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Probiotic strain for aquatic feeding and preparation method for microbial agent of probiotic strain

A technology of bacterial agents and aquatic animals, applied in the field of microorganisms, can solve problems such as inability to exert the best beneficial effect

Active Publication Date: 2021-09-24
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, many strains with good probiotic effects in livestock and poultry are directly applied to aquaculture, but probiotics are host-specific, and probiotics derived from livestock and poultry cannot colonize well because they are not suitable for the intestinal environment of aquatic animals Probiotics from the same species or their natural environment may be the best way to improve the probiotic function. There have been many studies on the application of probiotics from aquatic sources to aquaculture and achieved good effects. the case

Method used

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  • Probiotic strain for aquatic feeding and preparation method for microbial agent of probiotic strain
  • Probiotic strain for aquatic feeding and preparation method for microbial agent of probiotic strain
  • Probiotic strain for aquatic feeding and preparation method for microbial agent of probiotic strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1, the isolation and identification of Bacillus velezensis T23

[0058] In this study, Bacillus velezensis T23 was isolated and used as an indigenous strain of fish intestinal tract. Specifically, under aseptic conditions, slowly extrude the intestinal contents from the hindgut to the anterior intestine of the fish intestine, place them in a 50mL centrifuge tube containing 10mL of PBS buffer, mix well and let it stand for 1 minute, then take the upper Clearance was serially diluted, taking 10 -5 ~10 -9TSA medium (15g / L trypsin digest of casein, 5g / L papain digest of soybean powder, 5g / LNaCl, 15g / L agar) was coated with 100μL of the dilution gradient of TSA medium, and after 24 hours of cultivation, the colonies were picked and streaked Purification, the purified single colony was inoculated into the liquid medium, and after culturing for 15-16 hours, an appropriate amount of the bacterial liquid was sent to a sequencing company for 16S rDNA sequencing, and the...

Embodiment 2

[0060] Embodiment 2, Bacillus Velez T23 promotes the growth of zebrafish and reduces the feed factor

[0061] One, the preparation of Bacillus Velez T23 inoculum

[0062] 1. Strain activation: Bacillus Velez T23 was stored in an ultra-low temperature refrigerator, streaked on an LB solid plate, and cultured overnight at 30°C;

[0063] 2. Primary seed culture: Pick a single colony in LB (Luria-Bertani) medium, culture at 30°C, 180r / min for 24h;

[0064] 3. Secondary seed liquid culture: Inoculate in LB medium according to 1% inoculum amount, and cultivate at 30°C and 180r / min for 12h;

[0065] 4. Solid fermentation: inoculate the secondary seed solution in a solid medium with 5% inoculum amount; culture in a shaker at 30° C. for 48 hours to obtain the culture of T23, that is, the T23 fermentation product, which is the Bacillus velei T23 inoculum. In the Bacillus Velez T23 inoculum, the content of Bacillus Velez T23 is 8×10 8 cfu / g.

[0066] Preparation method of solid ferme...

Embodiment 3

[0073] Example 3, Bacillus Velez T23 improves the intestinal morphology of zebrafish

[0074] After the breeding experiment, the zebrafish treated by the Bacillus group and the zebrafish treated by the control group in Example 2 starved for 24 hours were dissected on ice, and the complete intestinal tract was taken out and put into a centrifuge tube containing 4% paraformaldehyde Fixation and HE staining were done by Beijing Zhongke Wanbang Biotechnology Co., Ltd., and photographing was done in our laboratory (the software is ZEN). The length of intestinal villi and the thickness of muscular layer were measured by ImageJ software.

[0075] The results showed that the addition of Bacillus Velez T23 in the Bacillus group significantly increased the length of the intestinal villi and the thickness of the muscular layer in the zebrafish compared with the control group ( figure 2 ) (P<0.01), indicating that Bacillus Velez T23 can improve the intestinal health of fish by improving...

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Abstract

The invention discloses a probiotic strain for aquatic feeding and a preparation method for a microbial agent of the probiotic strain. The probiotic strain for aquatic feeding is a novel bacillus velezensis strain, has a strain number of T23 and has an accession number of CGMCC No. 22029 in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms. Experiments prove that the strain T23 can be used for promoting the growth of aquatic animals, reducing a feed coefficient, maintaining the intestinal health of the aquatic animals and improving the disease resistance of the aquatic animals, and has a broad application prospect in aquaculture.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a probiotic strain for aquatic feeding and a preparation method of a bacterial agent thereof. Background technique [0002] Probiotics are live microorganisms that, in appropriate doses, have a beneficial effect on the health of the host. Bacillus is the most widely used and best-studied probiotic strain, but most of it is concentrated on Bacillus subtilis and Bacillus licheniformis, lacking other species with good efficacy. [0003] At present, many strains with good probiotic effects in livestock and poultry are directly applied to aquaculture, but probiotics are host-specific, and probiotics derived from livestock and poultry cannot colonize well because they are not suitable for the intestinal environment of aquatic animals Probiotics from the same species or their natural environment may be the best way to improve the probiotic function. There have been many studies on the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K50/80A23K10/18A23K10/16C12R1/07
CPCC12N1/20A23K50/80A23K10/18A23K10/16
Inventor 周志刚杨雅麟胡娟张震药园园冉超杜东东高辰辰夏锐张凤丽
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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