Aromatic microbial environment-friendly deodorant and preparation method thereof
An environmentally friendly deodorant and microbial technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of poor sustainability, easy adverse effects on the environment, poor treatment effect, etc., and achieve good synergy Effect
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Embodiment 1
[0036] An aromatic microbial environment-friendly deodorant, which is prepared by the following method:
[0037] Inoculate landfill leachate onto LB medium for microbial enrichment culture to obtain landfill leachate-enriched colonies, conduct activation culture and inoculate landfill leachate-enriched colonies into LB liquid medium, at 30°C, in Cultivate in a shaker at 170r / min for 30 hours to obtain the landfill leachate-enriched seed bacterial solution, the concentration of which is greater than 1×10 8 CFU / mL.
[0038] Inoculate yogurt on LB medium for microbial enrichment culture to obtain yogurt-enriched colonies, and perform activation culture on the yogurt-enriched colonies and inoculate them into LB liquid medium at 30°C in a 180r / min shaker Cultivate for 28 hours to prepare yogurt-enriched seed bacterial solution, the concentration of which should be 1×10 8 CFU / mL or more.
[0039] Inoculate the sweet wine on the PDA medium for microbial enrichment culture to obtai...
Embodiment 2
[0044] An aromatic microbial environment-friendly deodorant, which is prepared by the following method:
[0045] Inoculate landfill leachate onto LB medium for microbial enrichment culture to obtain landfill leachate-enriched colonies, conduct activation culture and inoculate landfill leachate-enriched colonies into LB liquid medium, at 30°C, in Cultivate in a shaker at 170r / min for 36 hours to obtain the landfill leachate-enriched seed bacterial solution, the concentration of which is greater than 1×10 8 CFU / mL.
[0046] Inoculate yogurt on LB medium for microbial enrichment culture to obtain yogurt-enriched colonies, and perform activation culture on the yogurt-enriched colonies and inoculate them into LB liquid medium at 30°C in a 180r / min shaker Cultivate for 30 hours to prepare the yogurt-enriched seed bacterial solution, and the bacterial concentration is required to be 1×10 8 CFU / mL or more.
[0047] Inoculate the sweet wine on the PDA medium for microbial enrichment...
Embodiment 3
[0052] An aromatic microbial environment-friendly deodorant, which is prepared by the following method:
[0053] Inoculate landfill leachate onto LB medium for microbial enrichment culture to obtain landfill leachate-enriched colonies, conduct activation culture and inoculate landfill leachate-enriched colonies into LB liquid medium, at 30°C, in Cultivate in a shaker at 170r / min for 26 hours to obtain the landfill leachate-enriched seed bacteria solution, and the concentration of the bacteria is greater than 1×10 8 CFU / mL.
[0054] Inoculate yogurt on LB medium for microbial enrichment culture to obtain yogurt-enriched colonies, and perform activation culture on the yogurt-enriched colonies and inoculate them into LB liquid medium at 30°C in a 180r / min shaker Cultivate for 32 hours to prepare yogurt-enriched seed bacterial solution, and the bacterial concentration is required to be 1×10 8 CFU / mL or more.
[0055] Inoculate the sweet wine on the PDA medium for microbial enri...
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