Hanseniaspora pseudoquilliermondii and application thereof in yellow peach fruit wine
A technology for yeast and fruit wine, applied in the application, preparation of alcoholic beverages, methods based on microorganisms, etc., can solve the problems of not fully showing good quality, affecting the richness and diversity of local resources, and lack of typicality of products. Increase aroma complexity, increase fruit aroma, increase aroma effect
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Embodiment 1
[0036] A Hansenia sporapseudoquilliermondii of the present invention, the Hansenia sporapseudoquilliermondii is Hansenia sporapseudoquilliermondii HT9, its preservation number in the China Center for Type Culture Collection is CCTCC No: M 2021444, and the depository address is located at Preservation Center of Wuhan University, China, date of deposit is April 25, 2021.
[0037] Isolation, purification and identification of Hansenula spp. HT9:
[0038] Take 10g of the soil and the surface of the yellow peach fruit in 90mL YPD liquid medium, culture with shaking for 24 hours, take the supernatant to gradient dilution, spread on the YPD medium, and invert for 2-3 days. The yellow peach juice was squeezed and put into a sterile Erlenmeyer flask for fermentation, then spread on the YPD medium by gradient dilution, and cultivated for 2-3 days. The culture temperature was 28°C. Select colonies with typical yeast characteristics for streaking on plates, inoculate them on YPD slopes ...
Embodiment 2
[0043] A kind of application of the spore-bearing yeast of the present invention in the processing of yellow peach fruit wine, adopting the spore-bearing yeast of embodiment 1, comprises the following steps:
[0044] S1. Juicing of raw materials, color protection and enzymatic hydrolysis: After cleaning the yellow peaches, use high-concentration ozone combined with high-voltage pulse electric field to degrade pesticide residues. The cleaned yellow peaches will be subjected to high-voltage pulse electric field at the same time as the ozone cleaning treatment in the ozone pool. Treatment, the concentration of ozone in the ozone pool is 10mg / L, and the cleaning treatment time is 30min. The conditions for high-voltage pulse electric field treatment are: electric field strength 2kV / cm, pulse time 100μs, and pulse number 80. After treatment, the core is removed and beaten to obtain Yellow peach juice, taking the quality of yellow peach juice as a benchmark, by mass fraction, add 0.8%...
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