Decellularized vascular matrix and preparation method thereof

A decellularized and vascular technology, which is applied in the field of decellularized vascular matrix and its preparation, can solve the problems of destroying the ultrastructure of ECM, and the mechanical and biological properties of the acellular matrix are difficult to meet the clinical treatment effect, so as to achieve good decellularized effect Effect

Pending Publication Date: 2021-10-22
浙江华臻医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using the above-mentioned chemical methods or physical methods or a combination of both still alters the ECM, destroys the ECM ultrastructure, leads to the degradation of extracellular matrix components such as collagen, glycosaminoglycans, growth factors, etc., and the mechanical properties of the acellular matrix And biological performance is difficult to meet the clinical treatment effect

Method used

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  • Decellularized vascular matrix and preparation method thereof
  • Decellularized vascular matrix and preparation method thereof
  • Decellularized vascular matrix and preparation method thereof

Examples

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Effect test

Embodiment 1

[0043] Example 1 Preparation of heterogeneous decellularized vascular matrix

[0044] This embodiment provides a method for preparing a heterogeneous decellularized vascular matrix, comprising the following steps:

[0045] (1) Pre-treat the vascular tissue to be used, add the vascular tissue into ultrapure water under the ultrasonic conditions of frequency 35KHZ, temperature 41°C, wash twice, 5 minutes each time;

[0046] (2) Dissolve Raptinal in DMSO to make a mother solution containing 20mmol / L Raptinal, and then use MinimumEagle's Medium (MEM) medium to dilute the mother solution containing 20mmol / L Raptinal into a working solution containing 10μmol / L Raptinal , incubate the vascular tissue to be used in the working solution containing 10 μmol / L Raptinal for 24 hours at room temperature (25°C);

[0047] (3) Then add the vascular tissue to be used in step (2) into ultrapure water under the ultrasonic conditions of frequency 35KHZ, temperature 41°C, and wash twice for 5 minu...

Embodiment 2

[0055] Example 2 Preparation of heterogeneous decellularized vascular matrix

[0056] This embodiment provides a method for preparing a heterogeneous decellularized vascular matrix, comprising the following steps:

[0057] (1) Pre-treat the vascular tissue to be used, add the vascular tissue into ultrapure water, the ultrasonic condition is frequency 25KHZ, temperature 45°C, wash 2 times, 5 minutes each time;

[0058] (2) Dissolve Raptinal in DMSO to make a mother solution containing 20mmol / L Raptinal, and then use MinimumEagle's Medium (MEM) medium to dilute the mother solution containing 20mmol / L Raptinal into a working solution containing 10μmol / L Raptinal , incubate the vascular tissue to be used in the working solution containing 10 μmol / L Raptinal for 26 hours at room temperature (25°C);

[0059] (3) Then add the vascular tissue to be used in step (2) into ultrapure water under the ultrasonic condition of frequency 25KHZ, temperature 45°C, wash once, 5 minutes each time...

Embodiment 3

[0067] Example 3 Preparation of heterogeneous decellularized vascular matrix

[0068] This embodiment provides a method for preparing a heterogeneous decellularized vascular matrix, comprising the following steps:

[0069] (1) Pretreat the vascular tissue to be used, and add the vascular tissue into ultrapure water under the ultrasonic condition of frequency 45KHZ, temperature 37°C, wash 3 times, 5 minutes each time;

[0070] (2) Dissolve Raptinal in DMSO to make a mother solution containing 20mmol / L Raptinal, and then use MinimumEagle's Medium (MEM) medium to dilute the mother solution containing 20mmol / L Raptinal into a working solution containing 10μmol / L Raptinal , incubate the vascular tissue to be used in the working solution containing 10 μmol / L Raptinal for 24 hours at room temperature (25°C);

[0071] (3) Then add the vascular tissue to be used in step (2) into ultrapure water under the ultrasonic condition of frequency 45KHZ, temperature 37°C, wash 3 times, 7 minute...

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Abstract

The invention relates to the field of tissue engineering, in particular to a decellularized vascular matrix and a preparation method thereof. In the decellularization treatment step, a working solution containing [9, 9 '-di-9H-fluorene]-9, 9'-diformaldehyde is used for incubating vascular tissue organs to be used so as to remove cells, and the decellularized vascular matrix has huge application potential in reduction of decellularization time, moreover, when all cells in the outer membrane, the middle membrane and the inner membrane of the blood vessel are completely removed, a complete three-dimensional structure and extracellular matrix components are reserved, and low immunogenicity, low cytotoxicity and good mechanical properties are achieved.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a decellularized vascular matrix and a preparation method thereof. Background technique [0002] Surveys show that cardiovascular disease is the leading cause of death worldwide. At present, the main treatment methods for cardiovascular diseases mainly include the following four types, namely drug therapy, lifestyle adjustment, interventional therapy and coronary artery bypass grafting. Among them, the graft vessels used in coronary artery bypass grafting mainly include autologous blood vessels and artificial blood vessels. Autologous blood vessels are the gold standard for vascular bypass graft materials. The commonly used autologous blood vessels mainly include the internal mammary artery, radial artery and great saphenous vein, but the sources of autologous blood vessels are limited and cause secondary trauma to patients. In addition, many patients suffer from Other vascula...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3625A61L27/3687A61L27/3691A61L27/507
Inventor 赵子建王建英
Owner 浙江华臻医疗器械有限公司
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