Microecological green preservative for waxberries and preparation method of microecological green preservative
A preservative and micro-ecological technology, applied in the preservation of food ingredients as anti-microbials, food ingredients as coating agents, protection of fruits/vegetables with a protective layer, etc., can solve problems such as short storage period and prolong freshness Period, low cost, easy to promote the effect
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Embodiment 1
[0074] The preparation method of bayberry micro-ecological preservative is as follows: prepare the fermentation medium according to the following composition, and the medium formula is: 5.16% of mulberry branch powder, 2.38% of wheat bran, 4.85% of rice bran, 0.094% of potassium dihydrogen phosphate, and 0.054% of magnesium sulfate. %, the rest was tap water, and the culture medium was sterilized at 121°C for 20 minutes in a sterilizing pot. Inoculate Phellinus seed liquid with 5% of the fermentation liquid volume, shake the flask culture at 28°C and 120r / min, stop the fermentation when the number of bacterial balls reaches 2 / mL, and crush it with a tissue grinder under aseptic conditions until the bacteria Until the ball disappears, culture at 28°C for 6 hours, inoculate wild yeast seed liquid with 5% of the volume of the fermentation liquid, and culture in a shaker flask at 25°C and 130r / min for 16 hours, add 60mL of okra polysaccharide extract and 12g Chitosan is stirred an...
Embodiment 2
[0077] The preparation method of bayberry micro-ecological preservative is as follows: prepare the fermentation medium according to the formula, and the medium formula is: 5.16% of mulberry powder, 2.38% of wheat bran, 4.85% of rice bran, 0.094% of potassium dihydrogen phosphate, and 0.054% of magnesium sulfate , the rest is tap water, and the culture medium is sterilized at 121°C for 20 minutes in a sterilizing pot. Inoculate Phellinus seed liquid with 5% of the fermentation liquid volume, shake the flask culture at 28°C and 120r / min, stop the fermentation when the number of bacterial balls reaches 3 / mL, and crush it with a tissue grinder under aseptic conditions until the bacteria Until the ball disappears, culture at 28°C for 8 hours, inoculate wild yeast seed liquid with 5% of the fermentation liquid volume, shake the flask for 18 hours at 25°C and 130r / min, add 80mL okra polysaccharide extract and 10g Chitosan is stirred and dissolved and filtered through 4 layers of gauz...
Embodiment 3
[0080]The preparation method of bayberry micro-ecological preservative is as follows: prepare the fermentation medium according to the formula, and the medium formula is: 5.16% of mulberry powder, 2.38% of wheat bran, 4.85% of rice bran, 0.094% of potassium dihydrogen phosphate, and 0.054% of magnesium sulfate , the rest is tap water, and the culture medium is sterilized at 121°C for 20 minutes in a sterilizing pot. Inoculate Phellinus seed liquid with 5% of the fermentation liquid volume, shake the flask culture at 28°C and 120r / min, stop the fermentation when the number of bacterial balls reaches 1 / mL, and crush it with a tissue grinder under aseptic conditions until the bacteria Until the ball disappears, culture at 28°C for 10 hours, inoculate wild yeast seed liquid with 5% of the volume of the fermentation liquid, and culture in a shaker flask at 25°C and 130r / min for 15 hours, add 90mL of okra polysaccharide extract and 12g Chitosan is stirred and dissolved and filtered ...
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