Method for preventing and controlling aflatoxin and application of aspergillus flavus AFLA099790 gene in screening prevention and control drugs
A kind of technology of aflatoxin and aflatoxin, applied in the field of genetic engineering, can solve the problems of unknown effect and no one has reported the effect of NO, and achieve the effect of early prevention and control, pollution suppression and infection suppression
Pending Publication Date: 2021-09-17
XUZHOU NORMAL UNIVERSITY
View PDF0 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Although recent studies have found that filamentous fungi can also synthesize NO, which is involved in fungal growth and development, morphogenesis, asexual reproduction, and apoptosis, the role of NO in aflatoxin synthesis has not been reported yet.
There is no research report on the function of the AFLA_099790 gene in Aspergillus flavus, and its role in responding to NO and regulating aflatoxin is currently unknown
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment
[0022] 1. Fresh Spore Collection
[0023] Pipette 2 μL of wild-type Aspergillus flavus spores and inoculate them on PDA solid medium, and culture them in the dark at 37°C for 7 days; use 0.001% Tween 20 spore eluent to elute the spores, and filter the mycelia with four layers of lens-cleaning paper , Collect the conidia into a 10 mL centrifuge tube. Count the spores after dilution, and uniformly dilute the concentration of the spores to 10 7 individual / mL.
[0024] 2. The effect of nitric oxide NO on the growth and toxin synthesis of Aspergillus flavus
[0025] Add 1mM, 5mM and 10mM NO donors to the GMM medium respectively. After the medium is solidified, add 2 μL of the freshly collected Aspergillus flavus spores in the middle of the medium, and put them in a 29°C incubator for five days. From figure 1 As shown in A, NO can inhibit the growth of Aspergillus flavus, and 10mM NO can significantly inhibit the development of conidia and mycelial growth of Aspergillus flavus. ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention relates to a method for inhibiting synthesis of aflatoxin. An exogenous 10mM nitric oxide donor is added to reduce the expression level of an NO degrading enzyme encoding gene or an encoding protein thereof in aspergillus flavus so as to inhibit synthesis of aspergillus flavus aflatoxin. The application of the aspergillus flavus AFLA099790 gene in screening prevention and control drugs comprises the following steps: treating a host infected with aspergillus flavus by using a drug, and obtaining the drug capable of reducing the expression level of the aspergillus flavus based on AFLA099790 or AFLA099790 coding protein, namely the drug capable of preventing and controlling the aspergillus flavus to synthesize aflatoxin. A method for screening the drug for preventing and controlling aspergillus flavus to synthesize aflatoxin comprises the following steps: treating the host infected with aspergillus flavus by using the drug, and obtaining the drug capable of inhibiting the expression level of NO degrading enzyme genes in the aspergillus flavus or encoding protein of the NO degrading enzyme genes, namely the drug capable of preventing and controlling aspergillus flavus to synthesize aflatoxin. According to the method, pollution of aspergillus flavus to many agricultural products can be effectively inhibited, and the effects of prevention and control in advance and economic loss reduction are achieved.
Description
technical field [0001] The invention relates to genetic engineering, in particular to a method for preventing and controlling aflatoxin synthesis. Background technique [0002] Aspergillus flavus is a worldwide distribution of pathogenic bacteria, which can infect many important crops and animals. It not only causes huge economic losses to agricultural production, but also poses a huge threat to human health due to the food safety problems it causes. Aflatoxin B1 (AFB1), a secondary metabolite produced by it, is one of the most toxic and carcinogenic natural compounds found so far. The pollution of agricultural products by Aspergillus flavus and aflatoxin has become an economic and health problem, so it is of great significance to study the prevention and control of Aspergillus flavus pollution. [0003] Nitric oxide (Nitric Oxide, NO) is a fat-soluble, highly active free radical gas small molecule, which participates in a series of important signal transduction pathways an...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): A23B9/22
CPCA23B9/22A23V2002/00A23V2250/10
Inventor 杨坤龙孙统政孙文吴佳雯罗越赵炜耿青如王雨荷田俊
Owner XUZHOU NORMAL UNIVERSITY