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An entomopathogenic nematode symbiotic bacterium with broad-spectrum antibacterial function and its application

An entomopathogenic nematode, broad-spectrum antibacterial technology, applied in the field of microorganisms, can solve problems such as no reports of biological pesticides

Active Publication Date: 2022-03-18
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the antibacterial compounds of this bacterium are mainly used in medicine, and there are no reports of their development as biological pesticides.

Method used

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  • An entomopathogenic nematode symbiotic bacterium with broad-spectrum antibacterial function and its application
  • An entomopathogenic nematode symbiotic bacterium with broad-spectrum antibacterial function and its application
  • An entomopathogenic nematode symbiotic bacterium with broad-spectrum antibacterial function and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: strain identification

[0018] XBD102 is a pathogenic symbiotic bacterium of Pseudobicornus elegans isolated from soil samples collected in Liaoning Province, China in our laboratory. The 16S rRNAgene of this strain was detected for bacterial species identification. The PCR amplification primers used were:

[0019] Upstream primer: 27F: 5'-AGAGTTTGATCCTGGCTCAG-3';

[0020] Downstream primer: 1492R:5'-GGTTACCTTGTTACGACTT-3';

[0021] The vector was constructed and transformed into Escherichia coli, and the 16s rDNA sequence alignment was performed, the results were as follows figure 1 shown, from figure 1 It can be seen that a strain of Xenorhabdus budapestensis was isolated and screened in this example, named XBD102.

Embodiment 2

[0022] Embodiment 2: the mensuration of antibacterial activity of XBD102 fermented liquid

[0023] Use NBTA agar medium (tryptone 10g / L; beef powder 3g / L; sodium chloride 5g / L; triphenyl tetrazolium chloride 0.04g / L; bromothymol blue 0.025g / L, pH=7.8 ) to XBD102, cultured at 28°C for 48 hours, blue-brown smooth colonies can be seen. Pick a single colony to LB medium (tryptone 10g / L; yeast extract 5g / L; sodium chloride 10g / L), 28°C; 200rpm for 14 hours to make a fermented seed liquid, with 2% inoculum Connect the seed liquid to the Erlenmeyer flask containing LB liquid medium, ferment for 48 hours under the above-mentioned conditions, take the supernatant by high-speed centrifugation, and filter and sterilize it with a sterile filter (0.22 μm), the aseptic fermentation supernatant is Bacteriostasis test liquid to be tested.

[0024] 1. Identification of fungal inhibitory activity

[0025] Get 1ml of sterile fermentation supernatant and 9ml of melted PDA agar medium (potato 2...

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Abstract

The invention relates to an entomopathogenic nematode symbiotic bacterium XBD102 (Xenorhabdus budapestensis) strain with broad-spectrum antibacterial function. The XBD102 strain is preserved in the General Microbiology Center (CGMCC) of the China Microbiological Culture Collection Management Committee (CGMCC), and the preservation number is CGMCC No: 22057 . The fermentation supernatant of the XBD102 strain has growth inhibitory effect on various fungi and bacteria, and can be used for the development and application of new biological pesticides.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an entomopathogenic nematode symbiotic bacterium with broad-spectrum antibacterial function and application thereof. Background technique [0002] Entomopathogenic nematode symbionts are Gram-negative bacteria that exist in the intestinal tract of entomopathogenic nematodes, belonging to the γ-subclass of Proteobacteria, including two genera Xenorhabdus and Photorhabdus, They are mutualistic and symbiotic with pathogenic nematodes in the infection stage of Steinerne sterni and Heterobacter nematode (Forst et al., 1997): After the pathogenic nematodes infect the insects, they release the symbiotic bacteria into the insect body cavity, and the symbiotic bacteria reproduce in large numbers and produce toxins and hydrolytic extracellular enzymes, eventually leading to the death of the host insect. [0003] Studies have shown that entomopathogenic nematode symbiotic bacteria c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P1/00A01P3/00C12R1/01
CPCC12N1/20A01N63/20
Inventor 李广悦李琲琲杨秀芬任杰曾洪梅袁京京
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI