the prepared bacillus preparation for promoting crop growth can effectively promote the growth of crops, and is suitable for popularization and application.

Description

technical field [0001] The invention belongs to the technical field of agricultural microbial preparations, in particular to a preparation method of a bacillus preparation for promoting crop growth. Background technique [0002] Plant growth-promoting bacteria are a group of microorganisms that mainly exist in plant roots. Different plant growth-promoting bacteria have different functions of promoting plant growth, these functions include biological nitrogen fixation, dissolution of phosphate in soil, improvement of plant stress resistance, promotion of plant absorption of trace elements, regulation of plant hormone secretion, etc. Studies have found that a variety of Bacillus bacteria belong to plant growth-promoting bacteria. For example, Bacillus megaterium has a good phosphorus-dissolving effect, and Bacillus cereus and Bacillus subtilis can effectively improve the disease resistance of plants. [0003] At present, various plant growth-promoting bacteria preparations ar...

Claims

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Application Information

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IPC IPC(8): A01N63/22A01P21/00C12N1/20C12R1/07C12R1/11C12R1/125
CPCA01N63/22C12N1/20
Inventor 邱彦国张艾青田相利
Owner CHANGYI MINGXING FEED CO LTD