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Collagen-based hydrogel for cartilage tissue engineering and preparation method thereof

A cartilage tissue and hydrogel technology, which is applied in the field of collagen-based hydrogel for cartilage tissue engineering and its preparation, can solve the problems of unsatisfactory long-term effect of cartilage repair, few sources of chondrocyte donors, irregular cartilage defects, and the like, To achieve the effect that is conducive to mass transfer

Pending Publication Date: 2021-10-08
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Therefore, the present invention provides a collagen-based hydrogel with good physical and chemical properties and biocompatibility, which can transform sol-gel in situ, and can induce stem cells to differentiate into cartilage, so as to effectively solve the problem of cartilage defects in clinical practice. Regularity, unsatisfactory long-term effect of cartilage repair, few sources of chondrocyte donors, and easy dedifferentiation

Method used

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  • Collagen-based hydrogel for cartilage tissue engineering and preparation method thereof
  • Collagen-based hydrogel for cartilage tissue engineering and preparation method thereof
  • Collagen-based hydrogel for cartilage tissue engineering and preparation method thereof

Examples

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Embodiment 1

[0060] A collagen-based hydrogel for cartilage tissue engineering, the hydrogel includes collagen and polysaccharides, the mass ratio of the collagen to the polysaccharide is 11:1 to 1:2, and the combination of the collagen and the polysaccharide is Physical cross-linking and chemical cross-linking, the concentration of the collagen-polysaccharide hydrogel is 3 mg / mL-20 mg / mL.

[0061] Further, the collagen includes one or more combinations of animal-derived type I collagen, animal-derived type II collagen or humanized collagen.

[0062] Further, the collagen is methacrylic anhydride-modified collagen, and the degree of substitution of the modified collagen is 20%-80%, preferably 30-50%.

[0063] Further, the polysaccharide is a modified polysaccharide, and the modified polysaccharide includes hyaluronic acid and / or chondroitin sulfate. Wherein, the molecular weight of hyaluronic acid in the modified polysaccharide is 20KDa-2000KDa, and the degree of substitution of the modif...

Embodiment 2

[0071] In one embodiment, the preparation of the hydrogel comprises the following steps:

[0072] S1, prepare modified collagen solution;

[0073] S2, preparing a modified polysaccharide solution;

[0074] S3. Mix the pure collagen hydrogel prepared in step S1 with the modified polysaccharide solution prepared in step S2 in proportion, and place it to stand at 34-40° C., so as to prepare the collagen-polysaccharide hydrogel.

[0075] Preferably, the concentration of the modified collagen solution is 10-25 mg / mL.

[0076] Preferably, the concentration of the modified polysaccharide solution is 10-40 mg / mL.

[0077] Preferably, the concentration of the collagen-polysaccharide hydrogel is 3 mg / mL-20 mg / mL.

[0078] Preferably, the gelation speed of the hydrogel is controlled by adjusting the ratio of the collagen to the polysaccharide or by adjusting the grafting rate of the polysaccharide.

[0079] Preferably, the hydrogel has a certain swelling property, and the swelling ra...

Embodiment 3

[0102] This embodiment is carried out on the basis of the above-mentioned embodiments 1 and 2, and the similarities with the above-mentioned embodiments 1 and 2 will not be repeated.

[0103] This example mainly introduces the preparation method of the controllable collagen-based hydrogel, and the steps are as follows:

[0104] S301. Dissolving the modified hyaluronic acid in Example 2 in PBS solution to form a HA-CHO solution with a concentration of 20%wt;

[0105] S302. Adjust the 20 mg / mL type 1 collagen solution to neutral with NaOH solution in an ice-water bath, mix the collagen solution and the aldylated hyaluronic acid solution at a mass ratio of 4:1, and then place it in a mold and incubate at 37°C Gel.

[0106] The preparation method of this embodiment has simple operation steps, and the preparation conditions are relatively easy to control.

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Abstract

The invention relates to collagen-based hydrogel for cartilage tissue engineering. The collagen-based hydrogel is characterized in that the hydrogel comprises collagen and polysaccharide; the collagen comprises one or a combination of more of animal source type I collagen, animal source type II collagen or humanized collagen; the polysaccharide comprises one or two of chondroitin sulfate and hyaluronic acid; and the combination mode of the collagen and the polysaccharide is physical cross-linking and / or chemical cross-linking. The hydrogel in cartilage tissue engineering has the effects of repairing irregular defects through direct injection, cooperatively repairing cartilage layer defects as freeze-dried sponge and cooperatively repairing cartilage layer defects as a cell delivery carrier wrapping cartilage cells and / or stem cells.

Description

technical field [0001] The invention relates to the field of biomaterials, in particular to a collagen-based hydrogel for cartilage tissue engineering and a preparation method thereof. Background technique [0002] Cartilage damage is mainly due to trauma, age-related degeneration, and conditions such as osteoarthritis. However, there is no vascular tissue, lymphatic drainage and innervation inside the cartilage tissue, and the renewal rate of the matrix is ​​slow. Once damaged, it is difficult to repair by itself. Current treatment methods mainly include microfracture in situ induced cartilage repair and matrix-associated autologous chondrocyte transplantation. Although some satisfactory results can be obtained by using these methods, long-term regeneration of cartilage tissue is still not satisfied. [0003] Hydrogels have attracted much attention for their potential applications as cell delivery vehicles. The application of hydrogels as volume fillers and cell carriers...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/50A61L27/52A61L27/56C08J3/075C08J3/24C08L89/00C08L5/08
CPCA61L27/26A61L27/50A61L27/52A61L27/56C08J3/075C08J3/246C08J3/243A61L2430/06A61L2300/412A61L2400/06C08J2389/00C08J2405/08C08J2305/08C08J2489/00C08L89/00C08L5/08
Inventor 郭立坤高勇丽樊渝江张兴栋
Owner SICHUAN UNIV
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