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Construction method and application of acute pancreatitis animal model

An acute pancreatitis, animal model technology, applied in animal husbandry and other directions, can solve problems such as drugs for animal models of pancreatitis that are difficult to study, difficult to clinically relevant, and achieve strong clinical relevance, simple modeling methods, high success rate

Active Publication Date: 2021-09-24
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the research on the specific association mechanism between the above factors and AP is still unclear, so it is difficult to effectively guide the establishment of animal models with high clinical relevance and the development of new drugs for the treatment of pancreatitis

Method used

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  • Construction method and application of acute pancreatitis animal model
  • Construction method and application of acute pancreatitis animal model
  • Construction method and application of acute pancreatitis animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, the construction of mouse model of acute pancreatitis

[0036]The mice were fed with high-fat diet continuously for 12 weeks, and the feed was added every week to ensure its quality. The high-fat feed used in this example is 60% high-fat feed (HFD, fat energy supply ratio is 60%, H10060, Huafukang, Beijing) in the obesity model series feed (DIO series Diets); the specific composition of the feed and See Table 1 for energy supply ratio. Alcohol induction was carried out after 12 weeks, and all mice were fasted and watered 12 hours before modeling. A 37.5% ethanol solution was prepared with physiological saline and used immediately after preparation, and the mice were given intraperitoneal injection of 37.5% ethanol solution (10 μL / g) for 2 consecutive injections with an interval of 1 hour. One hour after the last alcohol injection, the rats were rehydrated, that is, normal saline (10 μL / g) was injected intraperitoneally. Induction process see figure 1 . ...

experiment example 1

[0040] Experimental example 1. Obesity and alcohol synergistically induce pathological changes in mouse pancreas and damage to multiple organ functions

[0041] 1. Grouping and handling of animals (8 animals in each group)

[0042] (1) Obese alcoholic acute pancreatitis group (i.e. Example 1): fed with high-fat diet for 12 weeks, given 37.5% ethanol solution for 2 consecutive injections with an interval of 1 hour. Concrete operation is carried out according to embodiment 1.

[0043] (2) Blank control group: normal diet (use control feed (CD, fat energy supply ratio is 10%, H10010, Huafukang, Beijing), the specific composition and energy supply ratio of the feed are shown in Table 1) for 12 weeks, given and The ethanol solution used in Example 1 was injected with physiological saline equivalent to 2 consecutive times with an interval of 1 hour, and the rest of the operations were the same as in Example 1.

[0044] (3) High-fat control group: fed with a high-fat diet for 12 we...

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Abstract

The invention provides a construction method of an acute pancreatitis animal model. According to the construction method, high-fat feed and alcohol are used for jointly inducing animals to form acute pancreatitis. Obesity caused by high-fat feed and alcohol can synergistically cause pathological damage to pancreas of mice, local inflammatory response of pancreas and inflammatory response of lungs and the whole body. In a basic obesity state, the morbidity condition of a large amount of alcohol drinking of the animal model is closer to human morbidity, pancreatic injury is obvious and accompanied by multiple organ dysfunction syndromes, and the animal model accords with the clinical characteristic that obese acute pancreatitis patients are prone to severe illness. The animal model is simple in modeling method, good in repeatability and high in clinical correlation, the model can be used for studying the pathogenesis of obesity and alcohol-related acute pancreatitis, and an important basis is provided for screening treatment targets and studying medical transformation.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a construction method and application of an animal model of acute pancreatitis. Background technique [0002] Acute pancreatitis (AP) is an inflammatory injury such as pancreatic edema, hemorrhage and necrosis caused by the self-digestion of pancreatic tissue caused by various etiologies. With the change of people's lifestyle, the incidence of AP is increasing year by year. As a common disease in gastroenterology, AP is mainly manifested as acute and persistent mid-upper abdominal pain, accompanied by nausea, vomiting, etc., blood amylase or lipase increased more than 3 times the upper limit of normal value, accompanied by typical image In severe cases, the inflammation spreads to the whole body, often combined with systemic or local complications, and the prognosis is poor. [0003] It is extremely important to study the pathogenesis and drug treatment of AP, but there...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 杨鑫敏
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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