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Stainless steel ultrasonic sheet chemiluminescence solution detection device and using method thereof

A technology of chemiluminescence and detection devices, which is applied in the directions of chemiluminescence/bioluminescence, measuring devices, and analysis by making materials undergo chemical reactions, which can solve the problems of restricting wide application, expensive devices, and large volume, and achieve convenient use, Create cheap, small effects

Active Publication Date: 2021-09-24
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, both devices are expensive, bulky, and energy-intensive, which greatly limits their widespread application in sonochemiluminescence.

Method used

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  • Stainless steel ultrasonic sheet chemiluminescence solution detection device and using method thereof
  • Stainless steel ultrasonic sheet chemiluminescence solution detection device and using method thereof
  • Stainless steel ultrasonic sheet chemiluminescence solution detection device and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Preparation of stainless steel ultrasonic sheet chemiluminescence solution detection device

[0077] On the basis of the microporous stainless steel atomizing sheet of the household USB humidifier, the porous stainless steel sheet on the stainless steel microporous atomizing sheet is replaced with a non-porous stainless steel sheet, and a ring-shaped plastic insulating sheet is attached to the non-piezoelectric ceramic ring surface to form Grooves with a diameter of 10mm.

[0078] Then add the chemiluminescent solution (luminol solution with a certain gradient in the range of 0.5-200 μM, and the above-mentioned luminol solution and 25 μM hydrogen peroxide solution) into the groove respectively, the addition amount is generally 30-50 μL and then pass Ultrasonic vibration of the stainless steel ultrasonic plate causes ultrasonic chemiluminescence.

[0079] The ultrasonic chemiluminescence process of the chemiluminescence solution detected by the chemiluminescence solutio...

Embodiment 2

[0083] The relationship between the pH of CBS buffer and the intensity of ultrasonic chemiluminescence (SCLIntensity).

[0084] Example of pH Condition Optimization

[0085] Configure different pH detection solutions (the pH gradient is: 9.4, 10, 10.5, 11, 11.5, 12, 12.5, 13), add the mixed solution in the reaction groove on the reverse side of the present invention, and use ultrasonic action to cause ultrasonic chemiluminescence.

[0086] see Figure 4 , Figure 4 It is the relationship curve between the pH of the CBS buffer solution and the intensity of ultrasonic chemiluminescence (SCLIntensity) in Example 2 of the present invention.

[0087] When the pH=12 of CBS buffer [other experimental conditions: c(Luminol)=100μM, c(H 2 o 2 )=25 μ M], the luminous intensity reaches the maximum value, and the pH intensity continues to increase and decreases instead, so the pH value of the CBS buffer solution can be selected as 12 when detecting hydrogen peroxide and glucose concent...

Embodiment 3

[0089] Relationship between hydrogen peroxide concentration and SCL intensity

[0090] When the pH of the CBS buffer was 12 [other experimental conditions: c (Luminol) = 100 μ M], the luminous intensity reached the maximum value, and continued to increase the pH intensity but decreased. Choose pH 12.

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Abstract

The invention provides a chemiluminiscence solution detection device. The chemiluminiscence solution detection device comprises a nonporous stainless steel atomization sheet and a chemiluminiscence solution storage tank arranged on a back surface of the nonporous stainless steel atomization sheet. On the basis of a piezoelectric ultrasonic transducer with a microporous stainless steel atomization sheet, a stainless steel sheet without holes is used for replacing a porous microporous stainless steel sheet, and ultrasonic chemiluminescence is generated after a chemiluminescence solution is added. According to the device, a chemiluminescence solution can be prevented from leaking through the micropores, and the defect that atomization of the chemiluminescence solution is not beneficial to chemiluminescence detection is effectively inhibited so that long-time ultrasonic chemiluminescence detection can be realized. the ultrasonic device can obviously enhance luminescence, so the solution which does not generate chemiluminescence or generates weak chemiluminescence under the condition of no ultrasound can generate stronger light, and thus the solution can be observed through human eyes, smart phones or related instruments. The chemiluminescence solution detection device is low in manufacturing cost, low in driving voltage, small in size and convenient to use.

Description

technical field [0001] The invention belongs to the technical field of chemiluminescence solution detection, and relates to a chemiluminescence solution detection device and a use method thereof, in particular to a stainless steel ultrasonic sheet chemiluminescence solution detection device and a use method thereof. Background technique [0002] Sonic chemiluminescence (SCL) refers to the light emission produced by the chemical reaction of sonolysis products produced by cavitation bubbles. Compared with chemiluminescence (CL) and electrochemiluminescence (ECL), sonochemiluminescence has some unique advantages. In a typical sonochemiluminescence system, reactive oxygen species can be generated in situ by ultrasound without adding other auxiliary reagents. Therefore, sonochemiluminescence has received more and more attention. [0003] So far, several types of ultrasound equipment have been used for SCL research. One is a horn-type piezoelectric ultrasonic transducer with a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N21/01
CPCG01N21/76G01N21/01G01N2021/0112
Inventor 孟成达徐国宝
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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